Proteomics
Facility
http://www.le.ac.uk/mrctox/pnacl
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Name:________________________________________
Submission Date: _____________________
Department:__________________________________
Tel. Extn.: ___________________________
Grant Number:
E-Mail: _____________________________
______________________________
Name and E-Mail of Group Leader: ________________________________________________________________
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Number of Samples: _____________________
Please append a full list of sample names in the table on the reverse of this form
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Sample Type:  Gel Band
 Solution
Digestion Requirements:  Trypsin
 Beads
 Other
 Other enzyme/s
(default option).
(may be required to be supplied by the user. Please enquire).
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Analytical Requirements:
A.  Identification of a single protein. (e.g. 1D-band / 2D-spot / protein in solution)
B.  Identification of Components in a simple mixture of proteins. (2-20 proteins)
C.  Identification of Components in a complex mixture of protein. (>20proteins)
Please indicate estimate of number of proteins that the sample contains. Complex samples may require fractionation,
resulting in multiple LC-MS/MS runs; please discuss this with PNACL
D.  Identification of Post-translational modifications.
Where the position of a PTM is known/suspected these can be scanned for directly. Enrichment strategies can be used
for certain modifications (e.g. Phosphorylation). Please discuss PTM analysis with PNACL.
E.  Relative Quantification of protein abundances between samples.
A range of methods are available (SILAC, iTRAQ, TMT, SRM etc.) please discuss analysis with PNACL.
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Further Information:
If your protein is not a native sequence and contains tags (such as GST / His / GFP etc), point mutations or any other changes to
the sequence then you MUST supply an electronic copy of the sequence (sya2@le.ac.uk).
Organism of Interest e.g. Mouse/P.falciparum etc. _
If the organism of interest is not a model or widely studied organism the user should either provide a protein .fasta database or
indicate a publicly available database for searching
Known/suspected modifications
_______
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Sample Names
Analysis
Type
(A-E)
#
Proteins
Any Other Information
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Continue sample numbering as required