Detection of Some Resistance Genes (Bt-5, Bt-8, Bt-10, Bt-11 and Bt-12)
against Common Bunt in Ten Wheat Varieties Using Molecular Markers.
Nurcan GÜMÜŞ1 , Ismail Poyraz1, İlham ERÖZ POYRAZ2,
1
Department of Molecular Biology and Genetic, Faculty of Art & Science, Bilecik Seyh Edebali University, 11210 Bilecik,
Turkey.
2
Faculty of Pharmacy, Anadolu University, 26470, Eskişehir, Turkey.
ABSTRACT
Common bunt (Tilletia sp.) is a very dangerous seed-borne disease in wheat and may
cause serious economic losses. Wheat cultivars including resistance genes are used as
an alternative fight method instead of chemical fungicides against common bunt
disease. These resistance genes in wheat are called as bt genes. Until today, a little
number of bt genes in wheat has been detected by some molecular markers. There
aren’t yet developed molecular markers for detection of all bt genes in wheat. In this
study, detection of some bt genes (bt-5, bt-8, bt-10, bt-11 and bt-12) was carried in
registered eighteen wheat varieties (Sertak 52, Bolal 2973, Demir, Kutluk,
Harmankaya 99, Pehlivan, Tosun Bey, 4-11, Sönmez 01, Bezostaja-1) using PCRbased molecular markers (Microsatellite and RAPD). PCR-amplified fragments were
separated on 1.3% agarose gel containing ethidium bromide (0.5 μg/ml). Gels were
visualized under UV light and digitally photographed. The obtained DNA bands were
scored as present or absent for detection of bt genes. For comparison, the virulence
rates of five Tilletia foetida (syn. leaves) isolates against eighteen wheat varieties
were obtained from our field results. We observed that wheat varieties including bt-10
and bt11 genes as Kutluk and 4-11 varieties are more resistant to disease in field. We
showed analysis results for five bt genes in all wheat varieties and resistance rates in
field.
Keywords: Wheat, Bt resistance genes, Common bunt, Molecular markers.