Miss Kanwal Abbasi - Pakistan Council for Science and Technology

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Miss Kanwal Abbasi
3 C 2/16 Nazimabad,
Karachi Pakistan,
Tel No 021-36617808
Cell no,03313303149
E-mail kanwalabbasi@yahoo.com,
Academic Qualification
3 Years B.Sc. honors in Biochemistry
University of Karachi Pakistan in 2001
Stood first in science faculty of university of Karachi with 80.46% marks
M.Sc (Biochemistry) from university of Karachi Pakistan in 2002
(First class first Position with 83.2% marks)
PhD Student department of pathology, University Cambridge United Kingdom.
(Thesis submitted).
Experimental Skills
1) Nucleic acid isolation and manipulations
2) Preparation of plasmid DNA
3) Agarose gel electrophoresis
4) Phenol chloroform extraction
5) Restriction endonuclease digestions
6) DNA ligations
7) Polymerase Chain Reaction (PCR)
8) Gel purifications reaction
9) E.coli transformation procedures
10) Electroporation
11) Yeast transformation
12) FACS analysis Flow cytometry
13) Protein sample preparation
14) SDS-PAGA
15) Western blot analysis
16) Coomassie staining of SDS-PAGE gels
17) Purification of recombinant protein
18) Immunization
19) Affinity purification of Antibodies
20) Immunofluorescence microscopy
21) Electron Microscopy
22) RNA isolation
23) RNA interference
24) Quantitative real time (qRT)-PCR
25) Yeast two-hybrid screening
26) Bacterial two hybrid analysis
27) Antioxidant assay
28) Lactase assay
29) Measurement of DNA vs Histon Ratio
30) Primers or Oligonucleotides synthesis
Experience
I had worked as research associate in Aga Khan medical University for 6
months.
Now I am working as scientific officer in Pakistan council of scientific and
industrial research (Ministry of science and Technology Pakistan).
Hear I established the process of protease (enzyme) production from locally
isolated bacterial strain for commercial use, molecular diagnosis of HCV,
doing PCR and agarose electrophoresis (from 2003 to till date)
(2005-2009), Cambridge United kingdom.
Now working on production of antioxidants, production of lactase enzyme
and anti-inflammatory compounds.
Publications.
1) A Novel Rho-Like Protein TbRHP Is Involved in Spindle Formation and Mitosis in
Trypanosomes. Abbasi K, DuBois KN, Dacks JB, Field MC.
PLoS One › v.6(11); 2011.
2) Alterations in biochemical parameters of hyperlipidemia in coronary artery disease
patients. Siddiqua Jamall, Mohammad Ishaq and Kanwal Abbasi.
J. Biochem. Mol. Biol. 2010; 43(4): 205-207.
3) Hypertriglyceridemia and low HDL-cholesterol are the major lipid abnormalities in
normal Pakistani adults..Mohammad Perwaiz Iqbal, Majid Shafiq, Naseema Mehboobali,
Saleem Perwaiz Iqbal, Kanwal Abbasi, Iqbal Azam, Khawar A Kazmi.
Jan ;20(4):385-91.
ACHIEVEMENTS
Presidential Award winner
The president of Pakistan respected Perwaz Mushraf has been confer me the Presidential
Award of IZAZ-I-SABQAT in recognition of academic destination for the Year 2002
Selected for Commonwealth scholarship tenable in UK for 2004.
Scholarship reference number is (PKCS-2004-297).
Language Test
IELTS with 6.5 bands taken on 13 March 2004
Social worker
Iam member of pakistan most respected N.G.O “FANOS” .
FRIEND OF ANTI-NAROCTICS
Working against the use of drug in Pakistan take part in many welfare programs and
seminars and act in many stage plays against use of drug
Personal
Father Name
Marital Status
Date of birth
Aijaz Hussain Abbasi
Single
18 October 1979
References
Prof., Dr Muhammad Ishaq
Ex-Chairman of department of biochemistry
University of Karachi
Dr. Kaniz fizza
Chief Scientific Officer
PCSIR Complex Karachi
Dr Lubna Iqbal
Principal scientific Officer
PCSIR Complex Karachi
Prof., Dr Mark C.field
Department of Pathology
University of Cambridge UK.
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