Heredity Cheat Sheet Alleles: Alternative forms of a gene Autosomal chromosome: A nonsex chromosome Chromosome: A linear or circular strand composed of DNA that contains genes Diploid: An organism with two copies of each chromosome DNA: Deoxyribonucleic acid; the molecule that carries genetic information Dominant: A phenotype or allele that completely masks the presence of the other, recessive allele in the heterozygote Gene: The fundamental unit of heredity; a specific section of DNA within a chromosome Genotype: The genetic makeup of an individual; the allele(s) possessed at a given locus Heterozygote: An individual with two different alleles of a given gene or locus Homozygote: An individual with two identical alleles of a given gene or locus Locus: A specific location on a chromosome Phenotype: The physical characteristics of an individual Recessive: A phenotype or allele exhibited only when homozygous The Structure of DNA DNA is made up of long chains of nucleotides. To make a complete DNA molecule, single nucleotides join to make chains that come together as matched pairs and form long double strands. Each nucleotide is comprised of the following: A five-sided (pentose) sugar called deoxyribose A phosphate One of four nitrogen-rich bases: adenine, guanine,cytosine, or thymine Nucleotides are joined together by phosphodiester bonds. Nucleotide chains are antiparallel and complementary. Unifying theory: DNA->mRNA->amino acids->proteins Replication (copying of DNA) Transcription (message is copied into mRNA) Translation (message in mRNA is converted to an amino acid sequence at the ribosomes [rRNA] using tRNA) Mendel's Laws of Inheritance Genetic inheritance boils down to three simple concepts put forth by Gregor Mendel, a humble monk and part-time scientist who founded the entire discipline of genetics: Segregation: In diploid organisms, chromosome pairs (and their alleles) are separated into individual gametes (eggs or sperm) to transmit genetic information to offspring. A dominant allele completely masks the effects of a recessive allele. A dominant allele produces the same phenotype in heterozygotes and in homozygotes. Independent assortment: Alleles on different chromosomes are distributed randomly to individual gametes. Genetics Cheat Sheet A = dominant a = recessive Genotypes: Actual letters representing alleles on genes AA = homozygous dominant Aa = heterozygous aa = homozygous recessive Phenotypes: outer appearance AA or Aa = dominant trait is expressed aa = recessive trait is expressed Pedigree: family tree Square: boy Circle: girl ── : mating │ : offspring Colored: have trait Punnett Square: show all possible outcomes of a mating Sex-Linked: examples: hemophilia, colorblindness h Male with trait: X Y H Male without trait: X Y h h Female with trait: X X H h Female carrier: X X H H Female without trait: X X Incomplete Dominance: the heterozygous form is a mixture of the dominant and recessive trait AA: dominant trait (red) Aa: mixture (pink) aa: recessive trait (white) Co-Dominance: the heterozygous is a combination of both dominant traits; no recessive trait st RR: 1 dominant trait (red) RR’ : Both dominant trait (roan-both red/white) nd R’R’ : 2 dominant trait (white) Blood Types: an example of codominance with a recessive A B A A A I I : AB blood I I or I i : A blood (AO or AA) B B B ii: O blood (OO I I or I i : B blood (BO or BB) Typical Crosses Homozygous dominant (AA) x homozygous recessive (aa): 100% of offspring are heterozygous (Aa) Heterozygous dominant (Aa) x Heterozygous dominant (Aa) 25% AA/50% Aa/25% aa or a 1:2:1 ratio Heterozygous dominant (Aa)x homozygous recessive (aa): 50% Aa/ 50% aa (half show recessive trait) or 1:1 Dihybrid crosses: AaBb x AaBb 9:3:3:1 (9/16 will have show both dominant traits, 1/16 will have both recessive traits) Mitosis/Meiosis “Cheat” Sheet Biological Reproduction and Cell Division Biology is all about life. And life is really all about continuation, and in order for life to continue, reproduction has to occur at the cellular level so that genetic information (DNA) is replicated. The following concepts (mitosis and meiosis) are key to understanding how cells reproduce: Cells do mitosis when they are going to make an exact copy of themselves for asexual reproduction, growth, or tissue repair. DNA replication occurs once, followed by a single division. The parent and daughter cells are both diploid, which means they have a double set of chromosomes.Cells do meiosis in order to produce gametes (eggs and sperm) for sexual reproduction. DNA replication occurs once, followed by two divisions. The parent cell is diploid, but the daughter cells are haploid, which means they have half the number of chromosomes as their parent cells. Mitosis: Mitosis is used to duplicate cells or for the repair of damaged cells and growth of the organism. Mitosis occurs most in the cells in organs (such as the skin, stomach) that get the most wear. It produces 2 genetically identical cells that have 2 full sets of chromosomes and are therefore diploid. One set of chromosomes came from each parent – this is the number of chromosomes in all somatic cells (body cells). The cell goes through 1 cycle of the 5 phases of mitosis Prophase – where DNA condenses for form visible chromosomes. Metaphase – where the chromosomes line up in the center of the cell Anaphase- where the chromosomes start to separate Telophase – where the two cells begin to separate Cytokinesis - where the two cells complete their separation and begin to grow During S phase of the cell cycle (but not a part of mitosis) the DNA is replicated During interphase (also, not part of mitosis) the cell is performing its function for the organs – Cells spend most of their time in interphase Meiosis For production of gametes, the sex cells, sperm and egg, meiosis produces 4 cells that are genetically different. Each gamete has only 1 set of chromosomes (1N). The gametes are haploid. During fertilization, the egg and sperm join and create a zygote that has the full 2 sets of chromosomes. In meiosis, the cell goes through 2 cycles of the 5 phases of mitosis. In Prophase I small pieces of DNA may cross over from one chromosome to the other – this is called crossingover. During crossing-over, there is increased genetic recombination. Throughout the first cycle (Prophase I – Cytokinesis I) the duplicated chromosomes remain connected at the centromere. At this point, the cells are haploid or 1N. Prohpase II through Cytokinesis II are very similar to these phases of mitosis, except there is no duplication of the DNA –the duplicated chromosomes are separated and the cell divides.