What groups of methods for creation of anaerobic conditions for anaerobic bacteria cultivation do you know? A. mechanical B. physical C. chemical D. biological E. all answers are correct ANSWER: E It is necessary to verify the hemolytic properties of bacteria. What nutrient media will you recommend? A. simple MPA B. simple MPB C. Endo’s medium D. blood MPA E. serum MPA ANSWER: D Choose obligate aerobes among these microorganisms: A. Neisseria gonorrhoeae, Streptococcus pneumoniae B. Clostridium tetani, Clostridium botulini C. Shigella dysenteriae, Salmonella typhi D. Bacillus anthracis, Brucella melitensis E. Mycobacterium tuberculosis, Micrococcus spp. ANSWER: E Choose obligate anaerobes among these microbes: A. Bacillus anthracis B. Mycobacterium tuberculosis C. Bacteroides spp. D. Rickettsia spp. E. Corynebacterium spp. ANSWER: C Indicate among these microbes obligate anaerobic bacteria: A. Staphylococcus spp., Streptococcus spp. B. Clostridium perfringens, Bacteroides spp. C. Escherichia coli, Salmonella typhi D. Bacillus anthracis, Brucella abortus E. Mycobacterium tuberculosis, Corynebacterium diphtheriae ANSWER: B To the universal nutrient media belong: A. serum MPA and serum MPB. B. Endo’s medium and Ploskirev’s medium C. MPA and MPB D. Blood MPA and TCBS medium E. Yolk salt agar and ascitic agar ANSWER: C Such groups of bacteria are existed according to their respiration: A. capneic B. microaerophils C. anaerobes D. aerobes E. all of them ANSWER: E It is necessary to make biochemical identification and examine saccharolytic properties of bacteria. What media could you offer? A. Veynberg’s medium B. Zeisscler’s medium C. Giss’ media D. Kitt-Tarozzi’s medium E. Milk ANSWER: C Biological identification is carrying out by: A. allergic tests B. inoculation of laboratory animals C. agglutination test D. biochemical reactions E. cultivation on species ANSWER: B Biochemical identification is examination of bacterial species according to their: A. enzymes B. toxins C. allergens D. plasmids E. antigens ANSWER: A For obtaining the isolated colonies of aerobic bacteria tested material is inoculated: A. on solid nutrient media B. in liquid nutrient media C. in laboratory animals D. in chicken embryo E. in cell cultures ANSWER: A Why is it necessary to identify the pure cultures of bacteria? A. for prescribing the treatment B. for revealing the etiological factor illness C. for prophylaxis D. for examination of phages susceptibility E. for examination of antibiotics susceptibility ANSWER: B For what purpose does microbiologist carry out identification of pure cultures? A. For cognition B. For treatment C. For diagnostic D. For prophylactic E. For anamnesis ANSWER: C Cultural properties – there are: A. character of bacterial staining B. character of growth of bacteria on/in nutrient medium C. character of biological features of bacteria D. all answers are correct E. all answers are wrong ANSWER: B Morphological identification is examination of bacterial species according to: A. character of colonies B. morphological signs C. character of staining D. character of motility E. biochemical properties ANSWER: B Serological identification is examination of bacterial species by: A. Bacteria staining B. agglutinating sera C. laboratory animals D. biochemical signs E. character of growth on/in nutrient media ANSWER: B In the process of pure culture identification the most value has: A. Morphological identification B. Biological identification C. Biochemical identification D. Serological E. All answer are correct ANSWER: E For inoculation of microbes by streak’s technique we can use: A. Bacteriological loop B. Spatula C. Bacteriological needle D. All answers are wrong E. All answers are correct ANSWER: A Which of the following vaccines are prepared from live microbes? A. Cholera and dysentery vaccines. B. Measles and Solk polio vaccines. C. Influenza and diphtheria vaccines. D. All of the above. E. BCG and oral polio vaccines ANSWER: E Which of the following vaccine is attenuated? A. Dysentery vaccines. B. Cholera vaccines C. Diphtheria vaccines. D. Solk polio vaccines. E. BCG ANSWER: E Which of the following molecules would be the best antigens? A. lipids B. DNA C. fatty acids D. sugars E. proteins ANSWER: E Which of the following substances are antigens? A. DNA B. carbohydrates. C. lipids D. sugars E. proteins ANSWER: E Which part of the antibody molecule reacts with the antigenic determinants of the antigen? A. H chains B. L chains C. constant parts of the H and L chains D. hinge E. variable parts of the H and L chains ANSWER: E Which part of the immunoglobulin binds with the antigen? A. Fc fragment B. H chains C. L chains D. constant parts of the H and L chains E. variable parts of the H and L chains ANSWER: E Which part of the antibody can binds antigenic determinants of the antigen? A. H chains B. Hinge C. Fc fragment D. L chains E. Fab fragments ANSWER: E Which of the following classes of immunoglobulins can cross the placenta? A. IgA B. IgD C. IgE D. IgM E. IgG ANSWER: E What classes of immunoglobilins contain J chain? A. IgG and IgE B. IgA and IgG C. IgG and IgM D. IgM and IgE E. IgM and IgA ANSWER: E Which of the following classes of antibodies does newborn obtain from mother? A. IgA B. IgD C. IgE D. IgM E. IgG ANSWER: E Plasma cells are: A. mature T cells B. immature macrophages C. mature macrophages D. immature T cells E. antibody-producing cells ANSWER: E Choose among the listed immunoglobulins that contain SP component: A. IgA B. IgM C. IgG1 D. IgE E. SIgA ANSWER: E Choose antibody production cells: A. T cells B. B cells C. T helpers D. Macrophages E. Plasma cells ANSWER: E A toxoid is a(n): A. type of antibody that combines with a toxin B. type of enzyme that destroys toxins C. type of bacterium that resists phagocytosis D. type of virus E. inactivated toxin ANSWER: E For prevent diseases caused by bacterial toxins it necessary uses: A. specific antibodies, B. type of enzyme that destroys toxins C. IgM D. Bacteriophages E. toxoids ANSWER: E Which of the following are antigen presenting cells? A. NK cells and mast cels B. Th and Tk C. Plasma cells D. Cytotoxic cells and nurse cells E. Macrophages and B cells ANSWER: E Which category of hypersensitivity BEST describes hemolytic disease of the newborn caused by Rh incompatibility? A. Atopic or anaphylactic B. Autoimmune C. Delayed D. Immune complex E. Cytotoxic ANSWER: E The role of the macrophage during an antibody response is to: A. make antibody B. lyse virus-infected target cells C. activate cytotoxic T cells. D. produce interleukins E. process antigen and present it ANSWER: E AIDS is caused by a human retrovirus that kills: A. B lymphocytes B. lymphocyte stem cells C. CD8-positive T lymphocytes D. NK cells E. CD4-positive T lymphocytes ANSWER: E Choose secondary immunodeficiency: A. DiGeorge's syndrome B. Wiskott-Aldrich syndrome C. Ataxia-telangiectasia D. Chronic granulomatous disease E. AIDS ANSWER: E A 40X objective and an 10X ocular produce a total magnification of A. 4 B. 50 C. 90 D. 120 E. 400 ANSWER: E Which of the following statements is most correct about the differential Gram stain? A. Crystal violet differentially stains Gram positive cells. B. Gram's iodine differentially stains Gram positive cells. C. Etanol differentially destains Gram negative cells. D. Saffron red differentially stains Gram negative cells. E. Etanol fixes Gram negative cells ANSWER: C Some bacteria are considered pleomorphic.This means: A. they are shaped like bent rods. B. they have a corkscrew shape. C. they do not have just one shape. D. they are not either bacilli or cocci. E. they are not either vibrio or spirochete ANSWER: C The original distinction between the two types of cells, prokaryotic and eukaryotic, was made on the basis of the A. structure of the cell wall B. absence or presence of mitochondria. C. absence or presence of a nuclear membrane. D. absence or presence of ribosomes E. structure of the cell membrane. ANSWER: C What is the function of an illuminator? A. To control the temperature of the specimen B. To keep the specimen moist C. An illuminator is the light source used to observe a specimen under a microscope D. To keep the specimen dry E. No correct answer ANSWER: C Which of the following structures is unique to prokaryotic organisms? A. mitochondria B. ribosomes C. cell wall D. peptidoglycan cell wall E. cell membrane ANSWER: D Cell wall of gram-positive bacteria consist of: A. thin monolayer peptidiglycan B. lipoproteins C. polilayer peptidiglican D. lipopolysaccharides E. outer membrane ANSWER: C What is a smear? A. A smear is a preparation process in which a specimen is spread on a slide. B. A smear is a preparation process in which a specimen is dyed. C. A smear is a process in which a specimen is moved beneath a microscope. D. A smear is a process used to identify a specimen. E. No correct answer ANSWER: A Living, unstained cells and organisms can be observed best using A. fluorescent microscopy B. transmission electron microscopy C. phase contrast microscopy D. Scan. Electron microscopy E. light microscopy ANSWER: C What does Kingdom Prokaryotae include: A. bacteria. B. protozoa. C. fungi. D. viruses E. all of the above. ANSWER: A The most commonly encountered bacteria are roughly spherical. The microbiological term describing this shape is A. coccus B. bacillus C. pleomorphic D. vibrio E. spirochete ANSWER: A Among these bacteria gram-positives there are all, EXCEPT FOR : A. Staphylococci B. Meningococci C. Streptococci D. Clostridium botulini E. Bacillus anthracis ANSWER: B What is a compound microscope? A. A microscope that has one lenses. B. A microscope that has two sets of lenses: an ocular lens and an eye-piece. C. A microscope whose lenses are concave. D. A microscope whose lenses are convex. E. No correct answer ANSWER: B Gram positive cells A. have thick, homogeneous cell walls. B. have large amounts of teichoic acids. C. do not have an outer membrane. D. do not have LPS E. all of the above are true. ANSWER: E At staining by Gram’s method after flood slide with the iodine solution preparation is treated by: A. Fuchsine B. Washing with water C. Crystal violet D. Alcohol E. Sulphuric acid ANSWER: D Spherical bacteria arranged in a chain are known as A. spirochetes B. bacilli C. staphylococci. D. streptobacilli E. streptococci ANSWER: E What is NOT a characteristic of prokaryotic organisms? A. the presence of a cell membrane B. the presence of a nuclear membrane C. "naked" DNA molecule D. the presence of cytoplasm E. No correct answer ANSWER: B The cell wall of Gram-positive bacteria is composed primarily of A. chitin. B. cellulose, C. starch. D. protein. E. peptidoglycan. ANSWER: E What shape is not peculiar for bacteria: A. round or oval B. rod or stick C. icosaedral D. spiral E. No correct answer ANSWER: C What bacteria have the spiral form: A. staphylococcus B. streptococcus C. spirillum D. bacillus E. sarcina ANSWER: C What is a pathogenic microorganism? A. A microorganism that multiplies B. A microorganism that grows in a host C. A microorganism that is small D. A disease-causing microorganism E. No correct answer ANSWER: D Another common bacterial shape is that of a rod, often called A. coccus B. bacillus C. pleomorphic D. vibrio E. spirochete ANSWER: B Cells that do not have a membrane separating their nuclear material from their cytoplasm are called A. heterozygous. B. prokaryotic C. homozygous. D. eukaryotic. E. viruses. ANSWER: B The form of coffee corn is had: A. staphylococcus and streptococcus B. pneumococcus and monococcus C. meningococcus and gonococcus D. peptococcus and peptostreptococcus E. tetracoccus and sarcina ANSWER: C Which of the following bacteria is cell wall deficient? A. Mycoplasma. B. Treponema. C. Slaphylococcus. D. Klebsiella. E. Salmonella ANSWER: A Typical drumstick appearance of bacilli is observed in: A. Clostridium perfringens. B. C. tetani. C. С. botulinum. D. C. histolyticum. E. C. difficilae ANSWER: C Bacterial genus/genera of medical importance which produce endospores is/are: A. Bacillus. B. Bacterium. C. Corynebacterium D. Mycobacterium E. Micrococcus ANSWER: A The construction and use of the compound microscope is attributed to: A. Antony van Leeuwenhoek. B. Louis Pasteur. C. Robert Koch. D. Ferdinand Cohn. E. Paul Ehrlich. ANSWER: A The first techniques of sterilization were introduced by: A. Louis Pasteur. B. Robert Koch. C. Ferdinand Cohn. D. John Needham. E. Gerhardt Domagk. ANSWER: A Who did discovere bacillus of tuberculosis? A. Hansen. B. Loeffler. C. Robert Koch. D. Bruce. E. Pasteur ANSWER: C Who did coin the term vaccine? A. Edward Jenner. B. Kitasato. C. Ehrlich. D. Louis Pasteur, E. Robert Koch. ANSWER: D Who did develop rabies vaccine for the first time in 1885? A. Louis Pasteur. B. Semple. C. Edward Jenner D. Paul Ehrlich. E. Wasserman. ANSWER: A Morpholohgical classification of spherical bacteria: A. monococci and diplococci B. streptococci and staphylococci C. tetracocci and sarcina D. Correct all E. No correct answer ANSWER: D Morphological classification of rod-shaped bacteria: A. monobacilli and monobacteria B. diplobacteria and diplobacilli C. streptobacteria and streptobacilli D. Correct all E. No correct answer ANSWER: D What microbes do belong to coccal forms? A. Escherichia coli B. Staphylococcus aureus C. Mycobacterium tuberculosis D. Yersinia pestis E. None of above ANSWER: B What microbes do belong to coccal forms? A. Salmonella B. Leptospira C. Yersinia D. Clostridia E. Streptococcus ANSWER: E What microbes do belong to coccal forms? A. Bordetella B. Treponema C. Micrococcus D. Vibrio E. Shigella ANSWER: C What microbes do belong to coccal forms? A. Neisseria B. Borrelia C. Corynebacteria D. Proteus E. Spirilla ANSWER: A What microbes do belong to spiral forms? A. Neisseria B. Borrelia C. Corynebacteria D. Proteus E. Spirilla ANSWER: E What microbes do belong to spiral forms? A. Bordetella B. Treponema C. Micrococcus D. Sarcina E. Shigella ANSWER: B What microbes do belong to spiral forms? A. Salmonella B. Leptospira C. Yersinia D. Clostridia E. Streptococcus ANSWER: B What microbes do belong to spiral forms? A. Bordetella B. Clostridia C. Micrococcus D. Vibrio E. Shigella ANSWER: D Mark rod-shaped bacteria: A. Vibrio B. Treponema C. Borrelia D. Escherichia coli E. Staphylococcus ANSWER: D Mark rod-shaped bacteria: A. Sarcina B. Spirilla C. Mycobacterium D. Streptococcus E. Neisseria ANSWER: C Choose rod-shaped bacteria: A. Clostridium B. Meningococcus C. Vibrio D. Borrelia E. Sarcina ANSWER: A Show diplococci: A. Vibrio B. Treponema C. Neisseria D. Borrelia E. Clostridium ANSWER: C Indicate gram-positive bacteria. A. Escherichia coli B. Neisseria meningitidis C. Staphylococcus aureus D. Vibrio cholerae E. Treponema palloidum ANSWER: C Indicate gram-positive bacteria. A. Streptococcus B. Vibrio C. Borrelia D. Neisseria E. Salmonella ANSWER: A Indicate gram-positive bacteria. A. Salmonella and Shigella B. Micrococcus and Tetracoccus C. Vibrio and Spirocheta D. Borrelia and Leptospira E. Neisseria and Treponema ANSWER: B Indicate gram-positive bacteria. A. Neisseria and Treponema B. Vibrio and Spirocheta C. Borrelia and Leptospira D. Salmonella and Shigella E. Bacillus and Clostridium ANSWER: E Indicate gram-negative bacteria. A. Neisseria and Treponema B. Salmonella and Shigella C. Vibrio and Spirocheta D. Correct all E. No correct answer ANSWER: D Indicate gram-negative bacteria. A. Clostridium botulinum B. Bacillus anthracis C. Neisseria meningitidis D. Micrococcus luteus E. Staphylococcus epidermidis ANSWER: C Primary lymphoid organ/s are: A. thymus B. bursa of Fabricius. C. bone marrow. D. all of the above are correct E. E all of the above are false ANSWER: D T-killer cells are important in controlling A. virus infections B. allergy C. autoimmunity D. antibodies synthesis E. all of these ANSWER: A All of the following are organs of immune system, except: A. GALT B. spleen C. lymph nodes D. thyroid gland E. tonsil gland ANSWER: D Why does lysozyme destroy bacteria cells? A. because inhibits protein synthesis B. because activates lysosome’s enzymes C. because inhibits mRNA D. because attacks of peptidoglycan E. because stimulates proteolytic enzymes ANSWER: D Lysozyme: A. aggregates bacteria B. captures free iron C. breaks down the bacterial cell wall D. enhances phagocytosis E. is antiviral ANSWER: C Stem cells arise from: A. yolk sac. B. foetal liver. C. bone marrow. D. all of the above. E. all of the above are wrong ANSWER: D The classic complement pathway occurs when complement binds with: A. antigen-antibody complex B. factor B C. antigen-IgA complexes D. bacterial capsula E. endotoxin ANSWER: A The most common type of circulating white cell is the: A. neutrophil B. lymphocyte C. basophil D. eosinophil E. monocyte ANSWER: A Choose among the listed central organs of immune system: A. GALT, spleen B. thymus, bone marrow C. lymph nodes, thymus D. thyroid gland, tonsil gland E. bone narrow, spleenbone narrow ANSWER: B Which of the following normal component of serum has antiviral activity? A. complement B. collagenase C. interferon D. lysozyme E. lysosome ANSWER: C Choose a nonspecific chemical barrier to infection: A. NK cells B. unbroken skin C. cilia in trachea D. lysozyme in saliva E. all of these ANSWER: D A. B cells are responsible for which allergies? A. asthma B. anaphylaxis C. serum sickness D. atopy E. all of the above ANSWER: E What is toxoid? A. type of antibody that combines with a toxin, B. type of bacterium that resists phagocytosis. C. type of enzyme that destroys toxins, D. type of virus. E. inactivated toxin. ANSWER: E