CHEM 1030L — Paper Chromatography of Amino Acids
Name:
______________
Date: ____________
Grade:
______________ (For Instructor use)
Laboratory Report
Section: ______________
Lab Sta. #: ________
PURPOSE
Instructions: Using 2-4 complete sentences written in third person with proper grammar and punctuation, briefly summarize the purpose of
this laboratory exercise. Include the learning objectives and the major outcomes.
Delete this text and write the purpose of this Lab Exercise here…
DATA AND OBSERVATIONS
*Hint: ALWAYS include the correct number of sig. figs.
Chromatogram Data Table
Instructions: For each spot (notice that the NutraSweet® sample has two spots!) 2-4 complete sentences written in third person
with proper grammar and punctuation, briefly summarize the purpose of this laboratory exercise. Include the learning objectives
and the major outcomes.
Sample
ID
Nut1
Nut2
Name
Spot
Height
(cm)
Solvent
Height
(cm)
Calculated
Rf *
Observations
Include spot color(s) and other observations
NutraSweet spot
#1
NutraSweet spot
#2
Tape the paper chromatogram to a plain sheet of paper (or below, if there is
enough room). Label the page and attach it here as part of the Data section.
Alternatively, take a photo of the chromatogram and insert the jpg file here.
CHEM 1030L — Paper Chromatography of Amino Acids
Laboratory Report
QUESTIONS AND ANALYSIS
INSTRUCTIONS: Using one or more complete sentences written in third person with proper grammar and punctuation, briefly respond to the
following questions.
Hint: Remember that points are awarded for each aspect of the question.
1.
Use Table 16.2 from the textbook to complete the following table.
a.
b.
c.
Type all of the information, except for the structure.
When all other sections are complete, print the report.
Draw by hand the amino acid structures before submitting the report.
Amino Acid
Name
3-Letter
Symbol
Polarity
Structure
(Nonpolar, polar,
acidic, or basic)
Drawn from textbook Table 16.2, p. 554
Lys
Asp
Lys
Asp
Draw the structure here ---->
Glu
Ala
Glu
Ala
Draw the structure here ---->
Pro
Phe
Pro
Phe
Draw the structure here ---->
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CHEM 1030L — Paper Chromatography of Amino Acids
Laboratory Report
2.
List the order of known amino acids in order of travel up the chromatogram. The amino acid with greatest amount
of travel (highest spot) should be at the top of the list.
Delete this text and type the response here...
3.
Explain the trend that exists between the order that the amino acids traveled up the chromatogram and their
relative polarities.
Delete this text and type the response here...
4.
Based the responses to questions 1 & 3, which amino acid would be predicted to travel highest on the
chromatogram: valine or histidine? Use information in Table 16.2. Explain the reasoning behind this answer.
Delete this text and type the response here...
5.
In the table below, type the name of each amino acid that corresponds to the designated spot on the
chromatogram.
Spot ID
Amino acid
1st NutraSweet®
1st NutraSweet®
Unknown # ______
CONCLUSION
INSTRUCTIONS: Write an essay, using complete sentences written in third person with proper grammar and punctuation, to briefly
summarize (1) the purpose of this laboratory exercise, (2) the work that was performed, (3) any problems encountered and how they could
affect the results, (4) explain how you identified the unknown sample and the two spots in the NutraSweet sample by (5) comparing
the appearance of each spot and the Rf for each spot with those of the known reference spots.
Use paragraphs to separate major ideas, however, do not number the paragraphs.
Delete this text and type the response here...
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