PAPER #2: EMBARGOED PRESS RELEASE
STRICTLY UNDER EMBARGO UNTIL 12:00PM NOON ET (US) ON THURSDAY, DECEMBER 10,
2015
Region(s) of Interest: United States, Illinois
Institution(s): University of Chicago
Bacteria engineered with synthetic circadian clocks
Many of the body's processes follow a natural daily rhythm or socalled circadian clock, so there are certain times of the day when
a person is most alert, when the heart is most efficient, and when
the body prefers sleep. Even bacteria have a circadian clock, and
in a December 10 Cell Reports study, researchers designed
synthetic microbes to learn what drives this clock and how it
might be manipulated.
"The answer seems to be especially simple: the clock proteins
sense the metabolic activity in the cell," says senior author
Michael Rust, of the University of Chicago's Institute for
Genomics and Systems Biology.
"This is probably because cyanobacteria are naturally photosynthetic--they're actually responsible for a large
fraction of the photosynthesis in the ocean--and so whether the cell is energized or not is a good indication of
whether it's day or night," he says. For photosynthetic bacteria, every night is a period of starvation, and it is
likely that the circadian clock helps them grow during the day in order to prepare for nightfall.
To make their discovery, Rust and his colleagues had to separate metabolism from light exposure, and they did
this by using a synthetic biology approach to make photosynthetic bacteria capable of living on sugar rather than
sunlight.
"I was surprised that this actually worked--by genetically engineering just one sugar transporter, it was possible
to give these bacteria a completely different lifestyle than the one they have had for hundreds of millions of
years," Rust says. The findings indicate that the cyanobacteria's clock can synchronize to metabolism outside of
the context of photosynthesis. "This suggests that in the future this system could be installed in microbes of our
own design to carry out scheduled tasks," he says.
In a related analogy, engineers who developed electrical circuits found that synchronizing each step of a
computation to an internal clock made increasingly complicated tasks possible, ultimately leading to the
computers we have today. "Perhaps in the future we'll be able to use synthetic clocks in engineered microbes in
a similar way," Rust says.
Other researchers have shown that molecules involved in the mammalian circadian clock are also sensitive to
metabolism, but our metabolism is not so closely tied to daylight as the cyanobacteria's. Therefore, our bodies'
clocks evolved to also sense light and dark.
"This is presumably why, in mammals, there are specialized networks of neurons that receive light input from
the retina and send timing signals to the rest of the body," Rust explains. "So, for us it's clearly a mixture of
metabolic cues and light exposure that are important."
The bacteria that live inside of our guts, however, most likely face similar daily challenges as those experienced
by cyanobacteria because we give them food during the day when we eat but not during the night. "It's still an
open question whether the bacteria that live inside us have ways of keeping track of time," Rust says.
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The work was supported by a Burroughs-Wellcome Career Award at the Scientific Interface and by the National
Institutes of Health.
Cell Reports, Pattanayak et al.: "Controlling the Cyanobacterial Clock by Synthetically Rewiring Metabolism"
http://dx.doi.org/10.1016/j.celrep.2015.11.031. Link to the paper at: http://www.cell.com/cellreports/abstract/S2211-1247(15)01346-7
Related Files
• This Dropbox contains a PDF of the paper proof (may be manuscript due to a delay with proofs) and a video of
cyanobacteria (The yellow glow is the expression of the yellow fluorescent protein under control of the clock.
Credit: Michael Rust, PhD) : https://www.dropbox.com/sh/aquwqhtktzdshsq/AADtOWukeQSejoQN5cvrGDLa?dl=0
Author Contact:
Michael Rust, PhD
mrust@uchicago.edu
+1 (773) 834-1463
Media Contact:
Kevin Jiang
Science Writer & Media Relations Specialist
+1 773-795-5227 (o)
+1 404-819-3247 (c)
Kevin.Jiang@uchospitals.edu