OBSERVED CONFLICT AND ASTHMA Supplementary Online Material (SOM) for Naturalistically-Observed Conflict and Youth Asthma Symptoms [MASKED AUTHOR INFORMATION] This file includes: Supplementary Methods Supplementary Results Table S1 Table S2 Table S3 Supplementary References 1 Supplementary Methods Multiple Imputation Multiple imputation was applied to account for the missing data and to improve statistical power (Graham, 2009; Graham, Cumsille, & Elek‐Fisk, 2003). We used a multiple imputation approach under a model that assumes values are missing at random meaning that the missing values do not depend on unobserved data, given the availability of observed outcome data and covariates (Little & Rubin, 1989). Thirty imputations were completed as recommended by Graham et al. (2007) to prevent a falloff of statistical power. All analyses were carried out using SPSS version 22 software. Supplementary Results Naturalistically-Observed and Self-Reported Conflict We first examined bivariate correlations between caregiver-reported, youth-reported, and EAR-observed measures of conflict. Table S1 presents bivariate correlations between all study measures using imputed data. Notably, there were no associations between EAR-observed conflict composites and the baseline self-reported measures of conflict (e.g., caregiver and youth PEQ conflict). However, daily diary reports of negative interactions from the caregiver were significantly associated with baseline caregiver-reported conflict (e.g., caregiver-PEQ), youth daily reports of negative interactions, EAR-observed measures of interpersonal conflict, and EAR-observed caregiver-youth conflict. Additionally, youth daily diary reports of negative interactions were significantly correlated with baseline youth-reported conflict (e.g., youthPEQ). Further, EAR-observed interpersonal conflict and EAR-observed caregiver-youth conflict were strongly associated, as would be expected given the composite codes share overlap (e.g., yelling by the mother and yelling by the youth). Conflict and Asthma Symptoms We next explored the associations between the measures of conflict and asthma symptoms (See Table S2). Notably, there were no significant relationships between caregiverPEQ, youth-PEQ, and self- or daily reported asthma symptoms or EAR-observed wheezing. However, with the daily diary reports of negative interactions, a relationship emerged. Daily youth reports of negative caregiver-youth interactions were significantly associated with greater daily asthma symptoms and marginally related with greater self-reported asthma symptoms. EAR-observed interpersonal conflict was significantly associated with greater baseline selfreported asthma symptoms and daily asthma symptoms and marginally related with greater EAR-observed wheezing. EAR-observed caregiver-youth conflict was significantly associated with greater EAR-observed wheezing and marginally related to baseline self-reported asthma symptoms. Interpersonal Conflict and Asthma Symptoms We next conducted several hierarchical multiple regression analyses to examine the independent effects of EAR-observed interpersonal conflict and daily diary measures of conflict on asthma symptoms. As displayed in Table S2, EAR-observed interpersonal conflict and the daily diary indices of conflict (e.g., youth-reported negative caregiver-youth interactions and caregiver-reported negative interactions) were entered as predictors. EAR-observed interpersonal conflict significantly predicted increased baseline self-reported asthma symptoms and, when daily reports of conflict where added to the regression, significantly predicted increased self-reported asthma symptoms. EAR-observed interpersonal conflict also significantly predicted increased asthma symptoms reported via daily diary and when daily reports of conflict where added to the regression equation, the relationship was marginally significant. Notably, when entering daily reports of conflict, youth daily ratings of caregiver-youth conflict significantly predicted daily reported asthma symptoms. EAR-observed interpersonal conflict marginally predicted increased EAR-observed wheezing. When including daily reports of caregiver-youth conflict in the regression, EARobserved interpersonal conflict and youth daily ratings of caregiver-youth conflict were both marginally significant. After the addition of youth ethnicity, the relationship between EARinterpersonal conflict and EAR-observed wheezing remained marginally significant. Caregiver-Youth Conflict and Asthma Symptoms We then conducted several hierarchical multiple regression analyses to examine the independent effects of EAR-observed caregiver-youth conflict and daily diary measures of conflict on asthma symptoms. As displayed in Table S3, EAR-observed caregiver-youth conflict and the daily diary indices of conflict (e.g., youth-reported negative caregiver-youth interactions and caregiver-reported negative interactions) were entered as predictors. EAR-observed caregiver-youth conflict was marginally associated with increased self-reported asthma symptoms. When adding daily measures of conflict into the regression equation, EAR-observed caregiver-youth conflict and youth daily ratings of caregiver-youth conflict marginally predicted increased self-reported asthma symptoms. EAR-observed caregiver-youth conflict was not associated with daily reported asthma symptoms. When including daily diary reports of conflict, youth reports of negative interactions emerged as a significant predictor of daily reported asthma symptoms and not EAR-observed caregiver-youth conflict. Finally, EAR-observed caregiver-youth conflict significantly predicted EAR-observed wheezing. When including daily reports of caregiver-youth conflict in the regression, EARobserved caregiver-youth conflict remained significant. After the addition youth ethnicity, the relationship between EAR-observed caregiver-youth conflict and EAR-observed wheezing remained significant. OBSERVED CONFLICT AND ASTHMA 6 Table S1 Bivariate Correlations between Study Variables using Imputed Data 1. Y-Age 2. Y-Ethnicity 3. Y-PEQ 4. C-PEQ 5. Y-NG 6. C-NG 7. EAR-Observed Interpersonal Conflict 8. EAR-Observed Caregiver-Youth Conflict 9. Self-Report Asthma Symptom Questionnaire 10. Daily Diary Asthma Symptom Composite 11. EAR-Observed Wheezing 2 3 .08 .06 -.10 4 .04 .06 .38** 5 .12 -.11 .44** .34** 6 .01 .01 .12 .42** .38** 7 -.07 .01 -.03 .13 .24 .43** 8 .05 .07 -.01 .09 .24 .44** .80** 9 -.02 .06 .07 -.10 .23+ .03 .28* .25+ 10 .07 -.04 .21 -.01 .40** .10 .28* 11 .01 .21 -.27 -.16 -.07 .08 .30+ .18 .34* .49** .15 -.08 Note. + p < .10. * p < .05. ** p < .01. Y = Youth-Reported. C = Caregiver-Reported. PEQ = Parental Environment Questionnaire. NG = Daily Negative Caregiver-Youth Interactions. EAR = Electronically Activated Recorder. Table S2 Multiple Regression Analyses Pooled Coefficients after Multiple Imputation with EAR Interpersonal Conflict and Daily Caregiver- and Youth-Reports Predicting Asthma Symptoms Predictor Variables Step 1 EAR-Observed Interpersonal Conflict Step 2 EAR-Observed Interpersonal Conflict Y-NG C-NG Step 3 EAR-Observed Interpersonal Conflict Y-NG C-NG Y-Ethnicity Self-Reported Asthma Symptoms Daily Diary Reported Asthma Symptoms EAR-Observed Wheezing b-weight (SE) b-weight (SE) b-weight (SE) .42 (.19)* .46 (.21)* 2.47 (1.46) + -.28 (.21) .04 (.02)* .04 (.02)+ .46 (.14) ** -.03 (.02) .30 (.15) + .35 (.18) + -1.08 (1.59) + -.02 (.19) .34 (.17) + -.89 (1.57) -.03 (.19) .26 (.16) Note. + p < .10. * p < .05. ** p < .01. Y = Youth-Reported. C = Caregiver-Reported. NG = Daily Negative Caregiver-Youth Interactions. Table S3 Multiple Regression Analyses Pooled Coefficients after Multiple Imputation with EAR Caregiver-Youth Conflict and Daily Caregiver- and Youth-Reports Predicting Asthma Symptoms Predictor Variables Step 1 EAR-Observed Caregiver-Youth Conflict Step 2 EAR-Observed Caregiver-Youth Conflict Y-NG C-NG Step 3 EAR-Observed Caregiver-Youth Conflict Y-NG C-NG Y-Ethnicity Self-Reported Asthma Symptoms Daily Diary Reported Asthma Symptoms EAR-Observed Wheezing b-weight b-weight b-weight .35 (.19) + .38 (.21) + 2.52 (1.47) + -.27 (.21) .03 (.02) .02 (.02) .48 (.14)** -.02 (.02) .31 (.13)* .36 (.15)* -1.08 (1.52) -.05 (.19) .35 (.15)* -.91 (1.50) -.05 (.19) .23 (.16) Note. + p < .10. * p < .05. ** p < .01. Y = Youth-Reported. C = Caregiver-Reported. NG = Daily Negative Caregiver-Youth Interactions. Supplementary References Graham, J. W. (2009). Missing data analysis: Making it work in the real world. Annual review of psychology, 60, 549-576. Graham, J. W., Cumsille, P. E., & Elek‐ Fisk, E. (2003). Methods for handling missing data. Handbook of psychology. Graham, J. W., Olchowski, A. E., & Gilreath, T. D. (2007). How many imputations are really needed? Some practical clarifications of multiple imputation theory. Prevention Science, 8(3), 206-213. Little, R. J., & Rubin, D. B. (1989). The analysis of social science data with missing values. Sociological Methods & Research, 18(2-3), 292-326.