Supplemental Materials
Article Title: Impact of predator cues on responses to silver nanoparticles in Daphnia
carinata
Journal Name: Archives of Environmental Contamination and Toxicology
Authors:Guangqiu Qin, YunxiaXiong, Song Tang, Peng Zhao, Jon A. Doering, Shawn
C. Beitel, Markus Hecker, Mao Wang, Hongling Liu, Haoliang Lu, Huamao Du
To whom correspondence should be addressed:
Guangqiu Qin, Ph.D.
Associate Professor
Institute of Toxicology, Guangxi Center for Disease Prevention and Control
NO. 18, Jinzhou Road, Nanning, Guangxi 530028, China
Tel: +86-0771-2518982
Fax: +86-0771-2518696
E-mail:qinguangqiu@hotmail.com
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TableS1. Summary of acute toxicity of PVP coated AgNPs on daphnids.
Species
Age of
Duration
daphnids
Endpoints*
20-50
D. carinata
< 24 hr
48hr
LC50
Effect
concentration
(μg/L)
1.75
PVP coated
PVP coated
PVP coated
PVP coated
PVP coated
PVP coated
10-20
72
15
15
47.8
6.3-8.4
D. magna
D. magna
D. pulex
D. galeata
D. magna
D. magna
7d
< 24 hr
< 24 hr
< 24 hr
< 24 hr
< 24 hr
48hr
48hr
48hr
48hr
48 hr
48hr
LC50
LC50
EC50
EC50
LC50
EC50
PVP coated
PVP coated
PVP coated
Cue + PVP coated
Cue + PVP coated
15
35-50
20-50
20-50
20-50
D. magna
D. magna
D. carinata
D. carinata
D. carinata
< 24 hr
10 d
< 24 hr
< 24 hr
< 24 hr
48hr
24hr
24hr
24hr
48hr
EC50
LC50
LC50
LC50
LC50
AgNPs Exposure
NP
size(nm)
PVP coated
soluble Ag %
References
7.3%
This study
2
4.79
8.95
13.9
14.81
54
0.4%
N.A.
< 2%
< 2%
10.3%
N.A.
Zhao and Wang, 2012
Silva, et al., 2014
Völker, et al. 2013
Völker, et al. 2013
Newton, et al., 2013
Blinova, et al., 2013
121
10.6
3.56
9.93
3.61
< 2%
11.3%
7.3%
7.3%
7.3%
Völker, et al., 2013
Poynton, et al., 2012
This study
This study
This study
* LC = lethal concentration; EC = effective concentration for immobilization; N.A. = not available.
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References:
Blinova I, Niskanen J, Kajankari P, Kanarbik L, Kakinen A, Tenhu H, Penttinen OP,
Kahru A(2013) Toxicity of two types of silver nanoparticles to aquatic
crustaceans Daphnia magna and Thamnocephalus platyurus. Environ Sci
Pollut Res Int 20: 3456-3463.
Newton KM, Puppala HL, Kitchens CL, Colvin VL, Klaine SJ (2013) Silver
nanoparticle toxicity to Daphnia magna is a function of dissolved silver
concentration. Environ Toxicol Chem 32: 2356-2364.
Poynton HC, Lazorchak JM, Impellitteri CA, Blalock BJ, Rogers K, Allen HJ,
Loguinov A, Heckrnan JL, Govindasmawy S (2012) Toxicogenomic responses
of nanotoxicity in Daphnia magnaexposed to silver nitrate and coated silver
nanoparticles. Environ Sci Technol 46: 6288-6296.
Silva T, Pokhrel LR, DubeyB, Tolaymat TM, Maier KJ, Liu XF (2014) Particle size,
surface charge and concentration dependent ecotoxicity of three organo-coated
silver nanoparticles: comparison between general linear model-predicted and
observed toxicity. Sci Total Environ 468: 968-976.
Völker C, Boedicker C, Daubenthaler J, Oetken M, Oehlmann J (2013) Comparative
toxicity assessment of nanosilver on three Daphnia species in acute, chronic
and multi-generation experiments. PLoS One 8: e75026.
Zhao CM, Wang WX (2012) Importance of surface coatings and soluble silver in
silver nanoparticles toxicity to Daphnia magna. Nanotoxicology 6: 361-370.
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