Transgenic and Gene Targeting Core Laboratory
ES Cell Services
Investigator:
SDRC Member?
Speed Type:
;
CCTSI Member?
Contact:
Date:
eRA Commons Name:
Email:
Phone:
Gene/Transgene/Construct Name:
Abbr.:
____________________________________________________________________________________
Submission Information:
DNA Preparation: The Investigator is responsible for purifying and linearizing 30-50ug of DNA according to the protocol
provided. Please provide DNA at 1ug/µl concentration in sterile TE buffer (10mM Tris-HCl, pH7.6, 1mM EDTA) clearly labeled
with PI’s name, construct name, concentration, and date.
DNA DROP-OFF Location: RC-1 North, 8th floor, room P18-8403E. Place samples in the Kenmore Elite refrigerator (outside
Koch Lab) labeled “Transgenic Core”. Please see receptionist, room P18-8130(admin office) if you do not have access to this
lab area. Please send an email notification to Makeamouse@ucdenver.edu before dropping off DNA.
All samples submitted will go through quality control and assurance process before initiating the experiment. For more
information and questions regarding DNA preparations or ES targeting process please contact core personnel at
makeamouse@ucdenver.edu.
Please supply the construct at a concentration of 1 µg/µl dissolved in sterile TE buffer (10mM Tris-HCL pH7.6, 1mM
EDTA).
Construct preparation date:
Total amount of DNA supplied:
Construct Size:
A260/A280 ratio:
Targeting Construct DNA derived from:
Selection Markers:
ES cell line to be targeted (Additional
information on these ES cell lines can be
found here
µg
Kb
(30µg minimum)
129Sv
C57BL/6
Neomycin/G418,
TK,
CJ7 (129Si)
BL6N (JM8A1.N3)
Gene Targeting Service
Electroporation/Drug Selection (First 288 clones)
Additional Clones (Per 96 well plate)
Clone Expansion (up to 6 Clones)
Karyotyping (cost per clone) [mandatory before blastocyst injection]
Removal of loxP- or FRT-flanked genomic sequences by transient
transfection of ES cell clones with Cre expression plasmids or FLPE
expression plasmids
(contact us at Makeamouse for details)
Total Charges
DTA
Hybrid EC7.1
Non-SDRC
SDRC
Qty
Total
$3,114
$2,345
0
$0
$879
$655
0
$0
$1,057
$822
0
$0
$1,454
$1,123
0
$0
$3,114
$2,345
0
$0
$0
Appl ESI service req.docx, revised 12-2011 sw/ja
Please include a photograph of a gel showing undigested and linearized DNA side by side
Appl ESI service req.docx, revised 12-2011 sw/ja