Victoria McLain
Briana Price
Austin Graves
Chris Forcinito
Gideon Whaley
Josh Taylor
Sam Holden
Can Daphnia Magna and
Vibrio Fischeri be used in
a quick and simple
nitrogen test?
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4 100 ml graduated
cylinders
4 storage bottles
4 flasks
Sodium Nitrate (s)
Sharpie
Centigram Balance
Weigh boats
Tape
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Fill graduated cylinders to 100 ml of
dH2O
Weigh 10 g of Sodium Nitrate
Add the 100 ml of dH2O into flask
Add 10 g of Sodium Nitrate to flask
Mix well
Put in storage container and label
concentration
Repeat procedure with varying
weights of Sodium Nitrate
depending on wanted concentration
Percent solution= (dry mass in
grams/ volume in mL) *100
Day of Death for Daphnia (y)
Solution
Number (x)
100
parts/million
14
Day of Death for
Daphnia (y)
12
10
12
Number of
Days to death
of sample
population (x)
8
6
1000
parts/million
6
Day of Death for
Daphnia (y)
4
2
10000
parts/million
4
100000
parts/million
2
0
Parts per million (y)
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Over the course of this experiment, we did
indeed test the Daphnia. However, we do not
believe that the data that we collected is
exclusive enough to answer the question that
was posed.
We only tested a few sets of Daphnia.
 We were not able to test them in increments of
minutes, but days.
 The Daphnia took a substantially long amount of
time to produce evidence, or change.
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Slants containing bacteria.
Test tubes containing broth.
Heat source.
Incubator.
Plastic rods.
Petri Dishes.
Small paper discs.
We have concluded that, as per our original
research question about the efficiency of using
Vibrio Fischeri for nitrogen testing, Vibrio
Fischeri is not an expeditious tester.
We found the following issues:
 Growth time
 Obscure effects
 Saltwater vs. Freshwater
 Fragile
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http://upload.wikimedia.org/wikipedia/com
mons/c/c0/Daphnia_magna.png
http://slowmuse.files.wordpress.com/2009/0
2/daphnia-15551.jpg
https://wildco.com/images/pctr/4120A10.jpg
http://dl.clackamas.cc.or.us/ch10401b/images/104vol01.jpg