Supporting Information Legends
Figure S1. Phenotypes of wild-type C24 and los2-1 mutant plants.
(a) Enolase activity in crude extracts of los2/eno2 mutant plants. Enolase activity was measured in
floral tissues of six-week-old plants of the lines shown. Values with the mean SD calculated from
three independent biological replicates are shown. (b) Enolase activity in crude plant extracts in C24
and los2-1 lines. Enolase activity was determined in floral tissues of six-week old plants. Values are
the mean SD calculated from three independent biological replicates. (c) Phenotypes of 12-day-old
wild-type and homozygous los2-1 seedlings grown on agar plates. (d) Adult phenotypes of five-week
old wild-type and los2-1 plants grown in LD growth conditions.
Figure S2. Lignin, sinapoyl malate, soluble sugar and starch contents in different los2/eno2 mutant
alleles.
(a) Lignin contents in the primary inflorescence stems of six-week-old wild-type and los2-1 plants.
Lignin amounts were determined in aerial tissue of six-week-old plants grown in LD growth
conditions with the AcBr assay. Values are given as percentage of dry cell wall material.
(b) Sinapoyl malate contents in leaves of three-week-old wild-type and los2-1 plants grown in LD
growth conditions.
(c) Soluble sugar content in leaves of three-week-old wild-type and los2-1 grown in LD growth
conditions. Results are shown as the mean ± SD of four biological replicates (each was a pool of five
plants).
(d) Starch content in leaves of three-week-old los2/eno2 knock-out lines. Plants were grown in LD
conditions. Results are shown as the mean ± SD of four biological replicates (each was a pool of five
plants).
Figure S3. Phenotype of four-week-old 35S:LOS2-YFP and 35S:MBP1-YFP mutant plants as compared
to wild-type.
Figure S4. Expression of a LOS2-NES-YFP construct in protoplasts and los2-4 plants.
(a) Transient expression assay of LOS2-YFP and LOS2-NES-YFP in arabidopsis protoplasts and analysis
using fluorescence microscopy. DAPI staining was used to show the nucleus.
(b) Enolase activity in floral tissues of five-week-old plants of the lines shown. Values with the mean
SD calculated from three independent biological replicates are shown.
Table S1. List of primers used in this study.
Supplemental Methods.