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Work Request -- COBRE-PSL Protein Structure Laboratory
Please provide the following information. This information is intended to ensure that we know exactly what you
want us to do. The cost estimate we provide will be based on this information.
 Type directly onto to this Microsoft Word document; it will expand as you type, but please be concise, and
please use 10 point Arial font for readability.
 Save the document as “last name_first name_PSL_Work_Request_Form” (without quotes) and submit it
electronically to Dr. Scott Lovell, Director, COBRE Protein Structure Lab <swlovell@ku.edu>.
1. Submission date:
2. Submitter Information (PI / Faculty / equivalent)
Name
Phone
Dep't.
E-mail
3. Indicate Location: ( )KU ( )KUMC ( )KSU ( )WSU ( )Other_______________________________
4. Laboratory Contact (research assistant/associate)
Name
Phone
Dep't.
E-mail
5. Description of work requested. Tell us exactly what you want us to provide (i.e. define the "deliverable" or
"product" you expect to receive from this work request. Examples might include: consultation, training, protein
crystallization, cocrystallization with ligands, ligand soaking of crystals, X-ray data collection and/or structure
solution.
6. Methods.
Are there any specific methods that you recommend or request? Please provide relevant background and/or
specific literature references (attach PDF copies if available). Mention special precautions, if any.
7. Will personnel from your lab be involved in any of the work being requested? If so, please explain how:
8. Materials to be provided by Requestor. What will you provide - protein, crystallization solutions, plates,
ligands etc.? Explain if any of these items are "precious" (extremely costly or in very limited supply).
NOTE: It is recommended that protein samples are concentrated between 8 to 12 mg/mL. The standard
screening protocol includes six screens (544 drops) incubated at a single temperature and would require 300 L
of the concentrated protein sample. If this sample volume is not available the minimum volume necessary for
crystallization screening is 50 L of the concentrated protein sample. This would provide enough of the sample
for a single 96-condition crystallization screen. Protein purity is crucial for crystallization experiments. If
available, please submit a picture of the SDS-PAGE gel for your final purified sample along with this work request
form.
9. Protein Information: Please give the name and exact amino acid sequence of protein sample used for
crystallization: (List ALL residues including those resulting from cloning and/or purification tags.).
9.a. Protein Name:
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9.b.Complete Amino Acid Sequence for your sample:
9c. Protein Purification Method. Please describe the methods used for purification of the submitted sample.
Include any chemical components such as salts, buffers, reducing agents, etc. that were utilized in the purification
steps from initial lysis to delivery of the final purified sample. This is important as sometimes these materials
show up in the crystal structure.
9.d. Protein Concentration (mg/mL):
9.e. Protein Storage Buffer, pH, concentration:
9.f. Sample Volume (L):
9.g. Additional Information: (i.e., stability issues, disulfides, multimeric state, etc.)
Click here to enter any additional information regarding your sample.
9.h. Structural Information: If requesting a crystal structure, is the structure of your protein or a homologous
protein known? If yes please provide any relevant Protein Databank accession codes.
Yes ☐ No ☐
If yes, enter PDB Codes here:
10. Ligand Information: Please provide the following information for any small molecule compounds that will be
used for cocrystallization and/or crystal soaking experiments. If a solution of a particular small molecule is
supplied, please provide the concentration and solvent information if applicable.
Ligand Name / ID
Molecular Weight (g/mol)
Concentration (mM)
Solvent:
11. Reason for this request. How will you use the results? Please check one.
( ) Test a preliminary idea for a potential future project.
( ) Obtain preliminary data to support a new grant application in preparation.
( ) Increase/accelerate the productivity of a funded project in my lab.
( ) Other (please explain briefly below).
12. Please indicate ( X ) the source of support for the work being requested.
( ) A. This project is supported by Regular COBRE-PSF Pilot Project Award.
( ) B. Client requests a COBRE-PSF subsidy coupon (then check either B1 or B2 below).
NOTE: Coupons MUST be arranged and agreed upon in advance of the project.
( ) B1. Client has ≥ 1 major active grant (e.g., NIH R01, NSF, or similar).
( ) B2. Client has limited or negligible resources (e.g. small grants only, no-cost extension, etc.)
( ) C. Other non-grant support.
13. If either B or C is checked above, please provide ALL requested billing information below (REQUIRED).
Address Invoice(s) to (name)
area code and phone #
Email address
Institution / Department
Mailing address / zip
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funding agency
grant # or P.O.#
grant ending date
-----------------------------TERMS AND CONDITIONS ----------------------------1. Timing. Requests are generally processed in the order received. If there is any "special" urgency or timing
consideration, such as a looming grant application deadline, please explain here in detail.
2. Authorship and acknowledgement policies. These policies are available from each of the Core Lab
websites at http://psf.cobre.ku.edu. Submission of this work request indicates the client's awareness of and
agreement to abide by these policies, and specifically the following:
2.1) Proper acknowledgement of the specific COBRE Core Lab and the COBRE grant P30GM110761 on all
publications reporting work done using COBRE facilities, AND
2.2) Possible authorship rights of Core Lab personnel arising from the work performed. It is our policy that
Core Lab personnel whose work enables a project to succeed deserve recognition as co-authors of the work.
2.3) For questions about this policy please contact Dr. Robert Hanzlik, Director, COBRE-PSF (rhanzlik@ku.edu).
3. Research support and billing information.
COBRE Core Labs operate on a fee-for-service basis. Core Lab rates are the same for all clients. The current
fees are published at http://psf.cobre.ku.edu. Fee subsidies (coupons) may be available in certain cases. In
other cases, F&A surcharges may apply. Acknowledgement and authorship are not substitutes for payment of
the costs of the research/service that the cores provide.
The PSL guarantees to perform the work requested (i.e., to execute the client-provided or client-approved
protocol) competently and faithfully, and to document steps accurately, but gives no guarantee of specific
outcomes on research-like projects (defined as not a repeat of a known successful protocol). The Core Lab
Director will notify the Client when the project will start, and will furnish brief email updates at significant milestone
events, plus a complete report at completion of the project.
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