Supplementary Legends Supplementary Movie 1. Time lapse imaging of neural crest migration with Sox10E2 enhancer. Supplementary Figure 1. Additional loss of function studies. (A-D) COL9A3 and LTK morpholinated embryos. (E-F) Knockdown of neural crest and neural plate border specifiers causes upregulation of EBF1. Since ETS1 and MYB are thought to act as activators such effects are most likely to be indirect. Supplementary Table 1. Cranial Neural Crest Transcriptome Dataset. Enriched genes in migratory cranial neural crest cells. Supplementary Table 2. Genes depleted in the cranial neural crest in relation to whole embryo. Supplementary Table 3. Cranial neural crest transcriptome dataset genes validated by in situ hybridization. Supplementary Table 4. Transcriptional regulators identified of the migrating cranial neural crest dataset. 50 most-enriched transcriptional regulators unveiled by transcriptome analysis of the migrating CNC. Supplementary Table 5. Transcript levels of replicate libraries. Contains the transcript levels of the 200 most-enriched transcripts in each of the replicate libraries.