John James Smith johnjsmith@gmail.com | 2234 Benjamin Franklin Rd | Philadelphia, PA 19130 | 123-456-7890 __________________________________________________________________________________________ EDUCATION University of Pennsylvania Master of Science in Engineering degree program in Nanotechnology Philadelphia, PA Expected graduate Spring 2016 King University B.S. Biochemistry with honors. GPA: 3.62/4 NY, NY 2007-2011 WORK EXPERIENCE University of Pennsylvania Philadelphia Biophysical Engineering & NanoBio-Polymers Lab, Prof. Dennis Discher laboratory Feb2015-present Research Assistant Working in projects, including laboratory technical skills and critical thinking of quantifying and analyzing data for publication quality o Tissue culture Preparing sterile media and solutions. Culturing and maintain cell lines, which requires good sterile technique and ability to determine cell counts and cell viability using microscope and cell counting devices Plating different cell types on collagen coated gels and matrigel to study the biomechanical activity of plated cells o Sonication Lysing cell by disrupt cell membrane to perform experiments on proteins of interest o Electrophoresis o Running both Agrose/SDS-PAGE o Immunoblotting Immunofluorescence blotting and imaging using immune flourescent microscope Western blot for quantifying protein and evaluating effect of drugs on protein expression o Extracting chick hearts and monitoring the effect of drugs on beating activity. o Gelation and coverslips prepration Using RCA to clean glass cover slips, including removal of organic and ionic contaminants and thin oxide layer Preparing allyltrichlorosilane (ATCS) glass coverslips to provide hydrophobicity to the surface Collagen gel with different stiffness coated coverslips to mimic cellular level environment for cell culture Matrigel coated coverslips for culturing Cardiomyocytes King Fahd Medical Research Center (KFMRC) Medical Biophysics Unit Used FTIR to identify unknown materials and to determine the quality or consistency of a liver sample. Worked on Differential Scanning Calorimetry (DSC) to investigate thermal transitions of Glucose. Molecular Biology Unit Trained on PCR , polyacrylamide and Agarose Gel electrophoresis and result interpretation. Jeddah, K.A.S July 2009-Sep 2009 Animal House Unit Participated in Rabbit Mastectomy, it is done to collect and analyze the Sarcoma. Immunology Unit Used ELISA to identify Hepatitis and Flow Cytometry to detect infected leukocytes. Smith Hospital Jeddah, K.A.S July 2008-Sep2008 Clinical Biochemistry Handled specimens correctly to ensure the validity of results then centrifuged samples and investigated them Hematology Used Cell-Dyn for CBC and visited inpatients to do PTT and PT as lab duties Serology Performed hormones test in patients’ serum for diagnostic purposes. Blood Bank Tested blood groups and their compatibilities by using Rhesus Kit and separated platelets by performing Plateletpheresis. _____________________________________________________________________________________ PROFESSIONAL DEVELOPMENT WORKSHOPS Vital Role of Preclinical studies in Safety and Effectiveness of Drugs Participated as a trainee in drug investigations and studied their physiological and psychological effects on rats for approval of drugs by Saudi FDA. Risk management Disposed biohazardous materials and chemical material. SEMINAR Presenter “Crime Of The Century: Who Killed Lynda In 1983 And Dawn In 1986?”( First application of DNA fingerprint in Forensic science ) SKILLS Computer Microsoft Office (Word, Excel, Access, PowerPoint). Bioinformatics Programs ( Symyx Draw, Rasmoll, Biochem draw, Image j, Graph Pad Prism) and SPSS. Matlab( Solving differential equations) Language Arabic (native) English (fluent) NY, NY Oct, 2009 April, 2009 NY, NY May, 2010