Beer`s law lab

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Name:__________________________________________ Date:___________ Due Date:______
Beer’s Law, Cobalt Chloride Lab
Purpose: Using Beer’s Law you will find the concentration of cobalt chloride from the amount
of light absorbed by its solution.
Introduction: A spectrum is a recording of the wavelengths absorbed by a sample. Colored
compounds, such as the pink cobalt chloride used in this experiment absorb in the visible. The
transmittance, %T, is the ratio of the intensity of the incident light (Io) and the intensity of the
emerging light (I), or %T = I / Io. Absorbance, A, is the logarithm of 100 divided by %T: If %T =
50, then, A = log 100/50 = log 2 = 0.30
Beer’s Law
According to Beer’s Law, the absorbance (A) of a solution is proportional both to the molarity of
the solute responsible for the color and to the path length (b) of solution through which the
light passes.
A = a b c where (a) is a coefficient (Beer’s Law constant), the value of which depends on the
wavelength of light, the solvent and the kind of solute used. The path length (b) is the diameter
of the test tube (cm) used to hold the sample. The molarity of the solution is (c). Deviations
from Beer’s Law may be severe for concentrations greater than 0.1 M.
Apparatus
You will be taking a spectrum of cobalt chloride solution using a Spectronic-20. To use this
instrument refer to the operating instructions attached. Be sure to adjust the %T dial for a
100% indication using a blank cuvette for each wavelength.
Procedure and Data Analysis
Part A: Making the dilutions
1. The stock solution is the most concentrated solution to be used in this experiment.
Pour about 50 mL of 0.080 M CoCl2•6H2O into a clean 250 mL beaker. Fill a clean
burette with the contents of the beaker. Note: You will use the stock solution to take a
spectrum and to prepare dilutions for other parts of the experiment.
To verify Beer’s Law you will prepare dilutions of a stock solution to measure their
absorbance at the maximum wavelength.
2. Use two burettes (one for cobalt chloride, another for distilled water). Make sure to
clearly label your burettes.
3. Fill the CoCl2 burette to the 50 ml mark with the stock solution.
4. Fill the water burette to the 50 ml mark with distilled water.
Name:__________________________________________ Date:___________ Due Date:______
5. Deliver 10.0 mL of the stock solution into a clean 50 or 100 mL beaker, then add 5.0
mL of water to dilute the solution (Beaker 1). Mix well with a stirring rod.
6. Repeat step 5 into different beakers for the following amounts:
10.0 mL stock + 10.0 mL water (Beaker 2)
10.0 mL stock + 15.0 mL water (Beaker 3)
10.0 mL stock + 20.0 mL water (Beaker 4)
Calculate the concentrations (molarities) of these diluted solutions and record them in
a data table.
Part B: Taking the Spectrum of the Stock Solution of Cobalt Chloride
1. Fill your test tube to a level of approximately 2 cm with water (the blank) and another
with the stock solution of cobalt chloride (the sample). Set the wavelength dial to 400
nm and measure the % transmittance and absorbance of your sample. Record in the
Data and Results table. Continue to measure the % transmittance every 10 nm between
400 nm and 650 nm. Note: The tubes should be clean, their outer surfaces dry and free
from smudges.
2. Plot the absorbance (y axis) vs. wavelength (x axis) by drawing a smooth line through
the points. This is the visible spectrum of the stock solution of cobalt chloride. Submit
the plot with your Data and Results. Record the wavelength at which the maximum
absorbance is observed.
Part C: Measuring Absorbance of Diluted Solutions of Cobalt Chloride
1. Measure the % transmittance and absorbance of each diluted solution you created in
Part A. Note: Rinse the cuvette with each new solution before each reading. Make sure
you use a blank before testing each sample. Start with your most dilute solution and
work your way up to the most concentrated (stock solution). Record your
concentration versus transmittance data in your lab notebook.
2. Plot the absorbance (y axis) vs. the concentration (x axis). The plot should produce a
straight line. Submit the plot with your Data and Results.
3. Find (b) the path length by measuring the diameter of the test tube.
4. Find (a), the Beer's Law constant, you must first find the slope of your best fit line.
Part D: Determining the Concentration of an Unknown Cobalt Chloride Solution
1. Measure the % transmittance and absorbance of the unknown sample at the peak
wavelength used for the known samples, and use the value of (a) and (b) calculated in
Part C to find the concentration of your unknown sample.
Name:__________________________________________ Date:___________ Due Date:______
Conclusion Questions:
1. Would you be able to use your data to find the concentration of a 10.00 M solution of
cobalt chloride? Why or why not?
2. A student is instructed to determine the concentration of a solution of CoCl 2 based on
absorption of light (spectrometric/colorimetric method). The student is provided with a
0.10 M solution of CoCl2 with which to prepare standard solutions with concentrations
of 0.020 M, 0.040 M, 0.060 M, and 0.080 M.
(a) Describe the procedure for diluting the 0.10 M solution to a concentration of 0.020 M
using distilled water, a 100 mL volumetric flask, and a pipet or buret. Include specific
amounts where appropriate.
The student takes the 0.10 M solution and determines the percent transmittance and the
absorbance at various wavelengths. The two graphs below represent the data.
(b) Identify the optimum wavelength for the analysis._____________________
The student measures the absorbance of the 0.020 M, 0.040 M, 0.060 M, 0.080 M, and 0.10 M
solutions. The data are plotted below.
(c) The absorbance of the unknown
solution is 0.275. What is the concentration
of the solution?
(d)
Beer's Law is an expression
that includes three factors that determine
the amount of light that passes through a
solution. Identify two of these factors.
Name:__________________________________________ Date:___________ Due Date:______
(e)Before inserting the sample of CoCl2 into the Spec-20, it is necessary to calibrate the
instrument to read 100% T with a blank. What is the blank in this lab and why is this
step necessary?
(f) Why is this method of determining the concentration of CoCl2 solution appropriate,
whereas using the same method for measuring the concentration of NaCl solution
would not be appropriate?
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