illumina RNA Sample Submission Form
RNA Sample Input Recommendations (Input RNA Quantity & Quality) for whole transcriptome
sequencing:
The ultimate success or failure of a library preparation strongly depends on using an accurately
quantified amount of input RNA
1.

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Correct quantification of total RNA is essential.
We recommend to include a DNAse step with the RNA isolation method.
We recommend an optimal quantity of 1-4 μg input total RNA
We recommend using fluorometric based methods for quantification to provide accurate
quantification for RNA.
Please feel free to contact muriel.fragniere@dkf.unibe.ch or michele.ackermann@dkf.unibe.ch
if you want to use our Qubit 2.0 system.

It is very important to use high-quality RNA as starting material. Use of degraded RNA can
result e.g. in low yield, or over-representation of the 3’-ends of the RNA molecules.
We recommend that you check total RNA integrity following isolation using an Agilent 2100
Bioanalyzer with RNA Integrity Number (RIN) value greater than or equal to 8.
Please provide a pdf report showing the Bioanalyzer trace.
Please feel free to contact muriel.fragniere@dkf.unibe.ch or michele.ackermann@dkf.unibe.ch
if you want to use our Agilent 2100 Bioanalyzer system.
2.
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3.
Please fill in the form and submit it with your samples
Sample Name
Organism
Conc. (ng/μl)
Qubit
Volume
(μl)
Sequencing*
1
2
3
4
5
6
7
8
9
10
* standard options:
4.
(single-end 100 bp)
(paired-end 2 x 100 bp)
(single-end 250 bp, “rapid mode”, costs are higher per base)
(paired-end 2 x 250 bp, “rapid mode”, costs are higher per base)
Please enter the sample- or library request into the LIMS after discussing the project:
http://vetsrv03.campus.unibe.ch/lims
Do not hesitate to contact muriel.fragniere@dkf.unibe.ch or michele.ackermann@dkf.unibe.ch
if there are any questions.
Your contact details:
Contact person:
Institution:
E-mail address:
Phone:
SE100
PE100
SE250
PE250