) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
University of Baghdad
College Name
College of Science
Department
Department of Biology
Full Name as
written in
Passport
GHADAH MOHAMMED SALIH TURKI
e-mail
ghadahalquraishi@yahoo.com
Career
Assistant Lecturer
Lecturer
Master
Thesis Title
Year
Abstract
Assistant Professor
Professor
PhD
Immunological and Bacteriological Study on Patients with Atopic
Dermatitis
2011
A prospective study was conducted to obtain more clarification about the impact of
immunological abnormalities on the expression of atopic dermatitis (AD) among Iraqi
children patients. Fifty four cases of AD (30 males and 24 females ), with ages ranging
between 3 months to 12 years. Patients were grouped as 13 mild AD , 30 moderate AD
and 11 severe AD according to severity and 18 apparently healthy individuals, 18
psoriasis patient, which both included 9 males and 9 females included as controls. They
were studied using different , immunological and hematological tests, in addition
Staphylococcus aureus was isolated and diagnosed. Furthermore, comparisons were
made between the immunological markers in a number of AD conditions.
Atopic dermatitis patients were characterized with increased mean levels of total
IgE,IL-12,IL-13 and IL-18compared to healthy controls and in psoriasis controls, using
the ELISA method for level detection.
Atopic dermatitis as a disease can be introduced by different aspects including acute
and chronic AD, extrinsic and intrinsic AD. The study revealed a significant increase in
the mean total IgE level among acute AD patients compared to chronic AD patients. The
mean serum IL-13 level in acute AD patients was significantly higher than that of
chronic AD patients which may indicate a role of IL-13 in elevation of total IgE levels,
whereas , IL-12 and IL-18 were decreased in acute AD patients ,compared to chronic
AD patients.
Extrinsic and Intrinsic AD was an interesting field to investigate the differences
between these two patterns of AD. Results showed highly elevated total IgE levels in the
extrinsic group of AD compared to normal and even low total IgE levels in the intrinsic
AD group (21 .The mean serum level of IL-13 in extrinsic AD patients was higher than
its level in intrinsic AD patients with a statistical significance .The mean serum IL-12
and IL-18 were significantly elevated in intrinsic AD patients, compared to lower levels
in extrinsic AD patients.
Several autoantibodies have been detected in patients with AD, when ANA were
detected in the AD patients of this study it showed that (7.5% )of them were positive for
ANA compared to (5.5%) in healthy control and (11.1%) in Psoriasis controls with no
) ‫أنموذج ( أ ) الخاص برسائل الماجستير و اطاريح الدكتوراة ( اخر شهادة‬
statistical significance.
The relationship between AD and bacterial colonization was studied. Skin swabs
were taken from AD patients. Samples were cultured on suitable media for isolation and
identification of bacteria. The results showed that Staphylococcus aureus was the most
common isolated bacteria from AD patients with a percentage of (58.3%). Furthermore,
the incidence of S. aureus in severe AD group was higher (69.0%) than in both moderate
and mild AD groups (54.0%, 58.0%) respectively , with a statistical significance at
(P<0.01). These results indicate the role of S.aureus in the severity of AD.
Infection with microorganisms have an impact on immunological response, from
this point of view it was important to detect the cytokine levels and IgE in the cases of
AD patients with S.aureus isolates. Results showed higher total IgE levels in AD
patients with S.aureus isolates than those without isolates , with a statistical significance
of (P<0.01). There was no statistical significance in the IL-18 levels between AD
patients with and without S.aureus isolates, while IL-12 and IL-13 was higher in the first
group with statistical significance at ( P < 0.01).
In conclusion, the association between AD severity and different immunological
markers were assessed. Acute and chronic AD, extrinsic and intrinsic AD ,are phases of
AD disease and are influenced with immunological markers too. In addition, the
persistence of S.aureus was associated with severity of AD disease, and that IL12,13,and 18 were useful markers to assess AD.