BIO101 Metabolism worksheet
Name ______________________________
1) The graph depicts the relationship between the potential energy of an enzyme-catalyzed
reaction and reaction progress:
a) Draw the path that the reaction would follow in the presence of a catalyst.
b) Label the transition state, TS of both the catalyzed reaction and the uncatalyzed reaction.
c) Label the activation energy, EA of both the catalyzed reaction and the uncatalyzed
reaction.
2) Consider the enzyme pathway. An increase in [E] leads to feedback inhibition of enzyme 3.
Which of the following will be affected? [ ] is the symbol for concentration. May choose
more that one answer
a)
b)
c)
d)
e)
[E]
[B]
[C]
[D]
[X]
3) A young child is rushed to the emergency room after ingesting ethylene glycol. Although it
is not itself harmful, ethylene glycol is quickly oxidized by alcohol dehydrogenase and then
undergoes further oxidation reactions to form a lethally toxic compound. Doctors respond by
administering high doses of ethanol, which inhibits the oxidation of ethylene glycol. Which of
the following mechanisms best explains ethanol's role in the treatment
a)
b)
c)
d)
Ethanol competes with ethylene glycol for the active site of the enzyme.
Ethanol competes with alcohol dehydrogenase for the enzyme.
Ethanol detoxifies the lethal compound.
Ethanol oxidizes the lethally toxic compound back into ethylene glycol.
BIO101 Metabolism worksheet
Put each the following terms in the appropriate column to indicate that they are related





Exergonic/endergonic
Spontaneous/non-spontaneous
Uphill reaction/downhill reaction
More stable reactants/less stable reactants
Reactants have less potential energy/reactants have more potential
energy



-Δ G/ + Δ G
Input of energy/release of energy
Anabolic/Catabolic
Column 1
Column2
Match the Letter of the name in left column to the definitions in the right column.
A. Activation Energy
____
Speeds up a chemical reaction and is unchanged in reaction.
B. Active Site of Enzyme
____
Shape of substrate matches shape of this region of an enzyme.
C. Competitive Inhibitor
____
Binds to enzyme but NOT at the active site and inhibits function
D. Enzyme
____
A reaction that needs energy input before it can proceed.
E. 1st Law of Thermodynamics ____
The energy needed to reach transition state
F. Allosteric regulator
A reaction that releases energy
____
nd
G. 2 Law of Thermodynamics ____ Energy cannot be created or destroyed but can be converted
H. Exergonic Reaction
___
Energy converted from 1 form to another, some wasted as heat.
I. Endergonic Reaction
____ Binds to active site of enzyme to block substrate