Table S1 Information for all lakes involved in the experiment. Water chemistry data was
collected between May 2014 to August 2014 (S.E. Arnott, unpublished data) and averaged over
this period. Lake Opinicon was the source of all mesocosm water, while zooplankton were
collected from the other four lakes.
Lake
Lindsay
Long
Opinicon
Round
Warner
Latitude (N)
Area
Longitude (W)
(ha)
44°32'14.2"
-76°23'25.1"
44°31'40.5"
-76°24'10.0"
44°33'35.4"
-76°19'41.0"
44°32'16.9"
-76°24'00.3"
44°31'41.4"
-76°22'53.0"
DOC
Cond.
TP
Max Depth (m)
pH
31.5
10.9
8.11
6.02
249.60
10.45
15.5
26.0
8.22
4.04
180.72
10.15
788.0
11.3
8.08
4.74
187.78
18.51
15.0
30.1
8.08
3.54
152.22
8.16
9.2
6.4
8.07
5.32
266.80
12.81
(mg L-1) (μS cm-1) (μg L-1)
Table S2 Minimum adequate model (MAM) structure, statistical methodology, descriptive
statistics, and R2 values (calculated using the pseudo-R2 methods from Zuur et al. 2009) for
abundance (through time) and presence/absence (4th week following 2-2.5 average growing
weeks) models. ‘Size’, ‘Number’, and ‘Time’ entries under the ‘MAM’ heading represent the
predictors that were retained in the MAMs.
Metric
Abundance –
all weeks
P/A – final
week
MAM
Descriptive
Statistics
Mean: 2.4
Size*Number*Time
Number
Variance:
22.13
Minimum
success: 0
Minimum
failure: 3
Distribution
R2
Zero inflated
Negative
Binomial
0.87
Zero inflated
Binomial
0.77
Table S3 Abundance through time model coefficients. The propagule number category for the
single addition treatments (Number – 1) is used as the base level of ‘Number’ in the model.
Term
Estimate
SE
Z-value
P
Intercept
0.9043
0.4459
2.03
0.04256
Size
0.0989
0.0262
3.78
0.00016
Time
-0.5179
0.2086
-2.48
0.01302
Number – 2
0.6731
0.7507
0.90
0.36986
Number – 4
-0.5378
1.1429
-0.47
0.63795
Size:Time
0.0138
0.0117
1.18
0.23856
Size:Number – 2
-0.0472
0.0440
-1.07
0.28308
Size: Number – 4
0.0497
0.0685
0.73
0.46840
Time:Number – 2
-0.3273
0.3508
-0.93
0.35083
Time:Number – 4
0.3331
0.6062
0.55
0.58262
Size:Time:Number – 2
0.0136
0.0200
0.68
0.49603
Size:Time:Number – 4
-0.0868
0.0384
-2.26
0.02398
Fig. S1 Daytime average temperature (solid line) and overall average (dashed line) for the QUBS area
over the period in which the experiment was conducted. Note that the field site was shaded, and cover
was provided to all tanks, which would lead to cooler temperatures than those shown and reduce daily
fluctuations.
Fig. S2 Temperature variability (July 4th – August 31st) at QUBS within each day (measured every 15
minutes) and within each week (8 total weeks) for the period over which the experiment was conducted.