Table 2. Taxonomy assignment of 2d reads derived from 16S rDNA sequencing using
MinIONTM.
Genus
assignment
Bacillus
Escherichia
Streptococcus
Lactobacillus
Staphylococcus
Rhodobacter
Clostridium
Listeria
Cronobacter*
Yersinia*
Citrobacter*
Alteromonas*
Burkholderia*
Sulfitobacter*
Total assigned
a
At 80%
identity
(SINA)
3
61
69
2
37
4
0
0
0
0
0
0
0
0
176/601
At 70%
identity
(SINA)
3
93
96
2
55
8
2
0
1
0
0
0
0
0
260/601
At 60%
identity
(SINA)
4
103
111
3
60
10
2
1
1
1
1
1
1
1
300/601
Species assignment (Blasta)
B.cereus
E.coli, E. fergusonii,Shigellaflexneri
S.mutans, S. gordonii, S. agalactiae
L.gasseri, L. fornicalis
S.epidermidis, S. capitis, S. aureus
R.sphaeroides
N/A
N/A
N/A
N/A
C. koseri*, C. muytjensii*
N/A
N/A
N/A
172/601
Blast searching against the NCBI bacterial 16S database. Valid assignments were based on
E-values < 1e-03 and alignments larger than 800bp. N/A means no species was found for the
respective genus. Species matching with those present in the mock community are
underlined and in bold. Rows with genera, species, and read counts highlighted with
asterisk (*) indicate they are not expected to be present in the mock community.