Appendix B - IACUC Protocol
IACUC #:
Create and Maintain a Breeding Colony
University of California, Irvine
Institutional Animal Care and Use Committee
Mod #:
For Office Use Only
Protocol #:
Lead Researcher:
Provide the following information for breeding colony maintenance. Place an X in the checkboxes that apply and
complete the application sections below.
Personnel must be included in Sections
F and 9 of the Protocol Application
Section 1: Responsible Personnel
A. Main Person Responsible for the Breeding Colony
Name:
Home Dept:
Phone #:
After-hours Phone #:
UCI Email:
Describe this individual’s training and experience as it relates to colony maintenance and breeding of
animals:
B. Other Research Personnel Involved in Breeding Colony Maintenance
List any other research personnel who will be involved with the maintenance of the breeding colony.
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Name
UCI Email
Training and Experience
Section 2: Breeding Colony General Information
A. Justification and Background Details
Please review the IACUC Policy on the Rodent Breeding Colonies.
1. Provide a scientific justification for establishing and maintaining a breeding colony of animals at UCI:
Cost savings alone is not a valid justification for maintaining a breeding colony.
2. Describe any variations from standard ULAR-provided husbandry, care or housing required by the
breeding colony:
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3. Crossbreeding of Transgenic Rodents
The cross-breeding of two different transgenic rodents or the cross-breeding of a transgenic rodent and
a non-transgenic rodent, with the intent of creating a new strain of transgenic rodent, may require
additional regulatory committee review. Mark all checkboxes that apply.
NOTE: Use of all non-rodent transgenic animals, including their purchase and transfer, requires IBC
review
Both parental strains may be safely housed in ABSL1 containment
Neither parental strain contains more than 50% of the genome of an exogenous eukaryotic virus
from a single family
Neither parental strain includes a transgene that is under the control of a gammaretroviral long
terminal repeat
The transgenic rodent that results from this breeding is not expected to contain more than one half
of an exogenous viral genome from a single family of viruses.
4. Describe the breeding scheme that will be used (monogamous pairs, harem, etc.):
5. Indicate how individual animals will be identified/labeled:
Ear Notch
Ear Tag
Tattoo
Microchip Implant
Cage card only
*Toe Clipping
*Provide scientific justification for the use of toe clipping. Mark the checkbox below to confirm that
requirements will be followed.
Place an X in the checkbox below to indicate researcher acknowledgement:
Toe clipping will be performed only on animals that are less than 7-10 days old. Only
the distal portion (first knuckle) of the second through fifth toe may be removed; removal
of entire digits or any portion of the thumb is not allowed. The Standard Operating
Procedure for Distal Toe Clipping will be followed.
(For more information about toe clipping, see the Transgenic Mouse Facility SOP.)
Other (describe below):
B. Weaning and Genotyping
1. Indicate the age at which animals are weaned:
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Species
Strain
Weaned at:
*For rodents, scientific justification is required for pups that remain with the dam longer than the
customary weaning age (for rodents, this is 21 days). If this will occur, provide scientific justification
below.
(See the Rodent Breeding Colony Policy for more information)
2. Indicate how will samples for genotyping will be collected:
N/A – Animals will not be genotyped
Tail Clip (maximum of 5 mm)
*Tail biopsy of rodents older than 21 days requires general anesthesia. If tail biopsies will occur at
older than 21 days of age, describe the method of anesthesia that will be used:
Other (describe below):
C. Health Monitoring
1. Describe any known or expected phenotypes (behavioral, anatomical or physiological) of animals
produced in the colony.
Behavioral (describe below)
Anatomical (describe below)
Physiological (describe below)
If the phenotype may adversely affect the health and welfare of the animals, describe how animals will
be monitored and treated for pain, distress and discomfort.
2. Indicate whether breeder animals will receive any drugs to enhance reproductive performance (e.g.,
ovulation agents, etc.).
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No
*Yes
Breeder animals will not receive any drugs or agents
Breeder animals will receive drugs or agents (list below)
*List the agent, dosage, and frequency of administration if applicable.
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Agent
Dosage
Frequency
3. Indicate how excess or unused animals in the colony will be euthanized.
Must be listed in Section 8
of Protocol Application
Excess or unused animals are animals with the wrong genotype, retired breeders, animals not needed
for researcher, etc.
*CO2 Overdose with Secondary Physical Method
NOTE: All rodents euthanized with CO2 need to undergo a secondary physical method of
euthanasia to ensure death.
*Indicate secondary physical method:
Other (describe below):
Section 3: Breeding Colony Animal Numbers
Must be listed as Pain Category B in
Section H of the Protocol Application
A. Number of Animals for the Breeding Colony
List the number of animals that will be used for breeding purposes only. This includes founder animals, wildtype strains needed for backcrossing, replacement breeders, cryopreservation, etc.
Note: If this is a renewal application for an existing colony, include the breeder animals that you currently
have in your colony + any additional animals needed for this renewal.
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Species
B. Number of Animals Not Used
Strain
Total Number
Must be listed as Pain Category B in
Section H of the Protocol Application
1. Estimate the approximate number of animals that will be produced in the colony that will not be used for
research or breeding during the 3-year protocol period (e.g., culled littermates, wrong genotype):
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2. Explain why these animals will not be used:
3. Discuss what will be done to minimize this number:
Notes
Investigators are responsible for reporting of ALL animals that are produced in UCI breeding colonies.
The following animals must be reported online:
1. All offspring from breeding colonies, including:
a. Offspring culled from litters
b. Animals retained in the colony to be used as breeders (replacement breeders)
c. Animals used for research purposes (reported in the appropriate USDA pain category)
2. Live-born offspring of animals that are purchased as Pregnant or Dams-With-Litter.
For guidance on how to report online, see Reporting Animal Numbers Online:
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