EUGENIO RASALAN NUNEZ, JR.
10176 Ramona Drive, Spring Valley, CA 91977
Telephone No: 619.358.3781, Telefax No: 619.741.2607
E-mail address: er_nunez_jr@yahoo.com
State of California CLS License number: MTA 40493
EDUCATION:
Bachelor of Science
Major: Medical Technology
Far Eastern University – Dr. Nicanor Reyes
Medical Foundation, School of Medical Technology,
Morayta, Manila, Philippines
June 1987 – March 1991
EXPERIENCE:
Clinical Laboratory Scientist
Molecular Oncology
bioTheranostics, Inc.
9640 Town Centre Drive, Suite 200
San Diego, CA 92121
September 22, 2014 – March 12, 2015.
Performed DNA extraction using QIACube platform prior to library preparation, DNA quantification using
ABI 7500 Real-time PCR utilizing sybr green and ALK, KRAS and NRAS forward and reverse primers, DNA
library preparation and sequencing using Ion Torrent Next Generation Sequencing platform.
Performed daily, weekly and monthly QA/QC: reagent inventory, reagent qualification and reagent
verification, pre-amp and post-amp decontamination, bio analyzers and lab equipment daily and weekly
maintenance and calibration. Responsible for training and evaluation of CLS and Laboratory Associate and
updating and re-drafting SOPs and assay checklist.
Clinical Laboratory Scientist
Cell-Free Molecular Diagnostics, Molecular Microbiology & Molecular Oncology
Trovagene
11055 Flintkote Avenue
San Diego, CA 92121
(858) 952.7570
November 19, 2013 – October 21, 2014
Performed DNA isolation and purification using Qiagen platform. Performed Tr-DNA quantification using
Qubit 2.0 fluorometer, Droplet Generator and Reader. Performed testing for BRAF V600E mutations
determination using RainDrop Droplet Digital PCR: RainDrop Source and RainDrop Sense and used
QuantaSoft software in data analysis. Performed testing for HR-HPV using identification of the amplicon
using ABI 3130 and 3130XL Capillary Electrophoresis. Results out patients results for final review of Medical
Director. Performed daily, weekly and monthly QA/QC: pre-amp and post-amp decontamination, bio
analyzers and lab equipment maintenance, calibration. Participated in clinical validation for HR-HPV
detection assay and qualitative BRAF V600E mutation determination. Helped in SOP and checklist
revisions and monitoring QC trends and failures. Helped also with the training of new CLS.
EUGENIO RASALAN NUNEZ, JR.
Clinical Laboratory Scientist
Genetics
Millennium Laboratories
16981 Via Tazon
San Diego, CA 92127
(877) 451.3454
(858) 451.3535
June 11, 2012 – November 18, 2013
Performed pre-analytical and analytical testing and interpreting report and patient results to ordering
physicians. Reports to Director of Genetics for day to day clinical operations. Performed DNA isolation using
Hamilton Star Seires liquid handler using SSRB (Solid Surface Reversible Binding) technology for DNA
isolation. Performed normalization of DNA isolates using Hamilton MicroLab Starlet Liquid Handler.
Performed genotyping using Fluidigm Biomark HD RT PCR system platform, sequencing of isolated DNA
using ABI 3730XL platform, according to approved standard operating procedure. Handled quantified
isolated DNA using FilterMax Multi-Mode Microplate Reader employing PicoGreen Analysis platform.
Participated in technical and non-technical SOP drafting and revision.
Clinical Laboratory Scientist II
Module Lead, Multiplexing & Sequencing
Sequenom Center for Molecular Medicine
3595 John Hopkins Court
San Diego, CA 92121
(858) 202.9000
August 2011 – March 2012
Overseeing daily operations of T21 submodule - multiplexing and sequencing as well as leading submodule
core team on top of performing sequencing using Illumina HiSeq2000 platform. Responsible for quality
control monitoring of HiSeq2000 and cBot including basic to advance troubleshooting. Responsible for
updating and redrafting of SOPs in preparation for CAP and NY inspections as well as internal audit.
Routine data entry, data analysis and releasing of results. Reports daily to Clinical Director regarding day to
day operations and TAT.
Clinical Laboratory Scientist II
Molecular Genetics
Sequenom Center for Molecular Medicine
3595 John Hopkins Court
San Diego, CA 92121
(858) 202.9000
March 22, 2011 up to January 2013
Performed DNA extraction, prepared DNA libraries. Quantified DNA concentration using Caliper GX.
Normalized DNA concentration using Zephyr liquid handler. Processed PCR products for clustering using
C-bot (Illumina) and DNA sequencing using HiSeq (Illumina) testing for Trisomy 21 mutations.
Performed RhD determination using MALDI-TOF (matrix assisted laser desorption/ionization – Time of
Flight) mass spectrometer. Performed routine maintenance and calibration for MALDI-TOF, Caliper GX,
Zephyr, HiSeq and C-bot. Managed and participated in CAP proficiency testing program for molecular
laboratory CAP certification. Participated in technical and non-technical SOP drafting and revision. Facilitator
for continuing medical education among laboratory personnel.
Clinical Laboratory Scientist (Consultant)
Immunology & Immunochemistry
GenWay Biotech, Inc.
6777 Nancy Ridge Drive
San Diego, CA 92121
(858) 458.2846
January 31, 2011 up to October 30, 2013
Performed ELISA, immunochemistry along with assay validation for clinical trials involving cardiac markers,
infectious disease and tumor markers (e.g. CRP, CMV, HSP 60 and HSP 70, PSA, CA 15-3).
EUGENIO RASALAN NUNEZ, JR.
Clinical Laboratory Scientist
Molecular Genetics
Prognosys Biosciences, Inc.
505 Coast Blvd., Suite 310
La Jolla, CA 92037
(858) 459.2428, (858) 459.2429
October 6, 2008 – March 2, 2011
Performed DNA extraction, prepared DNA libraries, processed PCR products for clustering using cluster
station (Illumina) and DNA sequencing using Genome Analyzer (Illumina). Performed PAGE for clinical
testing (e.g. FLT3 and D835 mutations). Performed routine calibration and maintenance of cluster station
and genome analyzer. Assists in R&D projects, e.g. protein purification, scan microarrays using Scan
Arraylite Microarray Analysis System-GSI Lumonics, gel imaging and analysis. Other instruments used: ND
1000 and ND3300. Developed new laboratory techniques (e.g. wipe test) and updates on new procedures
and R&D related experiments. Daily monitoring of DNA contaminants and other amplicons that may
interfere with sequencing assay and other experiments. I helped in establishing clinical operations in the
molecular laboratory and was CAP certified after six months. Managed and participated in CAP proficiency
testing program for molecular laboratory CAP certification.
Clinical Laboratory Scientist I
Immunology, Immunochemistry
Prometheus Laboratories, Inc.
9410 Carroll Park Drive
San Diego, CA 92121
(888) 423.5227
December 17, 2007 – December 31, 2008
Performed ELISA, immunochemistry (e.g. IBD, Chron’s disease, ulcerative colitis & celiac disease), IFA
microscopy (anit-endomysial antibody), HPLC for detecting metabolites (e.g. 6 thioguanines & 6
mercaptopurines). Performed daily calibration and maintenance of HPLC and Tecan. Managed and
participated in CAP proficiency testing program for laboratory CAP certification.
Clinical Laboratory Scientist III
Molecular Cytogenetics
Biocept, Inc.
5810 Nancy Ridge Drive,
San Diego, CA 92121
(888) 332.7729, (858) 320.8200
November 7, 2007 – September 19, 2008
Performed cell separation, cell culture, sample processing, FISH analysis and image analysis (e.g. sex
chromosomal aneuploidies like Trisomy 21 & Turner’s syndrome, isolation of circulating malignant cells, like
prostate cancer cells and breast cancer cells) using CEE - Cell Enrichment and Extraction platform
combined attachment chemistry with mathematically modeled fluid dynamics and nanotechnology.
EUGENIO RASALAN NUNEZ, JR.
Clinical Laboratory Scientist I
Hematology & Oncology, Serology & Immunology, Clinical Chemistry
Quest Diagnostics Inc.,
33608 Ortega Highway, San Juan Capistrano, CA 92690
949.728.4000, 1.800.NICHOLS
September 12, 2005 – October 27, 2007
Performed qualitative, quantitative & RT-PCR using AB1 79000HTSequence Detection System, (e.g.
BCR/ABL kinase quantitative PCR, JAK2 V617F mutations qualitative PCR, FIP1L1-PDGFRA gene
rearrangement (del 4q12) RT-PCR, BCL gene rearrangement qualitative/quantitative PCR, c-kit mutation
analysis, T cell receptor gene rearrangement quantitative PCR, AML1-ETO t (8; 21) ratio quantitative RTPCR, ELISA (e.g. NMP22, IL2 receptor & IL6, VEGR, EGFR, HPV ELISA, cryptococcal antibody).
Performed clinical lab testing using Hitachi 717 (e.g. urine and CSF creatinine). Managed and participated
in CAP and NY proficiency testing program for laboratory CAP certification. Supervised and trained new
laboratory personnel.
Medical Technology Intern - Rotating Medical Technology Intern-in-training.
April 1990 – March 1991
Rizal Medical Center, Jose Reyes Memorial Medical Center
Responsible for performing and analyzing various laboratory procedures in bacteriology, blood banking and
hematology, histopathology, EKG & nuclear medicine, clinical chemistry and clinical microscopy. Assist the
Pathologist in performing autopsy. Responsible for collecting specimens like blood extraction (intravenous
and peripheral).
EXAMINATIONS:
Clinical Laboratory Scientist, State of California, USA, MTA 40493
January 2005
Philippine Medical Technology Licensure Examination, PRC 27151
March 1993
ORGANIZATION:
FEU-NRMF Medical Technology Alumni, Member
1991
LANGUAGES:
English (fluent written and spoken), Tagalog (fluent written and spoken), Chinese (can speak with difficulty).
PERSONAL: US Citizen.
VOLUNTEER EXPERIENCE AND CAUSES:
Community & Crime Patroller
Stonewall Citizens’ Patrol
Hillcrest and North Park, San Diego, CA
SPECIAL INTERESTS:
Travel, music, teaching.
REFERENCES: Available upon request.