This Journal Belongs to: _________________ The NERVOUS SYSTEM & SENSES ANATOMY & PHYSIOLOGY JOURNAL Types of Neurons The Synapse Structure of Neurons Nerve Impulses “All Or Nothing” Law Resting Neuron Movement of the Impulse Neural Impulse Terms The Brain Structure of the Brain The Spinal Cord The Eye and Vision Functions of the Brain The Reflex Action The Ear: Hearing and Balance Chemical Senses (Nose & Tongue): Smell and Taste 1 THE NERVOUS SYSTEM The nervous system is the master coordinating system of the body. Every thought, action, and sensation reflects its activity. The structures of the nervous system are described in terms of two principle divisionsthe central nervous system (CNS) and the peripheral nervous system (PNS). The CNS (brain & spinal cord) interprets incoming sensory information and issues instructions based on past experience. The PNS (cranial and spinal nerves & ganglia) provides the communication lines between the CNS and the body’s muscles, glands, and sensory receptors. The nervous system is also divided functionally in terms of motor activities into the somatic and autonomic divisions. These divisions are important, however, one must recognize that these classifications are made for the sake of convenience and that the nervous system acts in an integrated manner both structurally and functionally. Overall, it is imperative that one understands that every body system is controlled, at least in part, by the nervous system. 1. List the three major functions of the nervous system. a. ______________________________________________________________________ ______________________________________________________________________ b. ______________________________________________________________________ ______________________________________________________________________ c. ______________________________________________________________________ ______________________________________________________________________ 2. Choose the key responses that best correspond to the descriptions provided in the following statements. Insert the appropriate letter in the blank. Key Choices: 3. A. Autonomic Nervous System C. Peripheral Nervous System ______ 1. NS subdivision that is composed of the brain and spinal cord. ______ 2. Subdivision of the PNS that controls voluntary activities such as activation of the skeletal muscles. ______ 3. NS subdivision that is composed of cranial nerves and spinal nerves and ganglia. ______ 4. Subdivision of the PNS that regulates the activity of the heart and smooth muscle, and of glands; it is also called the involuntary nervous system ______ 5. A major subdivision of the NS that interprets incoming information and issues orders. ______ 6. A major subdivision of the NS that serves as communication lines, linking all parts of the body to the CNS. Nervous Tissue is made up of neurons and neuroglia both specialized cells. Indicate which type of cell is identified by the following descriptions. Insert the appropriate letter in the answer blank. Key Choices: 4. B. Central Nervous System D. Somatic Nervous System A. Neurons B. Neuroglia ______ 1. Support, insulate, and protect cells. ______ 2. Demonstrate irritability and conductivity, and thus transmit electrical messages from one area of the body to another area. ______ 3. Release neurotransmitters. ______ 4. Are able to divide; therefore responsible for most brain neoplasms (an abnormal new growth of tissue) Relative to neuron anatomy, match the anatomical terms given in Column B with the appropriate descriptions of functions provided in Column A. Place the correct term on the line. Column A Column B ______ 1. Releases neurotransmitters A. Axon ______2. Conducts electrical currents towards cell body B. Axon terminal ______3. Increases the speed of impulse transmission C. Dendrite ______4. Location of the nucleus D. Myelin sheath ______5. Generally conducts impulses away from the cell body E. Cell Body 2 5. Label the motor neuron and trace the impulse. 6. Classify the neurons below: _____________________ 7. __________________ ____________________ Read the passage and fill-in the blanks. Hint: All answers can be found in the reading Human beings respond to their environment in pretty similar way as other animals. Human _______________controls these responses with the outer world. Human nervous system is a complex system comprising of millions of neurons. The function of these neurons can be classified into two major types, firstly bringing the stimuli from the peripheral organ to the central nervous system and second carrying the responses from the central nervous system to the peripheral organs. Afferent and efferent neurons are the two types of nerve fibers which perform these functions. Let us understand in detail the comparison between afferent vs. efferent neurons. Difference Between Afferent and Efferent Neurons Before we proceed to the comparison between afferent vs. efferent neurons it is imperative that we gain some insight upon the processing of impulses by the nervous system. Nervous system comprises of a closed loop of neurons which deal which sensation, decision and reaction. Whenever an impulse or stimuli is received by a receptor organ, it is carried to the brain for processing. A decision is made regarding the impulse which is again carried back to the receptor organ. Depending upon this decision a reaction to the impulse is produced by the receptor organ. Three types of neurons take part in this entire cycle, namely afferent, interneurons and efferent neurons. Afferent neurons are concerned with carrying the impulse from the receptor organ towards the ________________ whereas efferent neurons carry the response of the brain back to the _________________. Both these neurons communicate with each other through the medium of ______________________________. Afferent neurons are also called as _________________________ neurons as they mostly carry impulses from sensory organs. Afferent neurons are classified as pseudounipolar neurons with a single long dendrite and a short axon. The axon extends in both directions, with peripheral axon directing towards the receptor organ, whereas central axon passing into the spinal cord. Although, dendrites are structurally and functionally identically to axons, they are myelinated. The cell body in afferent neurons is perfectly rounded and smooth. The aggregation of afferent neurons can be found in a swelling called dorsal root ganglion, which is located just outside the spinal cord. Efferent neurons are also called as _______________________________as they mostly carry responses to the muscles or glands and bring about movement. Efferent neurons are bipolar with dendrite on one end and axon on the other. The cell body is connected at one end to a single long axon while several dendrites form the other end of the cell body. The cell body in efferent neurons is satellite shaped. The impulse enters the cell body via several dendrites and then leaves it through the single axon at the other end. The efferent neurons are present in the gray matter of spinal cord as well as medulla oblongata. The efferent neuron forms an electrochemical pathway towards the effector organ. Afferent neurons are connected to efferent neurons via multipolar neurons called ___________________. Interneurons are also called as relay neuron, association neuron or local circuit neuron. Similar to efferent neurons, the cell bodies of interneurons are located inside the central nervous system. Interneurons vary greatly in structure and function. Hence, it is impossible to predict the types of interneurons present in the central nervous system. It is estimated that human brain contains about 100 billion interneurons with an average of 1000 synapses, on each interneuron. The most important point of comparison between afferent vs efferent neurons is that they perform an exactly opposite function and follow an opposite electrochemical pathway in the central nervous system loop. Flow Chart that Depicts Afferent Vs. Efferent Responses 3 Introduction to the Nervous System Lab: Reaction Time of An Impulse PROBLEM: What can change reaction time? Introduction: The human nervous system is composed of the brain and spinal cord (Central Nervous System, CNS) and the nerves which branch out from the CNS, the Peripheral Nervous System (PNS). Sensory neurons of the PNS carry information to the CNS. Signals from the brain are carried to motor neurons (PNS), which carry out responses by muscles. In this lab, you will be comparing the rate at which sensory neurons, working through the brain, can elicit responses via motor neurons. Basically, you will test reaction time. Reaction time is how long it takes for a message to travel along your nerve pathways. Materials: Metric ruler Calculator Procedure: 1) Have a partner hold a metric ruler at the end with the highest number. Note: Both partners should do the test. 2) Place the thumb and first finger of your left hand close to, but not touching, the end with the lowest number. 3) When your partner drops the ruler, try to catch it between your thumb and finger. 5) Record where the top of your thumb is when you catch the ruler. Make your measurements to the nearest 0.5cm. Put this number in your data table as trial 1. At any time if you do not catch the ruler in time, record this as 35 cm. 6) Repeat steps 2 to 5 three more times. 7) State if you think the ruler will fall farther if you catch it with your right hand. 8) Repeat steps 2 to 5 four times using your right hand to catch the ruler. 9) Switch roles and drop the ruler for your partner. 10) Calculate: To complete your data table, calculate the time in seconds needed for the ruler to fall. Do the following for each trial: Steps for Calculation: 1) Multiply the distance in cm by 2. 2) Divide the result in step 1 by 1000. 3) Calculate the square root of the result in step 2. Round all answers to 2 decimal places. 11) Find the average for each column. 12) Answer all discussion questions Trial Left Hand Distance Ruler Falls Time in Seconds (cm) Right Hand Distance Ruler Falls Time in Seconds (cm) 1 2 3 4 Total Average 4 Discussion Questions: 1) Which hand is your writing hand? _______________________________________________________________________ 2) Did you catch the ruler faster with your left hand or right hand? Why might this be so? __________________________________________________________________________ __________________________________________________________________________ 3) Why did you run several trials for each hand? __________________________________________________________________________ __________________________________________________________________________ 4) Explain why a message moving along nerve pathways takes time. __________________________________________________________________________ __________________________________________________________________________ 5) How might the results change if you did this experiment with a person of 70 years old? Why might this be so? __________________________________________________________________________ __________________________________________________________________________ 6) How might the results change if you did this experiment with a professional athlete? Why might this be so? __________________________________________________________________________ __________________________________________________________________________ 7) What is the definition of reaction time? __________________________________________________________________________ __________________________________________________________________________ 5 “Lights, Camera, Action Potential” Directions: Go to the following website http://faculty.washington.edu/chudler/ap.html.Read “Lights, Camera, Action Potential and then answer the questions below. 1. Describe the characteristics of a resting membrane. 2 points _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ 2. Define the terms threshold potential, action potential, nerve impulse, and re-polarization. 3 points _______________________________________________________________________ _______________________________________________________________________ _______________________________________________________________________ 3. Analyze the illustration below. For each step, describe the events involved in the propagation of an action potential in a neuron. 5 points A.______________________________ ________________________________ ________________________________ B.______________________________ ________________________________ ________________________________ C.______________________________ ________________________________ ________________________________ D.______________________________ ________________________________ ________________________________ E.______________________________ ________________________________ ________________________________ 4. Define the terms synapse and neurotransmitter (NT), and discuss the steps involved in the synaptic transmission of a nerve impulse from one neuron to another. 5 points 6 Steps: 5. Color and label the picture below. Make sure you identify the neurotransmitter, pre-synaptic terminal, post-synaptic terminal The Synapse 6. Circle the term that does not belong in each of the following groupings. a. Neurotransmitter Synapse Axon b. K+ enters cell K+ leaves cell Repolarization c. Nodes of Raniver Myelin sheath Unmyelinated d. Inside cell High Na+ Low Na+ 7 Message Transmission Messages can travel in neurons at speeds up to 268 miles/hr! These signals are transmitted from neuron (nerve cell) to neuron across "synapses." Let's make a chain of neurons...have everyone stand up and form a line. Each person in the line is a neuron. As shown in the figure on the right, your left hands are the dendrites of a neuron; your body is the cell body; your right arm is an axon and your right hand is the synaptic terminal. Your right hand should have a small vial of liquid or some other item, such as a button or pebble, to represent neurotransmitters. Each person should be about arms length away from the next person. When the leader says "GO," have the person at the beginning of the line start the signal transmission by placing his or her "neurotransmitter" into the hand of the adjacent person. Once this message is received, this second neuron places its neurotransmitter into the dendrite of the next neuron. The third neuron then places its neurotransmitter into the dendrites of the next neuron and the "signal" travels to the end of the line. The transmission is complete when the "signal" goes all the way to the end of the line. Remember that each "neuron" will pass its own transmitter to the next neuron in line. Each neuron HAS ITS OWN neurotransmitter. Let's review What are the parts of a neuron? The hand that receives the neurotransmitter is the "dendrite." The middle part of your body is the "soma" or "cell body." The arm that passes the neurotransmitter to the next person is the "axon" and the hand that gives the slap is the "synaptic terminal". In between the hands of two people is the "synaptic gap". For more about the parts of a neuron, see cells of the nervous system and the synapse. Measure how long it takes the message to get from the first neuron to the last. Also, measure the distance from the first to the last neuron. Now calculate the speed (speed = distance/time). How fast did the message travel from first to last neuron? Why do you think the speed of transmission of the model is so slow? Record your answers on the line below. __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ 8 Action Potential Game Objective: Race to raise the resting potential above threshold to fire an action potential. Background: When neurotransmitters cross a synapse, they can bind with receptors on dendrites. This binding can result in a change in the electrical potential of a neuron. An excitatory postsynaptic potential occurs with the neuron becomes depolarized, raising the electrical potential from its baseline of about -70 mV and bringing it closer to threshold and increasing the chance that an action potential will fire. An inhibitory postsynaptic potential occurs when the electrical potential is lowered, making it less likely an action potential will be generated. If the electrical potential is raised so that it reaches the threshold, an action potential will fire down the axon of a neuron. How to Play: Players should be divided into two teams: the Excitatory Postsynaptic Potential (EPSP) Team and the Inhibitory Postsynaptic Potential (IPSP) Team. The teams will race to see who can get the greatest signal to their team's cell body in 30 seconds. Each team lines up to act like a dendrite. A signal, (a small ball), is passed from person to person much like how an electrical signal travels down a dendrite toward the cell body. Each EPSP team signal successfully transferred to the cell body is worth +5 or +10 mV (millivolts); each IPSP Team signal is worth -5 or -10 mV. The signals are passed down the dendrites until they reach the end and are tossed into the cell body container. Only one signal ball can be passed at a time meaning that a dendrite must drop the ball (signal) into the cell body container before the first person in the dendrite can pass the next ball (signal). To Win: The typical resting potential of a neuron is -70 mV. To cause an action potential the membrane potential must reach -55 mV. Therefore at the end of 30 seconds the signals are summed from the cell body container. The total amount of millivolts is added to -70 mV to see if an action potential is fired. If an action potential is fired the EPSP team wins! If not then the IPSP team wins! Materials: 3 large containers or Tupperware About 32 ping pong balls, labeled with black marker -5, +5, -10, +10 (8 of each). Each ball should also be labeled with the team name: EPSP or IPSP. Game Set-up 9 Reflexes Reflexes are interesting and can be life-saving responses. The following activities will help you understand reflex action. Partners should switch roles during each activity so each person gets to try it. 1. Patella Reflex Sit on a chair with one leg crosses over the other. Have your lab partner gently tap your leg with a reflex hammer, just below the kneecap. If struck in the correct place, your leg should jerk forward. Repeat the procedure, but this time try to keep your leg from jerking. Can you stop this reflex? ________ 2. Achilles’ Reflex Kneel on one knee on a chair while standing with your other foot on the floor. Have your lab partner tap the Achilles tendon just above your heel with a reflex hammer. What happens? ___________________________________________________________________________________ 3. Pupil Reflex The iris is the colored part of the eye. It controls the amount of light entering the eye through the pupil. Have your partner close his eyes and also cover them with his hands for thirty seconds. Observe what happens to the pupils when he opens his eyes. Do the pupils get smaller or larger? _______________ Now, have your partner close and cover his eyes again. This time, however ask him to concentrate very hard on keeping his pupils from changing size when you signal him to open his eyes. Can he control the size of his pupils? _______________ How is it that the eye’s response to light is considered a reflex action? ________________________________________________________________________________ 4. Eye Blink Reflex Sit with your elbows resting on the lab table and your chin in your cupped hands. Have your partner hold a drinking straw about two inches from one of your eyes and blow gently through the straw. Be careful not to blow too hard or poke the eye. Did the air make you blink your eye? _________________ 5. Reflex Pathway Reflex pathways are very simple responses to stimuli. Although you are consciously aware of some of them, they are not normally under conscious control. Spinal reflexes involve a sensory nerve which receives the stimulus, a motor nerve which controls the muscular response, and the spinal cord, in which the impulse is transferred from the sensory nerve to the motor. In the box below, illustrate the reflex pathway when a finger is pricked by a pin. The pin is the stimulus. Use arrows to show the direction of the impulse in the reflex pathway. Finally, label the sensory nerve, spinal cord, motor nerve, and muscle. 10 Brain Dissection Sheep brains, although much smaller than human brains, have similar features and can be a valuable addition to anatomy studies. See for yourself what the cerebrum, cerebellum, spinal cord, gray and white matter and other parts of the brain look like! Use this as a dissection guide complete enough for a high school lab, or just look at the labeled images to get an idea of what the brain looks like. Observation: External Anatomy A. You'll need a preserved sheep brain for the dissection. Set the brain down so the flatter side, with the white spinal cord at one end, rests on the dissection pan. Notice that the brain has two halves, or hemispheres. Can you tell the difference between the cerebrum and the cerebellum? _________Do the ridges (called gyri) and grooves (sulci) in the tissue look different? ___________ How does the surface feel? _______________________________________________________________________________ B. Turn the brain over. You'll probably be able to identify the medulla, pons, midbrain, optic chiasm, and olfactory bulbs. Find the olfactory bulb on each hemisphere. These will be slightly smoother and a different shade than the tissue around them. The olfactory bulbs control the sense of smell. The nerves to the nose are no longer connected, but you can see nubbly ends where they were. The nerves to your mouth and lower body are attached to the medulla; the nerves to your eyes are connected to the optic chiasm. Using a magnifying glass, see if you can find some of the nerve stubs? _________ Observation: Internal A. Use the labeled picture to identify the corpus callosum, medulla, pons, midbrain, and pituitary gland. Use your fingers or a teasing needle to gently probe the parts and see how they are connected to each other. What does that opening inside the corpus callosum lead to? ____________________ How many different kinds of tissue can you see and feel? _________________________________________________________________________________________ 11 B. Look closely at the inside of the cerebellum. You should see a branching "tree" of lighter tissue surrounded by darker tissue. The branches are white matter, which is made up of nerve axons. The darker tissue is gray matter, which is a collection of nerve cell bodies. You can see gray and white matter in the cerebrum, too, if you cut into a portion of it. C. You can also use the letter labels on the internal anatomy picture to try to find the following: Ventricles contain cerebrospinal fluid The occipital lobe receives and interprets visual sensory messages The temporal lobe is involved in hearing and smell. You can find this by looking on the outside of one of the hemispheres. You will see a horizontal groove called the lateral fissure. The temporal lobe is the section of the cerebrum below this line. The frontal lobe also plays a part in smell, plus dealing with motor function The parietal lobe handles all the sensory info except for vision, hearing, and smell. The thalamus is a "relay station" for sensory information. It receives messages from the nerve axons and then transmits them to the appropriate parts of the brain. The pineal gland produces important hormones. D. It has been said that the human brain is the most complex 3lb bit of organized matter known in the universe. It is a fact that while you are reading this sentence, your brain is also maintaining all the systems of your body, including breathing, heart rate, body temperature, and thousands of other body functions. At this moment you are using the most marvelous living computer ever conceived. Below are pictures of various animal brains including man’s. Using a color pencil, color in the cerebrum (marked) on each brain below. a. b. Does the relative size of the cerebrum compared to the rest of the brain, seem to be related to the organism’s “intelligence” or level of nervous response? Explain. __________________________________________________________________________________ __________________________________________________________________________________ What relationship do you see, if any, between relative cerebrum size and learning ability? (For example, birds can be trained to talk, while fish cannot generally be taught to do much more than come to a food source.) __________________________________________________________________________________ __________________________________________________________________________________ E. Make a color key for the external human brain drawing on the left and then color the picture accordingly. Label the following parts on the cross section of the human brain on the right: Cerebrum, Corpus Callosum, Thalamus, Hypothalamus, and Hippocampus. Cerebrum Cerebellum Medulla Spinal Cord 12 Which Side is Dominant? Many things that you do with the right side of your body are controlled by your brain’s left side and vice versa. The following activities will help you determine which side of your brain is dominant. Record your data to each step in the table below. 1. Which hand, left or right, you use to write your name and to wave “hello.” 2. Draw a simple outline drawing of a side view of a horse in the box and mark in the table below what direction the horse faces. 3. Which way you swing a baseball bat. If you swing the bat left-handed (to the right), check the left column. If you swing the bat right-handed (to the left), check the right column. 4. Which foot, left or right, you start down stairs, and which foot you begin skipping on. 5. Which thumb is on top when you fold your hands. 6. Look at the clock while holding your finger about 6 inches in front of your face. Line your finger up with the clock while keeping both eyes open. Close your left eye. Open it and then close your right eye. Which eye is lined up with your finger and the clock. 7. Which leg your weight is on when you are at rest. 8. Draw a circle with your right hand in the space below. Note which direction you drew it. Draw a circle with left hand in the other space. Which way did you draw it? If you drew both circles clockwise, mark the right column. If you drew both circles counterclockwise, mark the left column. If you drew once circle in each direction, check both columns. Data Table: Questions: Procedure Step 1 2 3 4 5 6 7 8 Test Left Right Writing Waving Drawing a horse Starting down stairs Skipping Folding hands Looking a distance object Drawing Circles Which column has more marks? _______ Which side of your brain is dominant? _______ Which side of the body seems to be dominant? _______ 13 Mr. Egghead - The Cerebrospinal Fluid The cerebrospinal fluid (CSF) has several functions. One of these functions is to protect the brain from sudden impacts. To demonstrate how this works, we need to bring in "Mr. Egghead." Mr. Egghead is a raw egg withdrawn-on face. The inside of the egg represents the brain and the egg shell represents the pia mater (the inner most layer of the meninges or coverings of the brain). Put Mr. Egghead in a container (Tupperware works fine) that is a bit larger than the egg. The container represents the skull. Now put a tight top on the container and shake it. You should observe that shaking the "brain" (the egg) in this situation results in "damage" (a broken egg). Now repeat this experiment with a new Mr. Egghead, except this time, fill the container with water. The water represents the cerebrospinal fluid. Note that shaking the container does not cause the "brain damage" as before because the fluid has cushioned the brain from injury. You could make this into a science fair project: test the hypothesis that "The cerebrospinal fluid and skull protect the brain from impact injury." Drop Mr. Egghead from a standard height (or heights) in different conditions: 1) with fluid in the container, 2) without fluid in the container, 3) with different fluids or materials (sand, rocks) or 4) in different shaped containers, etc. Make sure you keep notes to record your observations! Materials: Eggs (at least 2) Markers to draw on a face (waterproof) Plastic container with top. Water (to fill the container) 14 Spinal Cord Refer to the diagram showing the relationship between spinal nerve roots and vertebrae to answer the question below. Diagram Showing the Relationship between Spinal Nerve Roots and Vertebrae Case Study of the Spinal Cord: Jim was driving to work when he was in a severe car accident. Somebody hit him from the behind while he was stopped at a red light. Instantly, he knew something was wrong with his body. He was taken to Lutheran General Hospital. It was at the hospital that the neurologist discovered he had damaged the posterior side of his spinal cord. Which would be more likely a result of his injury— paralysis or parethesia (loss of sensory input)? Explain your answer. __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ __________________________________________________________________________ 15 Special Senses Introduction Our bodies, via the nervous system, are sensitive to dozens of different stimuli both internal and external. The senses we are most familiar with are sometimes called the “special senses” and they include sight, hearing, smell, taste, and touch. Odoriferous (Olfaction) 1. Find the scent samples and see if you can identify some common “mystery” odors. A._________ B.__________ C.___________ D.__________ E.___________ 2. Look at the diagram of the nasal cavity at the right. A human olfactory tract is pictured. On the diagram, circle an olfactory receptor neuron. When we smell something, odor molecules come in contact with about 100 million olfactory neurons in the uppermost part of the nasal cavity. Each odor is chemically different in shape and is thought to uniquely “fit” into the olfactory membrane area. Why is cilia present in this membranous area? ______________________________________________________________________________________ 3. Olfactory Fatigue a. Close one nostril and smell oil on clove leaves until you can’t smell it anymore. b. Now, see if you can smell peppermint extract with your “fatigued” nose. c. Can you smell a different odor, if your nose is tired out from smelling another odor? Explain. __________________________________________________________________________________ d. Using the information in #2 above, why do you suppose your nose can get tired out on one odor, you can still smell another. __________________________________________________________________________________ 16 MMM….. GOOD (Gustation) The mouth contains a special sensory organ. What is it? ________________ Label the picture below of this organ. Then, identify the part that actually contains the sensory receptor. Label the diagram of it. Then, complete the lab. Organ Contains sensory receptor: _______________ Materials: Each student: 4 cotton-tipped applicators, 1 plastic taste solution dish, 1 taste map. Class sharing: bottles of bitter solution, sour solution, salty solution, and sweet solution Procedure: Part I 1. Rinse mouth out with water. 2. Pour a small amount of one of the taste solutions into the taste dish so there is enough to cover the bottom of the dish. 3. Dip a clean cotton-tipped applicator into the liquid. Drain the excess solution from the applicator by pressing it against the side of the dish. 4. Touch the applicator to the tongue of your partner in the regions outlined on the taste map. Tell your partner to place a plus (+) sign on the corresponding area of his/her taste map if he/she can sense the taste. If he/she cannot sense the taste, have him/her place a minus (-) sign in the appropriate place on the map. 5. After the four areas of the tongue have been tested with one solution, snap the applicator and discard it. 6. Exchange roles with your partner and repeat the test with the same solution. 7. Rinse your mouth with water. Also, rinse out the taste dish. 8. Repeat the procedure with each of the other three taste solutions. Back of Tongue Sweet Sweet Bitter Salty Sour Front of Tongue 17 Part II: Materials Per Group: 4 Dixie cups, each with one of the four mixtures of the unknowns, and eight straw sections. Procedure: 1. Label the four cups unknown #1, unknown #2, unknown #3, and unknown #4. DON”T SMELL YET! 2. The person who is going to taste the mixtures will close their eyes. 3. Have the taster hold their nose, take a straw section and a bit of the mixture and have the taster eat the mixture. Keep the nose closed the whole time. 4. The taster will try to identify the substance before releasing their nose and swallowing. Record the results in the following table. Use a “+” if the guess was correct and an “O” if incorrect. 5. The taster should rinse their mouth out between tastes. 6. Repeat for each of the remaining three mixtures in random order. And then switch roles. Record the results. 7. Next, have one partner close their eyes and have them try to identify the mixtures by smell. Do this in a random order. Switch roles and record all results. Mixture Unknown #1 Unknown #2 Unknown #3 Unknown #4 Taste no smell Smell Questions: 1. What are the unknowns? 2. What do the results indicate about the senses of smell and taste? Explain 3. While there are only four basic tastes, many flavors are experienced. How is this possible? 18 An Eye for an Eye….. (leaves the entire world blind) Label the special sensory organ below. What is it? ____________ Give the function of each of the following parts. Layers and coats o Sclera— Cornea— o Choroid— Ciliary body— Iris— o Retina— Rods (black & white) Cones (color) Fovea centralis Optic disc (no photoreceptor cells) AKA Blind Spot Cavities of the Eye o Anterior cavity— Filled with aqueous humor—clear, watery fluid o Posterior cavity— Filled with vitreous humor—soft, gelatin-like substance Helps with intraocular pressure to prevent collapse Glaucoma—too much intraocular pressure due to too much aqueous humor Muscles of the Eye 1. Extrinsic eye muscle— 2. Intrinsic eye muscle— Iris Ciliary body 19 Sheep Eye Dissection This lab should not take you more than 25 minutes. Follow all directions precisely! Remember to follow all lab safety rules. Materials: One sheep eye, dissecting kit, dissecting tray, paper towels, latex gloves, goggles, partner Part 1—External Observations 1. Note the eye’s shape. ________________________________ 2. Examine the pupil, iris, sclera, and cornea. 3. What does the texture of the eye feel like? _______________ Part 2—Internal Structures 1. Using the scalpel, very carefully remove the cornea from the eye. 2. Approximately how thick is the cornea? _________________ 3. What happens to the rest of the eye when you remove the cornea from it? __________________________________________ 4. Make a small incision in the ciliary body. 5. What happens when you do this? _________________________ 6. Remove the lens from the eye using scissors. Cut as close as possible to the muscles holding it in place. 7. Using the scalpel, very carefully shave the lens. What does this remind you of? What other “object” peels just like the lens does? ____________________________________________________ Part 3—Deep in the Eye 1. After removing the lens, what is exposed? ____________________ 2. Turn the eye upside down. What does the fluid coming out remind you of? ______________________ 3. What is it really? __________________________________ 4. Once all the fluid is removed, take a probe and lightly scrap the back of the retina. 5. What do you see? Look closely. Part 4—Clean-up 1. Clean all utensils. Wash with soap and water. Dry and return to kit. 2. Throw away all sheep parts. These go in the TRASH CAN! 3. Wash and dry the dissecting trays. Remove the mat and make sure all the fluids are washed out. 20 Vision: Image Formation As with any image being formed, it takes two or more rays from the same spot on an object to cross or appear to cross. In this case, looking at two rays from the candle flame, we find that the rays cross right at the location of the retina. This image would be in focus. It is important to notice that the real image of the candle is actually upside down on the retina. Your brain actually takes care of flipping it so it seems to be right side up. Some experiments that have been done involve people wearing some glasses that make everything seem upside down. After a few days, their brain flips the image. If they take the glasses off, their brain, after a delay, will flip it right side up again. Nearsightedness If the lens in the eye is too strong, or the cornea is too thick, or the eye is too long, it will cause the real image to get formed in front of the retina. This will cause a blurred image. We would call it nearsightedness (myopia). Uncorrected, nearsightedness basically means you can't see things from a distance. Since the problem is that the rays are converging too soon, we want to undo some of the convergence. Divergence undoes convergence. As a result, nearsightedness is usually corrected by placing a diverging lens in the front eye. Farsightedness - Draw the disorder in the box below. Refer to nearsightedness to help you. If the lens in the eye is too weak, or the cornea is too thin, or the eye is too short, it will cause the real image to get formed in beyond the retina. This will cause a blurred image. We would call it farsightedness (hyperopia). Uncorrected, farsightedness basically means you can't see things close up. Now, draw how to correct it in the box below. Since the problem is that the rays aren't converging soon enough, we need to increase the convergence. Farsightedness is usually corrected by placing a converging lens in the front eye. 21 Next, Use the mnemonic ‘racc’ to help you list the steps for the formation of vision. Briefly describe each step. R A C C Lastly, match the eye disorder with the proper explanation Myopia ____ Hyperopia ____ Presbyopia ____ Astigmatism ____ Cataract ____ Retinal detachment ____ Glaucoma ____ Analyze the special sensory organ below. A. Nearsightedness B. Farsightedness C. degeneration of accommodation D. clouding of lens E. Cornea or lens not uniformly curved F. pressure by aqueous humor G. could cause blindness What is it?_____________ What do the numbers represent?_________________ Explain this sensory process._______________________________________________________________________________________ _____________________________________________________________________________________________ _____________________________________________________________________________________________ _____________________________________________________________________________________________ _____________________________________________________________________________________________ _____________________________________________________________________________________________ 22 Hearing Lab 1. You will be performing four hearing tests on your laboratory partner today. These tests are: 1) auditory acuity test 2) sound localization test 3) Rinne’s test 4) Weber’s test 2. It will probably be necessary to have quiet surroundings to perform these tests with some degree of accuracy. 3. For the auditory acuity test, ask your laboratory partner to place a cotton ball in one ear, and ask to sit with eyes closed. a. Take two coins and tap them together lightly close to the open ear and slowly move it straight away from that ear. b. Ask your laboratory partner to indicate when the sound of the ticking can no longer be heard. Use a meter stick to measure the distance the clock/coins is away from that ear. Record your results in the following table. c. Repeat this procedure with the other ear. Ear Tested Audible Distance (in cm) Right Left d. What is the significance of the results of this test? 4. For the sound localization test, ask your laboratory partner to sit with eyes closed. a. Use the same coins tapped together, hold the coins somewhere within the audible range of your laboratory partner and at one of the locations listed below. b. Ask your lab partner to point to the location of the coins. c. Record your observations below. Actual Location of Clock/coins Reported Location Front of head Behind head Above head Right side of head Left side of head d. Repeat the procedure by moving the coins to another location and record your results below. e. Did your laboratory partner miss-identify any of the locations? Why? _______ 5. The Rinne’s test is used to assess for conduction deafness by comparing bone and air conduction. To conduct this test, do the following: a. Obtain a tuning fork from the front lab bench. Ask your laboratory partner to sit. b. Strike the tuning fork with a rubber hammer or with the heel of your hand to cause it to vibrate. c. Pace the handle of the fork against the mastoid process with the prongs of the fork pointed downward and away from the ear. The sensation of sound heard by your laboratory partner is actually due to conduction of vibrations through the bone rather than through the air as is normally done. d. If your laboratory partner cannot hear the sound, nerve deafness exists. 23 e. Ask your laboratory partner to tell you when he can no longer hear the sound. f. Quickly remove the tuning fork from the mastoid process and hold it close to the ear. Ask your laboratory partner if he can now hear the sound. If his hearing is normal, he will be able to hear the sound again. If not, then he has conductive impairment. Conductive impairment is usually the result of outer and middle ear defects. Hearing aids are frequently used to improve hearing in people with conductive impairment. g. Perform this test with the other ear and record your results below. Ear Tested Rinne’s test results (normal or impaired) Left Right 6. Weber’s test is used to distinguish conduction deafness or sensory deafness. To conduct this test, you will use your tuning fork and rubber hammer. a. Strike the tuning fork with the rubber hammer or the heel of your hand. b. Place the handle of the tuning fork against your laboratory partner’s forehead in the middle. c. d. Ask your laboratory partner if the sound appears louder in one ear or the other or if it is equally loud in both ears. If his hearing is normal, the sound will be heard equally in both ears. If some sensory deafness exists, the sound will be louder in the normal ear. This type of impairment involves the hearing organ (organ of Corte) or the cochlear nerve, and cannot be corrected by hearing aids. Record your results in the table below. Ear Weber’s test results (normal or impaired) Left Right e. Repeat the Weber’s test after placing a cotton ball in one of your laboratory partner’s ears. Usually the sound appears louder in the impaired ear because of filtering out of extraneous sounds. Record your results below. Ear Weber’s test results (normal or impaired) Left Right 24 What else does the regulate? _____________________ Let’s test it. Complete the lab below. The purpose of this exercise is to demonstrate the importance of vision in maintaining equilibrium. To perform this test, do the following: 1. Have your laboratory partner stand erect on one foot for one minute with his eyes open. Determine how steady or unsteady the subject is. 2. Now ask your laboratory partner to close his eyes and observe his ability to maintain equilibrium for one minute. What sensory organs allow for the maintenance of equilibrium when your laboratory partner’s eyes are open? (Hint: there are three.) What sensory organs provide this information when his eyes are closed? Römberg test The Romberg test is used to evaluate a person’s ability to integrate information from proprioceptors in muscles and receptors within the saccule and utricle to maintain static equilibrium. This information is relayed to the muscles used in maintaining posture to help maintain steadiness. A person who demonstrates unsteadiness with the eyes closed is considered to have a positive Römberg test. 1. Have your laboratory partner stand with his back to a chalkboard. 2. Turn on a bright light placed directly in front of your laboratory partner; his shadow should be cast on the chalkboard. 3. Ask your laboratory partner to look straight ahead for three minutes. Meanwhile, you will mark his degree of unsteadiness (side-to-side movement) with a piece of chalk. 4. Using a millimeter ruler, measure in cm the amount of side-to-side motion of your laboratory partner. Record your results in the table below. 5. Repeat this procedure, but have your laboratory partner close his eyes. Record your results in the table below 6. Now ask your laboratory partner to turn with one side to the chalkboard and stare straight ahead. Repeat the above procedure, and record your results below. 7. Ask your laboratory partner to close his eyes, and repeat the above procedure. Record your results below. Romberg test results: Test Conditions Maximal movement (in cm) Back toward board; eyes open Back toward board; eyes closed Side toward board; eyes open Side toward board; eyes closed Based upon your results, would you expect a person with his eyes closed to have a greater maximal movement than with his eyes open? Why? If a person had damage to the saccule and/or utricle, what would you predict would be the results of the Römberg test? Why? If a person had damage to the saccule and/or utricle, what would you predict would be the results of the Römberg test? Why? What is the difference between static and dynamic equilibrium? 25