SUPPLEMENTARY INFORMATION
Depth (cmbsf)
0
0
10
20
30
Mn2+ ( M)
Fe2+ ( M)
Sulphate (mM)
40 0
20 40 60 80 100 120 140 0
20
40
60
80 100 120
100
363
365
371
383
387
389
391
200
300
400
500
Supplementary Figure S1. Depth profiles of pore-water geochemistry constituents
5
(sulphate, manganese and iron) of Baffin Bay sites. All sites except 365, 383 and 387
were described in detail elsewhere (Algora et al., 2013).
10
Supplementary Figure S2. Depth profiles of organic carbon content in the sediments
measured as Total Organic Carbon (TOC) in weight % of all cores under
investigation.
1
Core 363
Core 365
Core 371
15
Core 383
Core 387
Core 389
2
Core 391
Supplementary Figure S3. Photographs of the cores described in this study.
3
0 50
150
OTUs
150
0 50
OTUs
250
Site 365
250
Site 363
0
500
1000 1500 2000 2500
0
500
# Sampled seqs
250
Site 383
0 50
150
OTUs
250
150
0 50
OTUs
# Sampled seqs
Site 371
0
500
1000 1500 2000 2500
0
500
# Sampled seqs
250
0 50
150
OTUs
150
OTUs
250
Site 389
0 50
0
500
1000 1500 2000 2500
0
500
# Sampled seqs
(cmbsf)
25
75
125
175
225
275
325
375
415
150
250
Site 391
0
500
.
1000 1500 2000 2500
# Sampled seqs
0 50
OTUs
1000 1500 2000 2500
# Sampled seqs
Site 387
20
1000 1500 2000 2500
1000 1500 2000 2500
# Sampled seqs
Supplementary Figure S4. Rarefaction curves for 0.03 OTUs for bacterial retrieved
sequences. Data from the corresponding sampled depths are plotted per sediment site.
4
20 40 60 80 100
Site 365
0
OTUs
0
OTUs
20 40 60 80 100
Site 363
0
100
200
300
400
500
600
0
100
100
200
300
400
500
20 40 60 80 100
600
0
600
100
300
400
500
600
500
600
Site 389
0
OTUs
Site 387
20 40 60 80 100
20 40 60 80 100
200
# Sampled seqs
0
OTUs
500
Site 383
# Sampled seqs
0
100
200
300
400
500
600
0
100
20 40 60 80 100
# Sampled seqs
Site 391
0
100
200
300
300
400
(cmbsf)
25
75
125
175
225
275
325
375
415
400
# Sampled seqs
200
# Sampled seqs
0
OTUs
400
0
OTUs
Site 371
0
25
300
# Sampled seqs
0
OTUs
20 40 60 80 100
# Sampled seqs
200
500
600
.
Supplementary Figure S5. Rarefaction curves for 0.03 OTUs for Archaea. Several
depths are plotted per site in each graph.
5
Supplementary Figure S6. Archaeal and bacterial OTU profiles with depth. The
30
number of sampled bacterial and archaeal sequences for the OTU analysis was 2828
and 636, respectively
6
35
Supplementary Figure S7. Correlation analysis of observed richness (Sobs, top row) and inverted Simpson diversity index (bottom row) and for
bacterial sequences with the environmental parameters sediment depth, TOC content, sulphate, Fe2+, and Mn2+ concentrations. Correlations with
P < 0.05 were considered significant. Continuous lines represent a linear model (formula provided above each panel) for the scatterplots and dotdashed lines show local trends by locally weighted scatterplot smoothing (LOWESS) using a smoothing factor of 2/3 (Cleveland 1979)
7
40
Supplementary Figure S8. Differential abundance of taxa between the basin (383,
387, 389, 391) and shelf (363, 365, 371) samples according to the t-test based
metastats analysis. Differentially abundant taxa having statistically significant
45
differences (P≤0.05, after the false discovery rate estimation for multiple hypothesis
testing) are indicated with asterisks (*), while error bars represent the calculated
standard errors.
8
Supplementary Figure S9. Mantel test based correlation of changes in microbial community structure (expressed as the Bray-Curtis
50
dissimilarity) with changes in the environmental variables of Mn2+ concentration, TOC content and sulphate concentration for Bacteria (upper
row) and Archaea (lower row). Continuous lines represent a linear model (formula provided above each panel together with the Mantel
correlation score r) for the scatterplots and dot-dashed lines show local trends by locally weighted scatterplot smoothing (LOWESS) using a
smoothing factor of 2/3 (Cleveland 1979).
9