Current applications of wheat and wheat-alien precise genetic stocks (by Elena K. Khlestkina)
Supplementary Table 1. Examples of recent applications of wheat precise genetic stocks and wheat-alien lines.
Lines (reference)
Application
Reference
Nullitetrasomic and/or ditelosomic lines
Nullitetrasomic (Sears, 1953) and/or ditelosomic
(Sears, 1944, 1946) lines of bread wheat ‘Chinese
Spring’ (‘CS’)1
Purifying individual chromosome arms of wheat using
flow sorting, production of chromosome arm-specific
BAC libraries and obtaining chromosome arm-specific
sequences
Chromosomal location of wheat DNA-markers
Chromosomal location of wheat ESTs
Chromosomal location of wheat genic sequences
Chromosomal location of wheat genes on proteomic
platform
Identification of specific homoeologues
Studying homoeologous gene expression in wheat
Gill et al. 1999; Kubaláková 2002;
Guo et al. 2006; Janda et al. 2006;
She et al. 2008; Safár 2010;
Berkman et al. 2011, 2012; Wicker
et al. 2011; Hernandez et al. 2012;
Akhunov et al. 2013; Tanaka et al.
2013
Chao et al. 1989; Röder et al. 1995,
1998; Huang et al. 2000; Somers et
al. 2003; Qi et al. 2004; Sourdille et
al. 2004
Qi et al. 2004
Li et al. 1999; Himi and Noda
2004; Boisson et al. 2005; Nomura
et al. 2005; Appleford et al. 2006;
Shitsukawa et al. 2007; Khlestkina
et al. 2008, 2010а
Islam et al. 2002
Shoeva et al. 2014
Bottley et al. 2006
Deletion lines
Deletion lines of bread wheat ‘CS’ (Endo and Gill
1996)1
Physical mapping of wheat DNA-markers
Sourdille et al. 2004
Physical mapping of wheat ESTs
Physical mapping of wheat genic sequences
Qi et al. 2004
Himi and Noda 2004; Boisson et al.
2005; Nomura et al. 2005;
Khlestkina et al. 2008; Shoeva et al.
2014
Islam et al. 2003; Ma et al. 2012
Physical mapping of wheat genes on proteomic
platform
Wheat-alien addition lines
Wheat-rye addition lines T. aestivum CS+Secale
cereale ‘Imperial’
(Driscoll and Sears 1971)1, 2
Purifying individual chromosome arms of rye using
flow sorting, production of chromosome arm-specific
BAC libraries and obtaining chromosome arm-specific
sequences
chromosomal location of rye DNA-markers
Chromosomal location of rye genic sequences
Wheat-barley addition lines T. aestivum CS
+Hordeum vulgare ‘Betzes’ (Islam et al. 1981;
Islam 1983)1, 2
Wheat-Aegilops addition lines
T. aestivum ‘CS’+Aegilops speltoides (Friebe et al.
2000) 1
T. aestivum ‘CS’+Ae. longissima (Friebe et al.
1993) 1
T. aestivum ‘CS’+Ae. searsii (Friebe et al. 1995)1
T. aestivum+Haynaldia villosa (Ying and Chen
2000)3
T. aestivum+Psathyrostachys huashanica (Du et al.
2013a, b)4
Identifying the regulatory activity of certain rye genes
Purifying individual chromosome arms of barley using
flow sorting; production of chromosome arm-specific
BAC libraries and obtaining chromosome arm-specific
sequences
Chromosomal location of barley DNA-markers
Kubaláková et al. 2003; Martis et
al. 2013
Chao et al. 1989; Khlestkina et al.
2004; Bolibok- Brągoszewska et al.
2009; Benito et al. 2010
Khlestkina et al. 2009а; Khlestkina
and Shoeva 2014
Khlestkina, 2010
Suchánková et al. 2006; Mayer et
al. 2011
Chao et al. 1989; Pillen et al. 2000;
Cho et al. 2006; Bilgic et al. 2007
Chromosomal location of Ae. speltoides DNA-markers
Identifying the regulatory activity of certain Aegilops
spp. genes
Dobrovolskaya et al. 2011
Khlestkina 2010
Chromosomal location of H.villosa genic sequences and
identification of chromosome-specific DNA-markers
Identification of chromosome-specific DNA-markers
Zhang et al. 2006; Cao et al. 2011
Du et al. 2013a, b
1
Supplementary Table 1. (continuation)
Lines (reference)
Application
Reference
Wheat-alien chromosome substitution lines
Wheat-rye substitution line T. aestivum
‘Saratovskaya 29’(S.cereale ‘Onokhoiskaya’)
(Silkova et al. 2006)5
Wheat-barley substitution line T. aestivum
‘Pyrothrix 28’(H. marinum)
(Trubacheeva et al. 2008)5
Wheat-Aegilops substitution line T. aestivum
‘CS’(Ae. tauschii) (McFadden and Sears 1947)2
Assessment of activity of certain non-wheat genes at
wheat background
Khlestkina et al. 2009а
Khlestkina 2010
Intervarietal single chromosome substitution lines
‘CS’(‘Hope’) (Kuspira and Unrau 1958)1
‘Saratovskaya 29’(Yanetzkis Probat) (Gaidalenok
et al. 2005)5
Determining role of the genes underpinning
pigmentation of different parts of wheat plant in
flavonoid biosynthesis regulatory network; studying
homoeologous gene expression in wheat
Himi et al. 2005; Khlestkina et al.
2008, 2010b; Khlestkina 2010
Single chromosome recombinant lines (SCRLs)
T. aestivum ‘CS’(‘Cheyenne’ 5B)/‘CS’, ‘Hobbit
Sib’(‘CS’ 5BL)/ ‘Hobbit Sib’ (Tóth et al. 2003)6
T. aestivum ‘Favorit’(‘F26-70’ 7B)/ ‘Favorit’
(Giura and Ittu 1986; Khlestkina et al. 2009b)2
T. aestivum ‘CS’(T. spelta 7D)/‘CS’
(Simon et al. 2005, 2010)2
Detection of QTLs for flowering time and frost
resistance
Mapping genes for photoperiod response and grain
protein content
Detection of QTLs for septoria resistance
T. aestivum ‘Saratovskaya 29’-T. timopheevii
(Leonova et al. 2004)5
T. aestivum ‘Alcedo’-Ae. markgrafii (Iqbal et al.
2007)2
T. aestivum ‘CS’-Ae. tauschii (Pestsova et al.
2006)2
Determining precise positions of the genes
underpinning traits or encoding enzymes
Tóth et al. 2003
Khlestkina et al. 2009b
Simón et al. 2010
Introgression lines (ILs)
T. aestivum-H. villosa T6VS-6AL (Chen et al.
1995)3
Determining role of the genes underpinning
pigmentation of different parts of wheat plant in
flavonoid biosynthesis regulatory network
Identification and precise location of the candidate gene
for the powdery mildew resistance gene Pm21
Leonova et al. 2008; Tereshchenko,
2012
Iqbal et al. 2007
Pestsova et al. 2006
Khlestkina et al. 2008; Khlestkina,
2010
Cao et al. 2011
T. aestivum ‘Saratovskaya 29’-T. timopheevii/ T.
aestivum ‘Rodina’- Ae.speltoides
(Tereshchenko et al. 2012а)5
Finding novel genes by their complementary effect in
the IL
Tereshchenko et al. 2012а
NILs of ‘Saratovskaya 29’ (Arbuzova et al. 1998)
and ‘Novosibirskaya 67’ (Koval, 1997)2, 5
Determining precise positions of the genes
underpinning pigmentation of different parts of wheat
plant
Determining role of the genes underpinning
pigmentation of different parts of wheat plant in the
flavonoid biosynthesis regulatory network
Khlestkina et al. 2000;
Tereshchenko et al. 2012b
NILs of ‘Bezostaya 1’ (Efremova et al. 2011)5
NILs of ‘Triple Dirk’ (Pugsley 1971, 1972)7
NILs of ‘Century’ (Carver et al. 1993)8
NILs of ‘Sumai’/‘Stoa’ (Pumphrey et al. 2007)9
NILs of ‘Thatcher’ (Dyck and Samborski 1968)10
Establishing functional differences between Vrn
(vernalization sensitivity) alleles and homoeoalleles
Establishing gene networks involved into biotic and
abiotic stress resistance by transcriptome profiling of
the NILs
NILs of ‘98B69*L47’ and ‘BW30’
(Hamzehzarghani et al. 2008; Gunnaiah et al.
2012)11
Studying metabolic pathways/ gene networks involved
into fusarium head blight resistance by metabolome
and/or proteome profiling of the NILs
Shcherban et al. 2013
Loukoianov et al. 2005
Houde and Diallo 2008
Jia et al. 2009
Hulbert et al. 2007; Bolton et al.
2008; Manickavelu 2010
Hamzehzarghani et al. 2008;
Gunnaiah et al. 2012
Near-isogenic lines (NILs)
Himi et al. 2005; Khlestkina 2010;
Tereshchenko et al. 2013
1
Wheat Genetics Resource Center of the Kansas State University (Manhattan, USA)
Genbank of the Leibniz Institute for Plant Genetics and Crop Plant Research (Gatersleben, Germany)
3
Cytogenetics Institute of Nanjing Agricultural University (Nanjing, China)
4
Genetic Engineering for Plant Breeding, College of Agronomy, Northwest A&F University (Shaanxi, China)
5
Institute of Cytology and Genetics SB RAS (Novosibirsk, Russia)
6
John Innes Centre (Norwich, UK)
7
Washington State University (Pullman, USA)
8
Oklahoma State University (Stillwater, USA)
9
University of Minnesota (St. Paul, USA)
10
Kihara Institute for Biological Research, Yokohama City University (Yokohama, Japan)
11
McGill University (Quebec, Canada)
2
2