Part 1 - Get a Lab Appointment and Install Software:
Set up an Account on the Scheduler (FIRST TIME USING NANSLO):
Find the email from your instructor with the URL (link) to sign up at the scheduler.
Set up your scheduling system account and schedule your lab appointment.
NOTE: You cannot make an appointment until two weeks prior to the start date of this lab assignment.
You can get your username and password from your email to schedule within this time frame.
Install the Citrix software: – go to http://receiver.citrix.com and click
download > accept > run > install (FIRST TIME USING NANSLO).
You only have to do this ONCE. Do NOT open it after installing. It will work automatically when you go
to your lab. (more info at
http://www.wiche.edu/info/nanslo/creative_science/Installing_Citrix_Receiver_Program.pdf)
Scheduling Additional Lab Appointments:
Get your scheduler account username and password from your email.
Go to the URL (link) given to you by your instructor and set up your appointment.
(more info at http://www.wiche.edu/nanslo/creative-science-solutions/students-scheduling-labs)
Changing Your Scheduled Lab Appointment:
Get your scheduler account username and password from your email. Go to http://scheduler.nanslo.org
and select the “I am a student” button. Log in to go to the student dashboard and modify your
appointment time. (more info at http://www.wiche.edu/nanslo/creative-science-solutions/studentsscheduling-labs)
Part 2 – Before Lab Day:
Read your lab experiment background and procedure below, pages 1-24.
Submit your completed Pre-Lab Questions (page 6) per your faculty’s instructions.
Watch the Gas Chromatograph Control Panel Video Tutorial
http://www.wiche.edu/nanslo/lab-tutorials#GC
Part 3 – Lab Day
Log in to your lab session – 2 options:
1)Retrieve your email from the scheduler with your appointment info or
2) Log in to the student dashboard and join your session by going to http://scheduler.nanslo.org
NOTE: You cannot log in to your session before the date and start time of your appointment. Use
Internet Explorer or Firefox.
Click on the yellow button on the bottom of the screen and follow the instructions to talk to your lab
partners and the lab tech.
Remote Lab Activity
SUBJECT SEMESTER: ____________
TITLE OF LAB: Gas Chromatography
Lab format: This lab is a remote lab activity.
Relationship to theory (if appropriate): In this lab you will learn the underlying principles
behind the analytical technique of gas chromatography and learn some of the basic skills
involved in the interpretation of a gas chromatograph.
Instructions for Instructors: This protocol is written under an open source CC BY license. You
may use the procedure as is or modify as necessary for your class. Be sure to let your students
know if they should complete optional exercises in this lab procedure as lab technicians will not
know if you want your students to complete optional exercise.
Instructions for Students: Read the complete laboratory procedure before coming to lab.
Under the experimental sections, complete all pre-lab materials before logging on to the
remote lab, complete data collection sections during your on-line period, and answer questions
in analysis sections after your on-line period. Your instructor will let you know if you are
required to complete any optional exercises in this lab.
Remote Resources: Primary - Gas Chromatograph, Secondary – Pump and 5-component
solution.
CONTENTS FOR THIS NANSLO LAB ACTIVITY:
Learning Objectives........................................................................................ 2
Background Information ............................................................................... 2-6
Pre-lab Activities: Pre-lab Question 1 .......................................................... 7
Pre-lab Activities: Pre-lab Question 2 .......................................................... 7
Pre-lab Activities: Pre-lab Question 3 .......................................................... 7
Pre-lab Assignment – Setting Up Your Data Table ........................................ 7
Equipment ..................................................................................................... 8
Preparing for this NANSLO Lab Activity ........................................................ 8-9
Experimental Procedure ............................................................................... 9
Exercise 1: Iso-Thermal Trial ........................................................................ 9
Exercise 2: Ramping Trial ............................................................................. 9-10
Exercise 3: Ramping Trial ............................................................................. 10-11
Post Lab Thoughts/Questions ....................................................................... 11
1|Page
Last Updated May 27, 2015
CONTENTS FOR THIS NANSLO LAB ACTIVITY – CONT’D
Exercise 4: (Optional) Pressure Changes ...................................................... 11
Gas Chromatograph NANSLO Control Panel Instructions ............................ 12–25
Creative Commons Licensing ........................................................................ 25
U.S. Department of Labor Information ......................................................... 25
LEARNING OBJECTIVES:
After completing this laboratory experiment, you should be able to do the following things:
1. Compare and contrast different molecular structures for polarity and be able to rank
molecules in terms of polarity.
2. Apply the concept of polarity and intermolecular forces of attraction in predicting
retention times on a Gas Chromatograph (GC) column.
3. Improve upon given GC parameters and recommend new ones to obtain optimum
resolution of peaks
BACKGROUND INFORMATION
Polarity is the way molecules react with other molecules and interact with the world around
them is determined by the composition and arrangement of atoms within the molecule. One
particular property that has a large influence on molecule-molecule interactions is polarity.
Polarity arises out of a difference between the atoms within the molecule based on
electronegativity. For example, a molecule such as H2 does not have any polarity and is
considered nonpolar because the atoms within the molecule are the same (both are H), and
therefore there is no difference. The same would be true for Br2 or I2 or any other diatomic
molecule. However, some molecules, such as H2O, are polar, which we will explain in the
following paragraphs.
Electronegativity is the strength of the attraction of a particular atom to the electrons in a
covalent bond. The larger the electronegativity the stronger the attraction and the more tightly
the atom pulls on the electrons in a covalent bond. The most electronegative element on the
periodic table is Fluorine with a value of 4.0. As you move across the table towards Fluorine,
the electronegativity generally increases. Likewise, electronegativity generally increases as you
move up a group (column).
2|Page
Last Updated May 27, 2015
Figure 1: Illustration of how electronegativity increases as you move toward Fluorine.
The polarity of a bond within a molecule may be determined based on the electronegativity
difference between the elements that are bonded together. For example, in the bond between
carbon and fluorine shown below, fluorine is the more electronegative atom and therefore the
partial negative is on fluorine and the partial positive is on carbon. The delta symbol (δ)
indicates a partial charge.
Figure 2: Bond between carbon and fluorine.
The polarity of a molecule not only depends on the electronegativity differences (if any)
between the atoms in the molecule, but also on the 3-dimensional shape of the molecule. For
example, carbon dioxide (shown below on the left) has two polar bonds but the overall
molecule is nonpolar because the two bond dipoles cancel each other out. However, the water
molecule (shown below on the right) which also has two polar bonds is in fact polar because
the bond dipoles do not cancel out due to the tetrahedral electron geometry around the
oxygen atom. (Recall that there are two lone pairs of electrons on the oxygen atom).
3|Page
Last Updated May 27, 2015
Figure 3: The left image shows carbon dioxide with two polar bonds forming
a nonpolar molecule, and the right image shows a water molecule
with two polar bonds creating a polar molecule.
How polar a molecule is will also depend on the strength of the molecular dipole. For example,
the net dipole for dichloromethane is smaller than the net dipole in chloroform.
Intermolecular Forces of Attraction: The polarity of a compound influences how strongly the
molecules are attracted to each other. Polar compounds are capable of having dipole-dipole
interactions which are very strong. Nonpolar compounds can only have London dispersion
forces which are very weak.
Gas Chromatography: Chromatography separates compounds or particles based on a specific
physical property. There are several types of chromatography that are commonly used such as
thin layer chromatography, column chromatography, and gas chromatography. The device that
is used in this lab activity is called a “gas chromatograph” or simply a “GC”. In the gas
chromatography technique that will be used in this lab, molecules will be separated based on
their polarity. In every form of chromatography, there are two phases, a stationary phase and a
mobile phase. For the gas chromatography, the stationary phase is a column through which the
molecules will pass as they are carried through by the mobile phase which, in this case, is just
air. Often, light gases such as helium or hydrogen are used as the mobile phase.
The interaction with the column determines how long the molecule will take to get through the
column before being detected by the instrument. This causes different molecules to have
different retention times (the time it takes for the molecule to get through the column). The
stronger the interaction between the molecule and the column, the longer the molecule will
stay on the column and therefore come out at a later time. The weaker the interaction, the
faster the molecule will come out of the column. Molecules that are similar in polarity to the
column will have stronger interactions. In other words, a nonpolar compound will interact
more strongly with a nonpolar column and a polar compound will interact more strongly with a
polar column.
4|Page
Last Updated May 27, 2015
In addition to polarity, the molecule’s volatility (i.e. – boiling point) will impact the retention
time. There is a direct relationship between the strength of the intermolecular forces of
attraction and the volatility of a substance. The stronger the attraction between the molecules,
the less volatile a substance will be. The compound’s molar mass also contributes to the
volatility. Molecules that are larger are capable of having stronger intermolecular forces of
attraction due to greater magnitude of the temporary dispersion forces and therefore will be
less volatile. Substances with lower boiling points (more volatile) will vaporize more quickly
than substances with higher boiling points. Therefore, the more volatile molecules might start
moving down the column sooner than the less volatile molecules, essentially getting a headstart, depending on what the starting temperature of the column is. For example, if you start
the column out at a temperature that is at or above the boiling points of the chemicals, they
will all “flash boil” at the same time and start down the column simultaneously. Of course, even
if the column never reaches the boiling point of the chemical, it will still evaporate and move
down the column (just like water will evaporate at room temperature). It is the combination of
volatility and polarity which determines how quickly a molecule will move through the gas
chromatography column.
The result of all this is that different types of molecules can be separated from each other as
they move through the gas chromatography column if the parameters such as column
temperature and gas flow (pressure) are set correctly. In this activity, you will be attempting to
completely separate four different chemicals and determine which peaks in the gas
chromatogram correspond to which chemicals.
As an example, here are the chromatograms produced by three of the four individual chemicals
that you will be using in this lab activity as well as the chromatogram produced by running a
mixture of all three chemical compounds through the GC.
5|Page
Last Updated May 27, 2015
Chromatogram 1: Methanol
Chromatogram 2: Butyl Acetate
Chromatogram 3:
2-Butanone
Chromatogram 4: Mixture of All Three
Figure 4: Graphs of the chromatograms for methanol, butyl acetate,
butanone, and a mixture of all three.
Note that it is the order in which the compounds come off of the column that we are focusing
on in this introductory lab activity. There is a lot of other information that can be gained from
detailed analysis of a chromatogram. For example, the area under the peak for each compound
indicates the relative amount of that compound in the overall mixture. Also, the shape of the
peak can tell us some interesting things about the chemical compound that produced it.
However, these more advanced topics will be left for a future lab activity.
6|Page
Last Updated May 27, 2015
PRE-LAB ACTIVITIES
Pre-Lab Question 1:
Rank the following in order of increasing polarity:
Most polar: _____________________
Middle: ________________________
Least polar: ____________________ _
Pre-Lab Question 2:
Assuming two compounds have similar boiling points, will a nonpolar compound have a longer
or shorter retention time than a polar compound on a polar column?
Pre-Lab Question 3:
Which of the following molecules should have a shorter retention time on a nonpolar column?
Explain why.
Pre-Lab Assignment - Setting Up Your Data Table:
The compounds you will test in this experiment are methanol, butyl acetate, isopropanol, and
2-butanone. Set up a data table with the Lewis structure, boiling point, and molar mass of
each.
Based on the information presented in the Background section, predict the order in which
these chemicals will come through the GC column under optimal conditions for separation.
7|Page
Last Updated May 27, 2015
EQUIPMENT:




Paper
Pencil/pen
Computer with Internet access (for the remote laboratory and for data analysis
Remote: Gas chromatograph and pumping system to deliver chemicals
PREPARING FOR THIS NANSLO LAB ACTIVITY:
Read and understand the information below before you proceed with the lab!
Scheduling an Appointment Using the NANSLO Scheduling System
Your instructor has reserved a block of time through the NANSLO Scheduling System for you to
complete this activity. For more information on how to set up a time to access this NANSLO lab
activity, see www.wiche.edu/nanslo/scheduling-software.
Students Accessing a NANSLO Lab Activity for the First Time
For those accessing a NANSLO laboratory for the first time, you may need to install software on
your computer to access the NANSLO lab activity. Use this link for detailed instructions on
steps to complete prior to accessing your assigned NANSLO lab activity –
www.wiche.edu/nanslo/lab-tutorials.
Video Tutorial for RWSL: A short video demonstrating how to use the Remote Web-based
Science Lab (RWSL) control panel for the air track can be viewed at
http://www.wiche.edu/nanslo/lab-tutorials#GC.
In addition, watch these other videos to help you understand how gas chromatography works.
VIDEO 1: An animation of the dynamic process of how polar and nonpolar molecules
are physically separated on a gas chromatography column (2:29 minutes):
https://www.youtube.com/watch?feature=player_embedded&v=g1W7cZDad10
VIDEO 2: Gas Chromatography Remote Web-based Science Lab (RWSL) Lab Interface Tutorials:
https://www.youtube.com/watch?feature=player_embedded&v=iAMIRoME1qg
VIDEO 3: In-depth look at the principles of gas chromatography (2:21 minutes):
https://www.youtube.com/watch?feature=player_embedded&v=q0pM-k0SvOQ
VIDEO 4: Animation of how the peaks are produced on the chromatogram (9 seconds):
https://www.youtube.com/watch?feature=player_embedded&v=OnQglXDvzTc
8|Page
Last Updated May 27, 2015
NOTE: Disregard the conference number in this video tutorial.
AS SOON AS YOU CONNECT TO THE RWSL CONTROL PANEL: Click on the yellow button at the
bottom of the screen (you may need to scroll down to see it). Follow the directions on the pop
up window to join the voice conference and talk to your group and the Lab Technician.
EXPERIMENTAL PROCEDURE
Once you have logged on to the remote lab system, you will perform the following laboratory
procedures. See Preparing for the Gas Chromatography NANSLO Lab Activity below.
Exercise 1: Iso-Thermal Trial
4 compounds: Methanol, butyl acetate, isopropanol, and 2-butanone.
Trial #1: Run the mixture of the 4 compounds isothermally at the following profile:
o
o
o
o
o
o
o
Start temp = 90°C
Hold time = 1 MIN (HOW LONG TO STAY AT THE START TEMP)
Ramp rate = 0 °C/MIN
Final temp = 90°C
Hold time = 5 MIN (HOW LONG TO STAY AT THE FINAL TEMP)
Total Time = 6 MIN
Pressure = 7 KPA
1. What do you notice about the chromatogram that is produced? Are all the compounds
separated from each other?
2. Export a copy of the chromatogram (graph) and paste it into a document on your
computer. Insert this graph into your report.
Exercise 2: Ramping Trial
Trial #2: Run the mixture of the 4 compounds while ramping the temperature up.
o
o
o
o
o
o
o
9|Page
Start temp = 70°C
Hold time = 1 min
Ramp rate = 10°C/min
Final temp = 90°C
Hold time = 3 min
Total Time = 6 min
Pressure = 7 kPa
Last Updated May 27, 2015
1. What do you think will be different about this run?
Prediction:
2. What do you notice about the chromatogram that is produced? Are all the compounds
separated from each other?
3. How does the result compare with your prediction?
4. Export a copy of the chromatogram and paste it into a document on your computer.
Insert this graph into your report.
Exercise 3: Ramping Trial
Trial #3 and beyond: Improve the separation of the peaks from the last two runs by varying
only the ramp rate and starting and ending temperatures for your next 2 runs. Keep the
pressure at 7 kPa and keep the total length of time 10 minutes or under. Make a data table for
your parameters and observations. Your goal is to completely separate all four compounds
from each other.
Here are the limits for the various settings in the GC profile for the specific GC we are using:
Minimum Temperature: 30°C
Maximum Temperature: 160°C
Minimum Ramp Rate: 0°C/min
Maximum Ramp Rate: 10°C /min
Minimum Pressure: 1 KPa (above room pressure)
Maximum Pressure: 19 KPa (above room pressure)
Choose some profile settings that you think will give you even better separation of the peaks:
Trial #3:
o
o
o
o
o
o
o
10 | P a g e
Start temp = ___ °C
Hold time = ___ min
Ramp rate = ___ °C/min
Final temp = ___ °C
Hold time = ___ min
Total Time = ___ min
Pressure = 7 kPa
Last Updated May 27, 2015
1. Export a copy of the chromatogram and paste it into a document on your computer.
Insert this graph into your report.
2. Which of the four peaks represents the isopropyl alcohol?
3. How does the boiling point of isopropyl alcohol compare with the boiling point of the
other three compounds?
4. Explain why the isopropyl alcohol peak appears where it does in the gas chromatogram.
POST LAB THOUGHTS/QUESTIONS
1. If you ran the same mixture of compounds through a polar GC column, assuming
optimal conditions, in what order would they appear at the end of the separation?
2. Draw a picture of what you think the chromatogram would look like.
Exercise 4: (Optional) Pressure Changes
*Optional Trials: What impact do you think changing the pressure in the GC column will have?
Make a prediction and repeat one of your trials from above using a different pressure. Export a
copy of the chromatogram and paste it into a document on your computer. Insert this graph
into your report.
Prediction:
Evidence:
Conclusion:
11 | P a g e
Last Updated May 27, 2015
GAS CHROMATOGRAPH NANSLO CONTROL PANEL INSTRUCTIONS
The Remote Web-based Science Lab (RWSL) gas chromatograph is controlled remotely by using
a web interface as shown below. This NANSLO control panel allows you to control every
function of the gas chromatograph just as if you were sitting in front of it.
Figure 5: Remote Web-based Science Lab (RWSL) GC Lab Interface.
Communicating with your Lab Partners
As soon as you have accessed this lab interface, click on the “Voice Conference” yellow button
(you may need to scroll down to see it) to view instructions for communicating with your lab
partners and with the Lab Technicians. Only one person can be in control of the equipment at
any one time so talking together on a conference line helps to coordinate control of the
equipment and creates a more collaborative environment for you and your lab partners.
12 | P a g e
Last Updated May 27, 2015
Gaining Control of the Gas Chromatographer (GC)
Right click anywhere in the grey area of the lab interface and choose “Request Control of VI”
from the dialogue box that appears when multiple students are using the GC at the same time.
After you request control, you may have to wait a short time before you actually receive control
and are able to use the features on this lab interface.
Figure 6: Selecting "Request Control of VI"
Releasing Control of the Emission Spectroscopy Apparatus
To release control of the GC so that another student can use it, right click anywhere in the grey
area of the lab interface and choose "Release Control of VI" from the dialogue box that
appears.
Figure 7: Selecting "Release Control of VI"
13 | P a g e
Last Updated May 27, 2015
GC View Window
The Image View Window displays the real-time video feed from a digital camera focused on the
GC, the syringe, and the robotic pumping system.
Figure 8: Image View Window
Camera Present Positions and Pan-Tile-Zoom Controls
Several camera preset positions have been programmed for use with this lab interface. Use
these to look more closely at the equipment or to view what is happening in the lab itself. Preset position 2 for example allows you to view the screen on the GC that tells you the current
temperature of the column.
Figure 9: Camera Preset Positions
14 | P a g e
Last Updated May 27, 2015
The four arrows used to pan and tilt allow you to move the camera right to left and up and
down. The two zoom buttons allow you to zoom in to see a closer look at the equipment such
as shown in Figure 10 or zoom out to view more of the room.
Figure 10: Pan, Tilt & Zoon controls
Using the Cameras to View the GC Apparatus
There are two digital cameras set up for the GC lab. First, we'll describe the options when
"Camera Selection 1" is available.
Camera Selection = 1
This camera allows you to see what is going on in the lab and on the GC apparatus itself. To
determine what Camera Presets are available when Camera 1 is being used hover over the
preset buttons and a pop-up menu will appear showing the views, e.g. 1 – Full view. Use
Camera 1, Camera Preset 2 to see the screen on the GC itself.
For each camera preset view, additional camera options are available when Camera 1 is being
used (see
Figure 11.)
1. Use the up and down arrows to tilt the camera up or down.
2. Use the right and left arrows to pan right or left.
3. Use the left "Zoom OUT" arrow and right "Zoom IN" arrow to zoom out and in.
15 | P a g e
Last Updated May 27, 2015
Figure 11: Pan-Tilt-Zoom Camera Control
Camera Selection = 2
This camera allows you to see a closeup of the needle that injects the sample compound into
the GC. To determine what Camera Presets are available when Camera 2 is being used, hover
over the preset buttons and a pop-up menu will appear showing the views.
Make sure Camera 2, Camera Preset 2 is selected to view the needle itself.
Setting Up the GC Run Profile
Using the parameters set out in your lab procedure, change the profile settings using the GC lab
interface (highlighted in the red box). Once the profile has been defined, select the “Submit
Profile” button.
WARNING: Once you click Submit Profile, you cannot stop the process! If possible, have your
lab partners double check your settings before you submit them.
Figure 12: GC Run Profile Settings
16 | P a g e
Last Updated May 27, 2015
Running the GC Profile
Camera 1, Camera Preset 2 shows the screen on the GC itself which tells you the current
temperature of the column.
Figure 13: Camera 1, Camera Preset 2 shows GC temperature.
The “GC Status” field will indicate when the GC has reached the proper pressure and
temperature settings. At that point, the “Inject Needle” message appears in the GC Status
window. When the “Insert Needle” button is clicked, it causes the robot to move the pump
forward and insert the needle into the GC column. Select Camera 2, Camera Preset 2 to view
this needle.
Figure 14: Select the “Insert Needle” button.
17 | P a g e
Last Updated May 27, 2015
After the needle has been inserted, the “Input and Collect” button is available. When this
button is selected, the robotic pumping system will deliver the correct amount of sample
compound. The needle will be withdrawn from the column automatically.
Figure 15: Select the “Inject and Collect” button.
Data is now being generated.
Figure 16: GC is running data.
18 | P a g e
Last Updated May 27, 2015
Viewing the Data Generated by the GC in a Graphic Format (Chromatogram)
Select the “Graph” tab on the lab interface to view the data being generated. At first, the data
on the chromatogram will be noisy as shown in Figure 17. This will smooth out as soon as the
chemicals begin to work their way out of the end of the GC column and impact on the detector.
Figure 17: Data is “Noisy”
When this happens, you will see a dramatic change in the scale on the left side of the
chromatogram as shown in Figure 18. It often takes 5 to 6 minutes for the run to occur from
the beginning to the end of the column. You will see separate peaks for separate chemicals as
they exit the chromatograph column.
Figure 18: Real data is being captured.
19 | P a g e
Last Updated May 27, 2015
Analyzing the Data Generated by the GC (Chromatogram)
Once the chromatogram is completed, you want to analyze it to determine where the peaks are
located and perhaps their intensity. You can do this by clicking on the “Cursor” button. The
cursor will appear in the center of the chromatogram. Click and drag the cursor to a peak on
the chromatogram. The location of the peak in time (x-axis) and the amplitude of the peak (yaxis) are displayed in the Cursor box. See Figure 19.
Figure 19: Peak in time and amplitude based on area of chromatogram selected.
Manipulating the View on the Chromatogram
There are several tools available to you when viewing the chromatogram. The tools most often
used are:
1. The “Cursor” button (see Figure 19 above) displays a cursor in the center of the
chromatograph that can be moved around.
2. When selecting the center button on the small toolbar at the bottom right corner of the
chromatogram additional tools are visible. See Figure 20.
20 | P a g e
Last Updated May 27, 2015
Figure 20: Tool Options for Chromatogram.
The two tools most frequently used are:
a. The “zoom in” button allows you to select an area of the chromatogram that you
want to examine more closely. Move the magnifying glass displayed within the
chromatogram when the “zoom in” button is selected to the location where you
want a closer look. See Figures 21-22.
Figure 21: Zoom in to get a closer look at the chromatogram.
21 | P a g e
Last Updated May 27, 2015
Figure 22: Magnifying glass available
When you use the zoom in tool, the cursor sometimes disappears. If this
happens, click on the “Cursor” button to turn the cursor off and click it again to
turn it back on. The magnifying glass will reappear in the middle of the screen.
Figure 23: Click the “Cursor” button twice to see the magnifying glass.
22 | P a g e
Last Updated May 27, 2015
b. The chromatogram will always zoom out to its fullest display when the “zoom
out” button is selected. See Figure 24.
Figure 24: Zoom out to get a view farther away of the chromatogram.
3. After using the “Zoom In” and “Zoom Out” tools, you may have to select the Cursor
Control tool – the left button on the small toolbar at the bottom right corner of the
chromatogram. The cursor will again appear in the center of the chromatogram. See
Figure 25.
Figure 25: Cursor Control Button
23 | P a g e
Last Updated May 27, 2015
Exporting the Chromatogram Data
You can export to your clipboard a graph image of the chromatograph or the data and paste it
into a document on your computer. Using the drop-down menu, select what you want to
capture –graph image or graph data. Next, open up the document that you will paste the
captured information onto. For example, if you selected “Graph Image,” you could open Word
and select “Paste.” The image would be placed where your cursor is on the document. If you
selected “Graph Data,” you could open a spreadsheet such as Excel and paste the data into the
spreadsheet.
Figure 26: Exporting image or data to clipboard.
Beginning a New Exercise with a Different GC Profile
When you have completed your data collection for an exercise, you can click on the “Profile”
tab and change the settings for your next run.
24 | P a g e
Last Updated May 27, 2015
Equipment Used for the GC Lab
NANSLO uses the Vernier Mini GC for this lab. To see how the Vernier Mini Gas Chromatograph
is used to collect data, see http://verniervideos.s3.amazonaws.com/training_html5/mp4/Intro_to_Gas_Chromatography.mp4. Note,
that the description of how the data is collected in this video differs from this lab only with
respect to location of collection. In the Vernier video, a computer attached to the GC collects
the data. In this NANSLO lab activity, students use the NANSLO lab activity control panel to
capture that data remotely.
For more information about NANSLO, visit www.wiche.edu/nanslo.
All material produced subject to:
Creative Commons Attribution 3.0 United States License 3
This product was funded by a grant awarded by the U.S.
Department of Labor’s Employment and Training Administration.
The product was created by the grantee and does not necessarily
reflect the official position of the U.S. Department of Labor. The
Department of Labor makes no guarantees, warranties, or
assurances of any kind, express or implied, with respect to such
information, including any information on linked sites and
including, but not limited to, accuracy of the information or its
completeness, timeliness, usefulness, adequacy, continued
availability, or ownership.
25 | P a g e
Last Updated May 27, 2015