SOP Number: CP094.00
Title: Labeling Organ Weigh Boats and Vials
Revision No: Replaces:
00
N/A
Author: Kristin Smith
I
Date in effect: Page:
7/14/2010
Page 1 of 4
MAL Director: Dr. Peggy Borum
FSHN Chair: Dr. Neil Shay
PURPOSE
The purpose of labeling the organ weigh boats and vials is to distinguish what is being collected
during non-survival surgery. Also labeling the weigh boats eliminates the potential for
contamination among multiples organs.
II
SCOPE
This procedure provides instructions for labeling weigh boats used to weight piglet organs during
non-survival surgery. This procedure also provides instructions for labeling organ vials which
contain the organs after they are processed.
III
RESPONSIBILITIES
It is the responsibility of Metabolic Assessment Laboratory personnel to follow this procedure.
It is the responsibility of supervisory personnel to ensure compliance with this procedure and to
train employees and students responsible for performing this procedure. Students will report
accidents to the principal investigators immediately.
IV
REFERENCES
N/A
V
REAGENTS AND MATERIALS
N/A
VI
EQUIPMENT
VI.A. Large & small weigh boats.
VI.B. Fine felt black marker.
VI.C. Scintillation tubes.
VI.D. Clear tape.
VII
SAFETY PRECAUTIONS
VII.A. Members of the MAL have been trained extensively in the procedures described in this
SOP.
VIII
DEFINITIONS
VIII.A. Standard Operating Procedure (SOP) – Standard Operating Procedure is a document that
provides instructions for completing a specific task in the lab.
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SOP Number: CP094.00
Title: Labeling Organ Weigh Boats
Revision No:
00
Replaces:
N/A
Date in effect:
7/14/2010
Page:
Page 2 of 4
VIII.B. Metabolic Assessment Laboratory (MAL) – The Metabolic Assessment Laboratory is
the laboratory that will use this SOP.
VIII.C. Piglet Identification (ID)—The Piglet ID is a unique, alphabetical and numerical label
that identifies the piglet and the samples taken from that piglet.
IX
PROCEDURE
IX.A. Check the list in X.B. for the necessary size of the weigh boats for each organ as well as
the appropriate number of tubes necessary to hold each organ.
IX.B. Labeling weigh boats
IX.B.1. Whenever possible, older boats can be reused. The older ones should be
ethanoled to remove any writing and they should not have many rips in the
plastic.
IX.B.2. Newer weigh boats are located in the white drawers on lab side.
IX.B.3. Label every organ’s weight boat by writing the organ name on the top lid and the
piglet ID number on the bottom lid. This should be done for every piglet.
IX.C. Labeling organ vials
IX.C.1. Obtain the necessary amount of scintillation tubes based on the amount needed in
X.B.
IX.C.2. Create computer-generated labels with the following information going from top
to bottom:
IX.C.2.a. Piglet ID
IX.C.2.b. Organ name
IX.C.2.c. Date of non-survival
IX.C.2.d. Sample number
IX.C.3. Label the caps of the tubes with the same information using a fine tip marker.
IX.C.4. Organize the labeled organ tubes in the cardboard rack according to the design in
X.C.
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SOP Number: CP094.00
Title: Labeling Organ Weigh Boats
Revision No:
00
X
Replaces:
N/A
Date in effect:
7/14/2010
Page:
Page 3 of 4
ATTACHMENTS
X.A. For a list of materials and their locations, refer to SOP# CG098.00.
X.B. Size and number of organ vials.
Vials made by: _________________________
Weigh Boats made by: _________________________
Date:_______
Date:_______
Total Number of Vacutainers: 16
Total Number of plastic vials: 49 + 4 or more extras
Total Number of Weigh Boats: 26 + 5 extras, Large Weigh Boats: 8 + 3 extras, Small Weigh Boats: 18
+ 2 extras
Organ
Adrenals
Bile
Heart
Kidneys
Liver
Lung
Muscle
Spleen
Stomach
Stomach contents
Top 1/3rd of SI
Top 1/3rd of SI contents
Middle 1/3rd of SI
Middle 1/3rd of SI contents
Bottom 1/3rd of SI
Bottom 1/3rd of SI contents
Number of Vials (49)
1
1
3
2
4
3
3
1
2
3
2
1
2
1
2
1
Size of Weigh boat
Small
Small
Small
Large
Large
Large
Large
Small
Small
x
Large
x
Large
x
Large
x
Large Intestine
Large Intestine Contents
Left Cerebrum
Left Cerebellum
Right Caudate Nucleus
2
2
1
1
1
Large
x
Small
Small
Small
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SOP Number: CP094.00
Title: Labeling Organ Weigh Boats
Revision No:
00
Replaces:
N/A
Date in effect:
7/14/2010
Right Cerebellum
Right Frontal Lobe
Right Hippocampus
Right Hypothalamus
Right Lateral Temporal Lobe
Right Medulla
Right Occipital Lobe
Right Parietal Lobe
Right Pons
Right Thalamus
Page:
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1
1
1
1
1
1
1
1
1
1
Small
Small
Small
Small
Small
Small
Small
Small
Small
Small
X.C. Layout for the cardboard racks used to the hold the organ vials.
Urine
Urine
Adrenals
Bile
Heart
Heart
Heart
Kidneys
Kidneys
Liver
Liver
Liver
Liver
Lung
Lung
Lung
Muscle
Muscle
Muscle
Spleen
Stomach
Stomach
Sto. Con.
Sto. Con.
Sto. Con.
TI
TI
TI Con.
MI
MI
MI Con.
BI
BI
BI Con.
LI
LI
LI Con.
L.
Cerebrum
L.
Cerebellum
R. Front.
Lobe
R.
Hippocam.
R.
Hypothal.
R. Medulla
R.
Occipital
R. Parietal
R. Pons
R.
R. Caudate Cerebellum
R.
Thalamus
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