BIO 101 Worksheet Metabolism and Cellular Respiration
Glycolysis True or false. If false, indicate why
1. ____ Glycolysis takes place in the cristae of mitochondria. This is false because glycolysis takes place in the
cytoplasm. Its oxidative phosphorylation that occurs in the cristae
2. __ An end product of glycolysis is pyruvate
3. ____ A 6 carbon sugar is oxidized in glycolysis
4. ___ CO2 is a waste product of glycolysis. This is false because CO2 is a product of the citric acid cycle.
5. ____ Glycolysis leads to fermentation in some bacteria and yeast
6. __ Glycolysis involves an energy pay-off and then an energy investment phase. False – investment before
payoff. 2 ATP are invested and 4 are produced for a net gain of 2 ATP in glycolysis
7. ____ A net of 4 ATP are produced in glycolysis. The net gain is 2 ATP for each molecule of glucose
8. ___ Glycolysis involves 10 steps tightly controlled by enzymes
9. __ Glycolysis is an exergonic process that releases energy
10. ____ Glucose is modified to form acetyl CoA for the citric acid cycle. Pyruvate is the substrate for the
acetylCoA reaction.
11. _____ Glycolysis does not use oxygen, it is anaerobic
12. ____ Glycolysis is the first step in cellular respiration
13. ____ It is likely that glycolysis was the method of ATP production in first organisms on Earth.
14. ____ Organisms that engage in glycolysis cannot engage in fermentation. The organisms that engage in
fermentation also perform glycolysis. The 2 are linked – see the figure in the text concerning NAD+ and
NADH.
The Citric Acid Cycle True or false. If false, indicate why
1. ____ The purpose of the citric acid cycle is to remove excess oxygen from cells. The purpose is to
generate electron carriers NADH and FADH2 and also to produce ATP
2. ____ The initial molecule in the citric acid cycle is acetyl-CoA
3. ____ The citric acid cycle occurs in the inner membrane of the mitochondria. The citric acid cycle
occurs in the matrix of the mitochondria
4. ____ 1 glucose molecule leads to 2 turns of the citric acid cycle and produce 2 ATP
5. ____ The citric acid cycle is a loosely controlled series of enzymatic steps. All steps of cellular
respiration are under tight control.
6. ____ All living organisms engage in the citric acid cycle. Only organisms that have a mitochondria can
engage in the citric acid cycle. Therefore, bacteria cannot.
7. ____ Carbon dioxide is produced in the citric acid cycle
8. ____ Electron carriers oxygen and water are produced in the citric acid cycle. The electron carriers are
NADH and FADH2.
9. ____ The citric acid cycle is the second step in cellular respiration
10. ____ The citric acid cycle is also called the tricarboxylic acid cycle. This is true, it is also called the
Krebs cycle.
Oxidative phosphorylation True or false. If false, indicate why.
1. ____ Oxidative phosphorylation occurs in the cytoplasm. This is false – it occurs in the inner membrane of
the mitochondria
2. ____ Oxidative phosphorylation is an aerobic process
3. ____ Ca+2 ions are pumped into the mitochondrial matrix during electron transport. The ions are H+. They
are pumped into the mitochondrial space between the inner and outer membrane. They are not pumped into
the matrix, but FROM the matrix.
4. ____ Electrons are donated by NADH and FADH2 and energy is harvested in a step wise fashion
5. ____ Water is produced in oxidative phosphorylation
6. ____ ATP is broken down to 34 ADP + 34 P in oxidative phosphorylation. ATP is synthesized in ox phos.
34 ADP are joined to 34P by the enzyme, ATP synthase, in an enzymatic reaction driven by the H+ ions
7. ____ ATP is synthesized by a ATP synthase
8. ____ ATP synthase requires H+ ions to operate
BIO 101 Worksheet Metabolism and Cellular Respiration
9. ____ The total amount of ATP produced by all cellular respiration activities is ~ 24. The total is 2 + 2+ 34
= 38 (approx.)
10. ____ Cellular respiration can be represented by: C6H12O6 + CO2 - O2 + H2O + energy. The correct
reaction is C6H12O6 + O2 - CO2 + H2O + energy
11. ____ Mitochondria are located in the plasma membrane of eukaryotic cells. Mitochondria are located in the
cytoplasm of eukaryotic cells.
12. ____ One cell may have thousands of mitochondria
13. ____ lipids, carbohydrates, and amino acids can serve as fuel for cellular respiration
Fermentation True or false. If false, indicate why
1. ____ Fermentation is linked to the citric acid cycle. No, fermentation is linked to glycolysis.
2. ____ Fermentation may produce ethanol
3. ____ Yeast and bacteria engage in fermentation
4. ____ Human cells can engage in fermentation
5. ____ Obligate anaerobes engage in fermentation
6. ____ Fermentation begins with pyruvate
7. ____ Fermentation does not produce CO2. Alcohol fermentation does produce CO2 as a waste product.
8. ____ Fermentation occurs in the cytoplasm
9. ____ Fermentation cannot produce ATP. This is true! Glycolysis produces 2 ATP per glucose. Fermentation
regenerates NAD+ so that glycolysis can continue
10. ____ Fermentation is linked to glycolysis