John James Smith
johnjsmith@gmail.com | 2234 Benjamin Franklin Rd | Philadelphia, PA 19130 | 123-456-7890
__________________________________________________________________________________________
EDUCATION
University of Pennsylvania
 Master of Science in Engineering degree program in Nanotechnology
Philadelphia, PA
Expected graduate
Spring 2016
King University
 B.S. Biochemistry with honors. GPA: 3.62/4
NY, NY
2007-2011
WORK EXPERIENCE
University of Pennsylvania
Philadelphia
Biophysical Engineering & NanoBio-Polymers Lab, Prof. Dennis Discher laboratory
Feb2015-present
 Research Assistant
Working in projects, including laboratory technical skills and critical thinking of quantifying and analyzing data
for publication quality
o Tissue culture
 Preparing sterile media and solutions.
 Culturing and maintain cell lines, which requires good sterile technique and ability to determine cell
counts and cell viability using microscope and cell counting devices
 Plating different cell types on collagen coated gels and matrigel to study the biomechanical activity of
plated cells
o Sonication
 Lysing cell by disrupt cell membrane to perform experiments on proteins of interest
o Electrophoresis
o Running both Agrose/SDS-PAGE
o Immunoblotting
 Immunofluorescence blotting and imaging using immune flourescent microscope
 Western blot for quantifying protein and evaluating effect of drugs on protein expression
o Extracting chick hearts and monitoring the effect of drugs on beating activity.
o Gelation and coverslips prepration
 Using RCA to clean glass cover slips, including removal of organic and ionic contaminants and thin
oxide layer
 Preparing allyltrichlorosilane (ATCS) glass coverslips to provide hydrophobicity to the surface
 Collagen gel with different stiffness coated coverslips to mimic cellular level environment for cell
culture
 Matrigel coated coverslips for culturing Cardiomyocytes
King Fahd Medical Research Center (KFMRC)
Medical Biophysics Unit
 Used FTIR to identify unknown materials and to determine
the quality or consistency of a liver sample.
 Worked on Differential Scanning Calorimetry (DSC) to investigate
thermal transitions of Glucose.
Molecular Biology Unit
 Trained on PCR , polyacrylamide and Agarose Gel electrophoresis and result
interpretation.
Jeddah, K.A.S
July 2009-Sep 2009
Animal House Unit
 Participated in Rabbit Mastectomy, it is done to collect and analyze the Sarcoma.
Immunology Unit
 Used ELISA to identify Hepatitis and Flow Cytometry to detect infected leukocytes.
Smith Hospital
Jeddah, K.A.S
July 2008-Sep2008
Clinical Biochemistry
 Handled specimens correctly to ensure the validity of results then centrifuged samples
and investigated them
Hematology
 Used Cell-Dyn for CBC and visited inpatients to do PTT and PT as lab duties
Serology
 Performed hormones test in patients’ serum for diagnostic purposes.
Blood Bank
 Tested blood groups and their compatibilities by using Rhesus Kit and separated
platelets by performing Plateletpheresis.
_____________________________________________________________________________________
PROFESSIONAL DEVELOPMENT
WORKSHOPS
Vital Role of Preclinical studies in Safety and Effectiveness of Drugs
 Participated as a trainee in drug investigations and studied their physiological
and psychological effects on rats for approval of drugs by Saudi FDA.
Risk management
 Disposed biohazardous materials and chemical material.
SEMINAR

Presenter “Crime Of The Century: Who Killed Lynda In 1983 And Dawn In
1986?”( First application of DNA fingerprint in Forensic science )
SKILLS
Computer
 Microsoft Office (Word, Excel, Access, PowerPoint).
 Bioinformatics Programs ( Symyx Draw, Rasmoll,
Biochem draw, Image j, Graph Pad Prism) and SPSS.
 Matlab( Solving differential equations)
Language
 Arabic (native)
 English (fluent)
NY, NY
Oct, 2009
April, 2009
NY, NY
May, 2010