Anti Fibronectin expression in lung, liver, spleen, Kidney and Thymus
during aspergillosis infection using Immunohistochemical technique.
Batol Imran Dheeb, Basim Mohammed Khashman b
a Iraqia University, b Bghdad university
a batoolomran@yahoo.com
Abstract
The proteins laminin and fibrinogen are candidates for mediating
adherence of conidia to the extracellular matrix to basement
membranes and to the fibrin and fibrinogen deposits formed in
response to the inflammatory reactions at the surfaces of wounded
epithelia conidia of Aspergillus fumigates are able to adhere to
fibrinogen laminin and complement via proteins of the outer cell wall
Fibronectin is a disulfide - linked dimeric glycoprotein present in a
soluble form in blood plasma and other body fluids and in a fibrillar
form in extracellular matrices .
The major function of fibronectin is probably related to its
ability to mediate substrate adhesion to mammalian cells, a
process that involves the binding of specific cell surface
receptors to discrete domains in the fibronectin molecule
Anti fibronectin were investigated in BALB/c mice infected
intravenously with 5×106 virulent Aspergillus fumigatus conidia by using
immunohistochemistry technique using detection kit(ab80436) and anti
fibronectin marker (ab2413) Five groups of animals were studied,
including control group (mice not infected with A. fumigatus) these
groups were used to study the expression in Two separated periods 7 and
14 day post infection. Each section of socket tissue from organs sample
was evaluated for the presence of intracellular brown DAB precipitate
indicative of antibody binding. The staining intensity was assessed using
a designed scoring system .
Expression of Anti fibronectin in the studied organs were shown in
the cytoplasm of the tissue cells and detected by IHC technique .
Depending on the scoring system used for the Anti fibronectin were the
intensity of the staining of the cytoplasim used as parameters dependent.
The intensity of the cytoplasim of the stained cells was negative if there is
no expression. Expression was found positive in all the studied organs,
but different in intensity, in lung over expression represented by strong
staining (score 3+) in 7 and
14 days post infection with moderate
staining at 7 day and intense staining at 14 day respectively, in liver
expression was (score 2+ and 3+) with moderate staining at 7 and 14 day
post infection. The expression in the spleen kidney and thymus
was
(score1+) with light staining at the period between 7 to 14 day . Anti
fibronectin expression in all studied sample compared with the positive
control, Results show significant difference (P value >0.01) in the
expression between the studied organs.
In conclusion, Anti fibronectin expression levels was timedependent increased and related with the progress of infection that can
significantly enhance resistance to A. fumigatus in BALB/c mice.
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