illumina DNA Sample Submission Form
DNA Sample Input Recommendations (Input DNA Quantity & Quality):
The ultimate success or failure of a library preparation strongly depends on using an accurately
quantified amount of input DNA. Follow these gDNA input recommendations:
1.


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Correct quantification of genomic DNA is essential.
We recommend an optimal quantity of 3-5 μg input DNA (minimal amount 2 μg).
We recommend using fluorometric based methods for quantification to provide accurate
quantification for dsDNA.
Please feel free to contact muriel.fragniere@dkf.unibe.ch or michele.ackermann@dkf.unibe.ch
if you want to use our Qubit 2.0 system.

Genomic DNA samples should be carefully collected to ensure that they are high molecular
weight and free of contaminants (RNA or small nucleic acid fragments such as nucleotides).
Gel electrophoresis is a powerful means for revealing the condition (including the presence
or absence) of DNA in a sample. Impurities, such as detergents or proteins, can be revealed
by smearing of DNA bands. RNA, which interferes with 260 nm readings, is often visible at the
bottom of a gel. A ladder or smear below a band of interest may indicate nicking or other
damage to DNA.
Please provide a digital image of a gel for each sample with clearly indicated sample
identity and size standard (give band sizes).
2.
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3.
Please fill in the form and submit it with your samples
Sample Name
Organism
Conc.(ng/μl)
Qubit
Volume
(μl)
Sequencing*
1
2
3
4
5
6
7
8
9
10
* standard options:
4.
(single-end 100 bp)
(paired-end 2 x 100 bp)
(single-end 250 bp, “rapid mode”, costs are higher per base)
(paired-end 2 x 250 bp, “rapid mode”, costs are higher per base)
Please enter the sample- or library request into the LIMS after discussing the project:
http://vetsrv03.campus.unibe.ch/lims
Do not hesitate to contact muriel.fragniere@dkf.unibe.ch or michele.ackermann@dkf.unibe.ch
if there are any questions.
Your contact details:
Contact person:
Institution:
E-mail address:
Phone:
SE100
PE100
SE250
PE250