ELISA kits for veterinary diagnostics
1. Enzootic bovine leucosis. iELISA kit and ELISA 3rd generation kit for
detection antibody to BLV in bovine serum.
Bovine leukemia virus iELISA and ELISA 3rd generation kit is commercially
available. The BLV ELISA 3rd generation kit of LT Biotech is used to detect the
presence of Anti-BLV antibodies in serum more efficiently. It is purified recombinant
virus protein conjugated with horse radish peroxidase.
All the wells of the microplate are coated with the purified recombinant virus protein
too. Serum sample is incubated together with conjugate.
2. Brucellosis. iELISA kit and cELISA kit for detection antibody to
B.abortus in animal serum.
Brucellosis is a worldwide zoonosis caused by infection with the bacterial genus
Brucella. These organisms, which are small aerobic intracellular coccobacilli, localize in
the reproductive organs of host animals, causing abortions and sterility. They are shed
in large numbers in the animal's urine, milk, placental fluid, and other fluids. Exposure to
infected animals and animal products causes brucellosis in humans.
Indirect ELISA kit and competitive ELISA kit are developed to detect antiBrucella antibodies in animals serum samples in screening
tests as well as
confirmatory ones. Tests are suitable for screening herds and individual animals.
3. Blutongue. iELISA kit for detection antibody to BTV in animal serum.
Bluetongue virus (BTV) infection involves domestic and wild ruminants such as
sheep, goats, cattle, buffaloes, deer, most species of African antelope and various other
Artiodactyla as vertebrate hosts. This non-contagious, insect-borne viral infection is in
apparent in the vast majority of infected animals but causes fatal disease in a proportion
of infected sheep, deer and wild ruminants.
Bluetongue virus is endemic in most parts of Africa and control measures are
influenced by a number of factors including climatic conditions, susceptibility of animals,
presence of reservoir hosts, occurrence of Culicoides and the serotype incriminated in a
particular locality.
Twenty-four different serotypes have been identified and the ability of each strain to
cause disease varies considerably. The severity of disease varies among different species
with symptoms being most severe in sheep resulting in deaths, weight loss and disruption
in wool growth. In highly susceptible sheep, morbidity can be as high as 100%. Mortality
averages from 2-30% but can be as high as 70%. Cattle often have a higher infection rate
than sheep and demonstration and severity of clinical signs varies depending on the strain
of virus. Currently circulating BT virus in Northern Europe is epidemiologically significant
because of demonstration of clinical signs in cattle.
the insect host
4.
Tests allow early detection of antibodies to BTV after infection of
cattle, sheep, goat and cloven-hoofed ruminant.
5. Foot and Mouth disease. iELISA kit for detection FMD virus antibody in
animal serum. For differention vaccinated animals (DIVA).
A highly contagious disease.
Foot and mouth disease (FMD) is the most contagious disease of mammals and
has a great potential for causing severe economic loss in susceptible cloven-hoofed
animals.
Foot and Mouth disease antibody ELISA test kit is used for the detection the
antibody in animal serum samples. It allows to assess the immunity conditions against
Foot and Mouth disease in the farm. It is suitable to investigate the epidemiology of the
Foot and Mouth disease. The differention of vaccinated animals is carried out according
"DIVA" (Differentiating Infected from Vaccinated Animals) strategy.
6. Trichinosis. iELISA kit for detection antibody in swine and horse serum
Indirect ELISA kit is the most commonly used method to detect Trichinella infection
in vital diagnostics of swine and horse serum samples. iELISA kit reveals an
antibody immediately on the 14th day after invasion.
7. Porcine Reproductive & Respiratory Syndrome (PRRS) . iELISA for
detection PRRS virus antibody in swine serum.
This kit based on ELISA is used to carry out rapid diagnosis and detection of PPRS
(Porcine Reproductive and Respiratory Syndrome) antibody in swine serum. The
recombinant protein NP is applied as coating antigen. The antibody of PPRS virus
reacts with the coated antigen, after adding of enzyme labeled conjugate. TMB is used
for coloration. The result is measured photometrically. The absorbance value is
proportional to the PRRS antibody contained in the sample, and the result can be
determined comparing standard positive or negative serum samples.
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8. Rift Valley fever. iELISA kit for detection antibody to mosquito-borne
bunyavirus in animal serum.
Rift Valley fever (RVF) is anacute viral disease that can cause severe
disease in domestic animals (such as buffalo, camels, cattle, goats and sheep) and
humans. Disease in these species is characterized by fever, severe illness,
abortions, and a high morbidity and mortality rate. The virus which causes RVF
belongs to the genus Phlebovirus in the family Bunyaviridae. Many of the related
Bunyaviridae viruses can cause fever and encephalitis. Another commonly known
Bunyaviridae virus is the Hantavirus.
The disease also results in significant economic losses, death and abortion
among RVF-infected livestock.
Indirect ELISA kit may confirm the presence of specific antibodies to the virus.
9. Peste des petits ruminant (PPRV). iELISA kit for detection antibody to
Morbillivirus in sheep and goats serum.
Peste des petits ruminants (PPR), also known as «goat plague», is a viral disease of
goats and sheep characterized by fever, sores in the mouth, diarrhea, pneumonia, and
sometimes death. It is caused by a morbillivirus in the family of paramyxoviruses, that is
related to rinderpest, measles and canine distemper. Cattle and several wild ruminants
have been infected most often experimentally, but goats and sheep are the usual
targets.
PPR occurs in Africa in countries lying between the Equator and the Sahara, in the
Arabian Peninsula, throughout most of the Near East and Middle East, and in south-west
Asia.
This multi-species kit includes sheep and goat serum and plasma. It is highly specified
and sensitive.
10.Contagious bovine pleuropneumonia (CBPP). iELISA kit for detection
antibody to Mycoplasma SC in bovine serum.
Contagious bovine pleuropneumonia (CBPP) is widespread in Africa and in other
regions of the world. This disease is particularly important in the semi-arid, sub-humid
and arid zones of tropical Africa, but CBPP incidence seems to be increasing in some
parts of East Africa. The epidemiology of CBPP is characterised by the occurrence of
sub-acute and symptomless infections, and the persistence of chronic carriers. Spread
of the disease is associated with cattle movement.
The incubation period for contagious bovine pleuropneumonia can be three weeks to
six months. After experimental inoculation into the trachea, the clinical signs appear in 2
to 3 weeks.
An indirect ELISA, is based on specific and strongly antigenic recombinant peptide of the
lipoprotein LppQ from Mycoplasma mycoides subsp. It is designed for serodiagnosis and
monitoring of contagious bovine pleuropneumonia.
11. Pasteurellosis. iELISA kit for detection antibody to Pasteurella multocida
in animal serum.
12.Leptospirosis. iELISA kit for detection Leptospirosis in animal serum.
An indirect ELISA kit is developed on the basis of recombinant antigen to detect anti-
leptospiral antibodies in animal serum.