Supplementary Data
Methods
Quantification of cell orientation
In calculating the orientation of cells, 80 cells from 3 independent experiments were
counted for each data set. The orientation angle β is defined as the angle between the
long axis of the cell and the line perpendicular to the EF. The orientation angles were
plotted as box plots. If cells did not show significant alignment in response to EF
stimulation, the bar was long with an average value of around 45°. On the contrary, if
cells aligned perpendicularly to applied EF, the bar would be shorted with an average
value close to 0°
Figure Legends
Fig S1.
Scatter plots of NIH 3T3 fibroblasts after being stimulated by EF and/or β-lapachone.
After tracing all cells for 2 hours, their final positions were plotted on a circular graph.
The radius of each circle is 100 μm. 80 cells from 3 independent experiments were
counted in each plot.
Fig S2.
The box plot showing the alignment of NIH 3T3 fibroblasts under EF and/or
β-lapachone stimuli. The rotation angles β of 80 cells from 3 independent experiments
were measured in each condition. ***: Significant difference from unstimulated
control (p<0.01).
Fig S3.
Scatter plots of NIH 3T3 fibroblasts after being stimulated by EF and/or α-tocopherol.
After tracing all cells for 2 hours, their final positions were plotted on a circular graph.
The radius of each circle is 100 μm. 80 cells from 3 independent experiments were
counted in each plot.
Fig S4.
The box plot showing the alignment of NIH 3T3 fibroblasts under EF and/or
α-tocopherol stimuli. The rotation angles β of 80 cells from 3 independent
experiments were measured in each condition. ***: Significant difference from
unstimulated control (p<0.01).