Joyce Pieretti Permanent Address: 5114 S. Greenwood Ave. Apt 3 Chicago, IL 60615 Phone: 908-283-4590 E-mail: jpieretti@uchicago.edu Education Ph.D. University of Chicago, Department of Organismal Biology & Anatomy (2015) B.A. Barnard College, Columbia University, Biology May 2009 Research Experience • University of Chicago o Graduate Student, Advisor: Dr. Neil Shubin Researching the functional diversification of a Sonic Hedgehog regulatory element in fins. Performing Danio rerio transgenesis using microinjection and fluorescent microscopy to assess reporter expression. Performing in situ hybridizations and bead implantations in a non-model organism, the skate, Leucoraja erinacea. Performing D. rerio care & mating. • Hunter College, CUNY o June 2009 – July 2010 Laboratory Technician Researched regulatory elements controlling classswitch recombination in transgenic Mus musculus. Analyzed antibody switching using B cells collected from spleens, cell culturing, and flow cytometry. Maintained laboratory conditions in compliance with university and NY State mandates. Mentored rotating graduate students. • Barnard College, Columbia University o September 2010 – Present Hughes Science Pipeline Research Intern Researched the molecular evolution of Clavata 2 in Arabidopsis thaliana and related species. Cloned clv2 orthologs using DNA extraction, PCR, transformation, and gel electrophoresis. Assisted in laboratory maintenance by preparing media plates, stock solutions, and plant propagation & care. June 2008 – June 2009 Joyce Pieretti Page 2 Teaching Experience • Upward Bound Tutor o Assisted high school students from Chicago Public Schools in biology, mathematics, and Spanish homework assignments. o Edited students’ writing for college applications. • Supplemental Instruction Leader o Developed and provided useful study strategies for students. o Promoted understanding of lecture material by generating discussion questions, posters, and assignments. o Provided a relaxed, but productive atmosphere for students to study with classmates and comfortably ask questions. • Classroom Assistant o As part of a Science in the City course, assisted a thirdgrade teacher in P.S. 76 in teaching the science curriculum specified by NY State, three hours a week. o Developed engaging bilingual science activities for students. • Introductory Biology Lab Assistant o Graded weekly lab quizzes. o Assisted students in understanding proper lab safety procedures. o Presented the lab protocol for students and demonstrated helpful techniques. Publications/Presentations • Li, F. Yan, Y., Pieretti, J., Feldman, D.A., Eckhardt, L.E. (2010). Comparison of Identical and Functional Igh Alleles Reveals a Nonessential Role for Eµ in Somatic Hypermutation and ClassSwitch Recombination. Journal of Immunology. doi:10.4049/jimmunol.0902992. • Yan, Y., Pieretti J., Christin J., Bah, F., Eckhardt, L.E. (2011). Homologous elements hs3a and hs3b in the 3′ regulatory region of the murine immunoglobulin heavy chain (Igh) locus are both dispensable for class-switch recombination. The Journal of Biological Chemistry 286: 27123-27131. September 2010 – December 2010 September 2008 – May 2009 September 2007 – December 2007 September 2006 – December 2006 Joyce Pieretti Page 3 • Hughes Science Pipeline Project. 17th Annual Student Research Symposium, April 21, 2009. Barnard College, NY, NY. Skills/Personal Aspects • Knowledge of Word, PowerPoint, and Excel. • Fluent in Spanish. • Work well independently and as a member of a group. • Comfortable with teaching biology to others. Miscellaneous Experience • City Park Counselor for Perth Amboy Department of Recreation July 2006 – August 2006 o Supervised young children while participating in activities such as playing board games, sports, and water-sprinkler recreation. o Distributed lunches to young children. o Filled out appropriate paper work for lunch deliveries.