Health, Safety & Environment Annual Containment Level 2 Laboratory Safety Checklist (Appendix 8 of Biosafety Program) To be completed by the Biosafety Committee Laboratory Manager Name: Building and Room Number: Inspected by: Date: The following inspection report identifies deficiencies found by the Biosafety Committee. This inspection report is in addition to the annual Local Safety Committee Checklist completed for every University of Regina laboratory. A. ADMINISTRATION OF BIOSAFETY 1. Detailed Biosafety Questionnaire completed (containment level/risk assessment) 2. Health and medical surveillance program in place 3. Risk assessment completed to determine which procedures what concentrations and volumes necessitate the use of a BSC 4. Documentation of site- and project-specific training kept on file B. BIOSECURITY 5. Laboratory follows the appropriate level of biosecurity as outlined in the UR Biosecurity Plan, including training 6. Biological inventory current (<1 year) C. MANAGEMENT OF BIOSAFETY 7. Emergency procedures posted and legible (fire, spills, injuries, SOPs, BSC failure) 8. PSDS information posted 9. Biosafety Program Manual available YES NO NA COMMENTS YES NO NA COMMENTS YES NO NA COMMENTS 10. CL2 Standard Operating Procedures available 11. Biological Safety Cabinet Procedures available 12. Hands-free washing sinks located near the laboratory exit (soap and paper towel accessible) 13. Shower available and accessible 14. Eyewash available and accessible 15. CL2 spill kit available and stocked 2012-03 page 1 D. AUTHORIZED ENTRY YES NO NA COMMENTS YES NO NA COMMENTS YES NO NA COMMENTS YES NO NA COMMENTS 16. Personnel (visitors, trainees, and others) are required to wear protective laboratory clothing when entering/working in the laboratory 17. Access to laboratory limited to authorized personnel 18. Appropriate CL2 Biohazard Sign on laboratory door 19. Laboratory doors kept closed and locked 20. Record made of other people (emergency responders) entering facility during an emergency 21. Visitors, maintenance staff, custodial staff and others provided with training and/or supervision with regards to anticipated activites in laboratory E. PERSONAL PROTECTION 22. Procedures in place to ensure that substantial footwear is worn and that legs are covered 23. Procedures in place outling protective laboratory clothing requirements and decontamination procedures 24. Laboratory coats and gloves available 25. Face shield and eye protection available and in good condition 26. Respirator(s) available 27. Respirator user(s) trained & fit-tested F. HOUSEKEEPING 28. Bench tops and sink areas tidy 29. Food and drink absent 30. “No Eating/Drinking/Smoking” signs posted G. DECONTAMINATION 31. Procedures in place for decontamination of bench tops and surfaces at end of working day 32. Procedures in place for decontamination of laboratory rooms and large pieces of equipment 33. Procedures in place for contaminated materials and equipment to be decontaminated before disposal or removal from laboratory 34. Disinfectants are effective against agents in use and stored in all areas where agents are used 2012-03 Annual Containment Level 2 Lab Safety Checklist page 2 H. WASTE YES NO NA COMMENTS YES NO NA COMMENTS 35. Biological waste containers labelled and secure 36. “Biohazardous waste” segregated from general refuse 37. Needles and sharps in “Sharps” container 38. Procedures in place for effective decontamination (autoclave, disinfectant) I. BIOLOGICAL SAFETY CABINETS 39. BSC certified in accordance with NSF/ANSI 49-2004s: NSF 49 Class II (Laminar Flow) Biosafety Cabinetry requirements 40. Laboratory Managers have current BSC certification report on file Please ensure that a written response and corrections are made by: (Date) Principal Investigator Signature: Date: Biosafety Officer Signature: Date: (Please sign after violations have been acted upon) UPON CORRECTION OF VIOLATIONS, Please submit completed form: by mail to Biosafety Officer, Health, Safety & Environment, Human Resources or, by e-mail to health.safety@uregina.ca (Attention: Biosafety Officer) 2012-03 Annual Containment Level 2 Lab Safety Checklist page 3