CAF-EMIF
Rue du Bugnon 27
1005 Lausanne
Switzerland
+41 (0)21 314 6886
+41 (0)21 314 7977
www.unil.ch/caf/emif
caf.emif@chuv.ch
REQUEST FOR MICROSURGERY SERVICES
GENERAL INFORMATION
Investigator:
Date of request:
Department/Division :
Address:
Phone:
1.
Fax:
Email:
State specific question to be addressed and a brief description of the project:
2.
Specify genetic background, strain, transgenic or knockout:
3.
Number of animals:
4.
Authorization number (must be obtained prior to study) :
SERVICES
1.
Check Services Requested
TRANSVERSE AORTIC CONSTRICTION (TAC)
Operative placement of constriction on transverse aorta – between the innominate and
left common carotid artery. Constriction routinely done around a 27 gauge needle, but
can be altered according to mouse weight and experimental protocol. Includes
recording mouse weight and echocardiography at study end – usually one week unless
otherwise requested.
2.
MYOCARDIAL INFARCTION (MI)
Operative placement of a permanent occlusion around the left anterior descending
artery of the rodent heart. Includes recording animal weight and one
echocardiography study one week post-occlusion.
3.
MYOCARDIAL ISCHEMIA/REPERFUSION
Operative placement of an occluder around the left anterior descending artery that is
removed after 30 or 60 minutes of occlusion. Includes recording rodent weight and one
echocardiography study after surgery (timeline to be discussed).
4.
GOLDBLATT I AND II MODELS OF HYPERTENSION
Goldblatt I - Operative placement of a clip on the left renal artery, as well as a right
nephrectomy.
CAF-EMIF
Rue du Bugnon 27
1005 Lausanne
Switzerland
+41 (0)21 314 6886
+41 (0)21 314 7977
www.unil.ch/caf/emif
caf.emif@chuv.ch
Goldblatt II - Operative placement of a clip on the left renal artery without right
nephrectomy.
5.
CAROTID BALLOON INJURY (rat only)
Operative injury of the common carotid artery endothelium by inflation of an
embolectomy catheter.
For each procedure, if additional echocardiographic studies are desired, please refer to the
“Request for Echocardiography” form.
Other Services
6.
ORGAN HARVEST
Includes body weight: heart weight ratio, slicing tissue, and preservation in formalin or
liquid nitrogen. Blood can also be retrieved and stored.
CHARGES
If you are interested in receiving an evaluation order, contact us at caf.emif@chuv.ch
Terms
1.
2.
3.
4.
Legal approval from the cantonal veterinary office must be obtained by the investigator
before starting experiments.
Investigator is responsible for transferring animals to the CAF housing facility, Rue du
Bugnon 27, and for retrieving animals or preserved tissue samples at the end of the
study.
Housing costs will be charged to the investigator.
Service will be charged according to service actually provided.
I agree to follow the terms and conditions described in this application.
I understand the terms and fees for the requested services.
P.I. Signature
Date
Billing contact person
Phone number
CAF-EMIF
Rue du Bugnon 27
1005 Lausanne
Switzerland
+41 (0)21 314 6886
+41 (0)21 314 7977
www.unil.ch/caf/emif
caf.emif@chuv.ch
Guidelines and Policies
Application for Services
The laboratory requesting service of the CAF agrees to provide the required animals for the planned
experiments, and to pay the CAF the appropriate fees. The applicant also acknowledges and accepts that
the final price and total number of animals required to complete the test may vary according to difficulties
encountered specifically with some rodent models.
Data and Data Ownership
All data will be processed, presented in an appropriate way and sent as an electronic document to the
applicant. The CAF personnel will be available to discuss various aspects of the test, including experimental
details and interpretation of the results.
All data generated upon request in the CAF strictly belong to the applicant investigator and his institution.
Thus, this data cannot be used by the CAF personnel for personal or institutional research purposes unless a
formal, written agreement of collaboration is established between CAF personnel and the investigator.
Pricing policy
Price information is available for each test on the Request for services forms. A written estimate of the fees
must be signed by the applicant before animals can be shipped to the Center.
Acknowledgements & Authorship
Investigators are expected to acknowledge the use of the CAF in their publications. In addition, whenever
time-consuming, labor-intensive experiments that require substantial scientific input are performed, the
contribution of the CAF staff should be recognized as a scientific collaboration, and involved CAF
collaborators will be authors in resulting publications