SUPPLEMENTARY INFORMATION
Supplementary Data Table 1
Ultra-structural fibre features of five fibres from the dark brown 0 hour UV,
baseline copper sample, detailing observations of damage to the cell membrane complex (CMC) in the cortical
and cuticle regions, to the cytoplasmic remnant in the cortical region and surrounding melanine granules, and to
the cuticle layers.
Parameter
Cuticle layers
Dark brown 0 hour UV, baseline Cu
Hair Number
1
2
3
4
3-6
4-6
3-5
4-8
5
7-8
% Endocuticle holes
% Cuticle CMC normal
% Cuticle CMC separated
% Cuticle CMC diffuse
15
20
<5
75
<5
30
<5
65
<5
25
<5
70
<5
45
<5
50
<5
70
<5
25
Cortical holes
Medium
Few
Medium
Few
Few
Cytoplasmic remnant damage
Slight
Slight
Medium
Slight
Slight
Melanin grain damage
Slight
Slight
Slight
Slight
Medium
% Normal cortical CMC
95
85
95
90
95
% Indistinct, separated or damaged
cortical CMC
<5
15
5
10
5
Supplementary Data Table 2
Ultra-structural fibre features of five fibres from the light brown 0 hour UV,
baseline copper sample, detailing observations of damage to the cell membrane complex (CMC) in the cortical
and cuticle regions, to the cytoplasmic remnant in the cortical region and surrounding melanine granules, and to
the cuticle layers.
Parameter
Cuticle layers
Light brown 0 hour UV, baseline Cu
Hair No.
1
2
3
3-6
6-7
4-6
4
5-8
5
3-6
% Endocuticle holes
% Cuticle CMC normal
% Cuticle CMC separated
% Cuticle CMC diffuse
5
90
<5
<5
<5
50
<5
45
20
90
<5
<5
<5
40
<5
55
40
70
5
25
Cortical holes
Few
Few to
medium
Medium
Few to
medium
Medium
Cytoplasmic remnant damage
Slight
Slight
Medium
Slight
Severe
Melanin grain damage
Slight
Slight
Slight to
medium
Slight
Medium
% Normal cortical CMC
95
95
80
80
90
% Indistinct, separated or damaged
cortical CMC
<5
<5
20
20
10
Supplementary Data Table 3
Ultra-structural fibre features of five fibres from the dark brown 40 hour
UV, baseline copper sample, detailing observations of damage to the cell membrane complex (CMC) in the
cortical and cuticle regions, to the cytoplasmic remnant in the cortical region and surrounding melanine
granules, and to the cuticle layers.
Dark brown 40 hour UV, baseline Cu
Hair Number
1
2
3
4
5-8
4-8
3-7
6-7
Parameter
Cuticle layers
5
0-4
% Endocuticle holes
% Cuticle CMC normal
% Cuticle CMC separated
% Cuticle CMC diffuse
<5
80
<5
15
<5
90
<5
<5
15
90
<5
<5
15
80
<5
15
50
n/a
n/a
n/a
Cortical holes
Medium
Medium
to severe
Medium
Medium
Medium
to severe
Cytoplasmic remnant damage
Slight
Slight
Slight
Severe
Medium
Severe
Melanin grain damage
Medium
Medium
Medium
Slight
Slight
% Normal cortical CMC
95
95
95
90
85
% Indistinct, separated or damaged
cortical CMC
<5
<5
<5
10
15
Supplementary Data Table 4
Ultra-structural fibre features of five fibres from the light brown 40 hour
UV, baseline copper sample, detailing observations of damage to the cell membrane complex (CMC) in the
cortical and cuticle regions, to the cytoplasmic remnant in the cortical region and surrounding melanine
granules, and to the cuticle layers.
Parameter
Cuticle layers
Light brown 40 hour UV, baseline Cu
Hair No.
1
2
3
4-6
5-6
4-6
4
5-8
5
4-8
% Endocuticle holes
% Cuticle CMC normal
% Cuticle CMC separated
% Cuticle CMC diffuse
5
75
<5
20
<5
70
15
15
5
15
<5
80
50
50
<5
45
<5
80
<5
15
Cortical holes
Medium
Few
Few to
medium
Medium
Medium
Cytoplasmic remnant damage
Medium
Slight
Slight
Slight to
medium
Severe
Melanin grain damage
Medium
Slight
Slight
Slight
Slight
% Normal cortical CMC
85
95
95
95
95
% Indistinct, separated or damaged
cortical CMC
15
<5
<5
<5
<5
Supplementary Data Table 5
Ultra-structural fibre features of five fibres from the dark brown 0 hour UV,
high copper sample, detailing observations of damage to the cell membrane complex (CMC) in the cortical and
cuticle regions, to the cytoplasmic remnant in the cortical region and surrounding melanine granules, and to the
cuticle layers.
Dark brown 0 hour UV, high Cu
Parameter
Cuticle layers
1
3-4
2
0-3
Hair No.
3
3-6
4
5-6
5
4-7
% Endocuticle holes
% Cuticle CMC normal
% Cuticle CMC separated
% Cuticle CMC diffuse
<5
70
<5
25
20
<5
20
85
<5
<5
<5
20
15
20
<5
75
50
50
<5
45
Cortical holes
Few to
medium
Medium
Few
Medium
Medium
Cytoplasmic remnant damage
Slight
Slight
Slight
Slight
Medium
Melanin grain damage
Slight
Slight
Slight
Slight
Medium
% Normal cortical CMC
80
80
75
70
85
% Indistinct, separated or damaged
cortical CMC
20
20
25
30
15
Supplementary Data Table 6
Ultra-structural fibre features of five fibres from the light brown 0 hour UV,
high copper sample, detailing observations of damage to the cell membrane complex (CMC) in the cortical and
cuticle regions, to the cytoplasmic remnant in the cortical region and surrounding melanine granules, and to the
cuticle layers.
Light brown 0 hour UV, high Cu
Parameter
Cuticle layers
1
4-7
2
4-7
Hair No.
3
6-8
% Endocuticle holes
% Cuticle CMC normal
% Cuticle CMC separated
% Cuticle CMC diffuse
<5
90
<5
5
40
55
<5
40
<5
50
<5
45
50
85
<5
10
25
90
5
5
Cortical holes
Few
Medium
Few
Medium
Medium
to severe
Cytoplasmic remnant damage
Slight
Severe
Slight
Medium
Severe
4
4-7
5
2-8
Melanin grain damage
Slight
Medium
Slight
Slight
Medium
% Normal cortical CMC
80
85
80
95
90
% Indistinct, separated or damaged
cortical CMC
20
15
20
5
10
Supplementary Data Table 7
Ultra-structural fibre features of five fibres from the dark brown 40 hour
UV, high copper sample, detailing observations of damage to the cell membrane complex (CMC) in the cortical
and cuticle regions, to the cytoplasmic remnant in the cortical region and surrounding melanine granules, and to
the cuticle layers.
Parameter
Cuticle layers
Dark brown 40 hour UV, high Cu
Hair No.
1
2
3
5-7
2-4
6-7
4
5-8
5
5-7
% Endocuticle holes
% Cuticle CMC normal
% Cuticle CMC separated
% Cuticle CMC diffuse
5
90
<5
5
5
80
10
10
15
85
15
10
10
90
15
5
20
85
<5
10
Cortical holes
Few
Medium
Medium
Medium
Many
Cytoplasmic remnant damage
Slight
Medium
Slight
Slight
Severe
Melanin grain damage
Slight
Slight
Medium
Slight
Medium
% Normal cortical CMC
95
95
90
90
85
% Indistinct, separated or damaged
cortical CMC
5
5
10
10
15
Supplementary Data Table 8
Ultra-structural fibre features of five fibres from the light brown 40 hour
UV, high copper sample, detailing observations of damage to the cell membrane complex (CMC) in the cortical
and cuticle regions, to the cytoplasmic remnant in the cortical region and surrounding melanine granules, and to
the cuticle layers.
Parameter
Cuticle layers
Light brown 40 hour UV, high Cu
Hair No.
1
2
3
3-4
6-8
4-7
4
3-6
5
5-10
% Endocuticle holes
% Cuticle CMC normal
% Cuticle CMC separated
% Cuticle CMC diffuse
10
55
20
25
<5
85
<5
10
60
50
10
40
30
80
10
10
20
35
<5
60
Cortical holes
Medium
Few to
medium
Many
Medium
to many
Medium
to many
Cytoplasmic remnant damage
Slight
Slight to
medium
Very
severe
Severe
Severe
Melanin grain damage
Slight
Slight
Medium
to severe
Severe
Severe
% Normal cortical CMC
90
95
75
95
70
% Indistinct, separated or damaged
cortical CMC
10
5
25
5
30
Supplementary Data Figure 1
Low magnification micrograph showing few cortical holes and little
endocuticle damage. This image is from a 0 hour UV, baseline Cu dose sample.
Supplementary Data Figure 2
Hair cortex showing normal (tri-layered) cortical cell membrane complex
(CMC) and melanin granules. This image is from a 0 hour UV, baseline Cu dose sample.
Supplementary Data Figure 3
Hair cortex showing some indistinct (white arrow, black border) and some
normal (black arrow, white border) cortical cell membrane complex (CMC). This image is from a 0 hour UV,
high Cu dose sample.
Supplementary Data Figure 4
Micrograph showing total cuticle loss and separated cortical cell membrane
complex (CMC). Most melanin granules are associated with holes in the cortex and there are some holes (white)
where melanin granules have been removed. This image is from a 40 hour UV, baseline Cu dose sample. Note:
a partial fold in the specimen (top left corner) is an artefact.
Supplementary Data Figure 5
Cuticle region showing normal cuticle cell membrane complex (CMC). This
image is from a 40 hour UV, baseline Cu dose sample.
Supplementary Data Figure 6
Micrograph showing cortex in relatively good condition. This image is from
a 40 hour UV, baseline Cu dose sample.
Supplementary Data Figure 7
Cuticle region showing endocuticle holes (white holes between cuticle
layers). This image is from a 40 hour UV, high Cu dose sample.
Supplementary Data Figure 8
Hair cortex showing severe damage to cytoplasmic remnants. This image is
also from a 40 hour UV, high Cu dose sample.
Supplementary Data Figure 9
Micrograph showing multiple cuticle layers and melanin granules from a
part of the hair that is relatively undamaged. This image is from a 40 hour UV, high Cu dose sample.
Supplementary Data Figure 10 Cuticle region showing diffuse cuticle cell membrane complex (CMC),
without the 3-layer differentiation visible in normal CMC. This image is from a 40 hour UV, baseline Cu dose
sample.
Supplementary Data Figure 11 Low magnification micrograph showing severe endocuticle damage. This
image is from a 40 hour UV, baseline Cu dose sample.
Supplementary Data Figure 12 Tandem mass spectra from 40 hour high copper-level B light brown hair,
peptide NQYEALVETNR from Keratin 33b, as extracted from Mascot.
Supplementary Data Table 9
sample peptides.
Sample
Modification
DB
0 Oxidation (Met)
hour
base b
DB
0 Quinone
hour
base b
DB
0 Hydroxyquinone
hour
base b,c
DB
0 Oxidation (Met)
hour low
b,c
DB
0 Oxidation (Met)
hour low
b,c
DB
0 Oxidation (Met)
hour low
b,c
DB
0 Oxidation (Pro)
hour low
b
DB
0 Oxidation (Lys)
hour low
c
DB
0 Trioxidation
hour low (Cys)
c
DB
0 Oxidation (His)
hour
high a
DB
0 Oxidation (Pro)
hour
high b,c
DB
0 Oxidation (Pro)
hour
high b
DB
0 Oxidation (Lys)
hour
high b
DB
0 Oxidation (Lys)
hour
high b
DB
0 Dioxidation
hour
(Cys)
high b
DB
0 Oxidation (Pro)
hour
high b
DB
0 Oxidation (Met)
hour
high c
DB
0 Oxidation (Pro)
hour
high c
DB
0 Oxidation (Pro)
hour
Source peptide and protein information for modifications observed in
Weight
1
Peptide
DLNMDCIIAEIK
Protein
Keratin 33b
Fibre Location
Intermediate
cortex [1,2]
2
PYNFCLPSLSCR
Keratin 33b
Intermediate
cortex [1,2]
filament,
3
NQYEALVETNR
Keratin 33b
Intermediate
cortex [1,2]
filament,
1
QDMACLIR
Keratin
86/83/81
Intermediate
cortex [1]
filament,
1
DLNMDCIVAEIK
Keratin 83
Intermediate
cortex [1]
filament,
1
DLNMDCIIAEIK
Keratin 81/86
Intermediate
cortex [1]
filament,
1
PPELQEQLK
Glutamine-rich
protein
1
KEDLRLMLIELK
Nesprin-2
Cytoskeletal
3
ARLECEINTYR
Keratin
31/33a/33b/34
Intermediate
cortex [1]
1
IEKMQELHAA
A-kinase anchor
protein
Cytoskeletal [3]
1
LNVEVDAAPAV
DLNQV
Keratin 33b
Intermediate
cortex [1,2]
1
GKWEPEPSSKPPR
Serine-threonine
protein kinase
Follicles [4]
1
GKWEPEPSSKPPR
Serine-threonine
protein kinase
Follicles [4]
1
GKWEPEPSSKPPR
Serine-threonine
protein kinase
Follicles [4]
2
WLCAVEGER
Kinesin-like
protein
Follicles [2, 5]
1
IAEIEPVER
Titin
Arrector pili
1
SDLEAQMESLKE
ELLSLK
Keratin 33b
Intermediate
cortex [1,2]
1
AGPGTEPDKILR
Dynaminbinding protein
1
GPGTEPDKILR
Dynaminbinding protein
filament,
filament,
filament,
filament,
high c
DB
0
hour
high c
DB
0
hour
high c
DB
0
hour
high c
DB 20
hour
base a
DB 20
hour
base a
DB 20
hour
base c
DB 20
hour
base c
DB 20
hour
base c
DB 20
hour low
a
DB 20
hour low
a
DB 20
hour low
a
DB 20
hour low
a
DB 20
hour low
a
DB 20
hour low
a
DB 20
hour low
a
DB 20
hour low
b
DB 20
hour low
b
DB 20
hour low
b
DB 20
hour low
c
Oxidation (Trp)
1
DVEAWFFSKTEELNK
Keratin 15
Follicle stem cells [6-8]
Oxidation (Phe)
1
DVEAWFFSKTEELNK
Keratin 15
Follicle stem cells [6-8]
Oxidation (Phe)
1
DVEAWFFSKTEELNK
Keratin 15
Follicle stem cells [6-8]
Trioxidation
(Cys)
3
Keratin 33a
Intermediate
cortex [1,2]
filament,
Oxidation (Cys)
1
Keratin 33a
Intermediate
cortex [1,2]
filament,
Trioxidation
(Cys)
3
SDLEAQVESLKEELLC
LK
NTLRCQLGDRLNVE
VD
AAPTVDLNQVLNET
R
ARLECEINTYR
Keratin 34
Intermediate
cortex [1]
filament,
Oxidation (Met)
1
QDMACLIR
Keratin 81
Intermediate
cortex [1]
filament,
Oxidation (Met)
1
DLNMDCIIAEIK
Keratin 81
Intermediate
cortex [1]
filament,
Oxidation (Cys)
1
Keratin 85
Intermediate
filament,
cortex and cuticle [1]
Keratin 85
Intermediate
filament,
cortex and cuticle [1]
RCCESNLEPLFSGYIE
TLRR
Oxidation (Cys)
1
RCCESNLEPLFSGYIE
TLR
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Lys)
1
Oxidation (Pro)
1
Trioxidation
(Cys)
3
Oxidation (Met)
1
Oxidation (Met)
1
Trioxidation
(Cys)
3
Oxidation (Arg)
1
Zinc
protein
finger
Hair follicle stem cell [9]
Zinc
protein
finger
Hair follicle stem cell [9]
Zinc
protein
finger
Hair follicle stem cell [9]
Zinc
protein
finger
Hair follicle stem cell [9]
APELPKPKKN
APELPKPKKN
APELPKPKKN
APELPKPKKN
KAP 4.12
Matrix [10]
Keratin 33b
Intermediate
cortex [1,2]
filament,
Keratin 81
Intermediate
cortex [1]
filament,
KAP 4.7
Matrix
Triple
functional
domain protein
Cytoskeletal [11]
CCLRPVCGR
ETMQFLNDR
DLNMDCIIAEIK
CCLRPVCGR
QRTNFGSSKFEFET
DB 20
hour low
c
DB 20
hour
high a
DB 20
hour
high a
DB 20
hour
high a
DB 20
hour
high a
DB 20
hour
high a
DB 20
hour
high a, b
DB 20
hour
high a, b
DB 20
hour
high a, b
DB 20
hour
high a, b
DB 20
hour
high a
DB 20
hour
high a
DB 20
hour
high b
DB 20
hour
high c
DB 20
hour
high c
DB 20
hour
high c
DB 20
hour
high c
DB 40
hour
base a
DB 40
hour
base b
DB 40
hour
Oxidation (Phe)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Lys)
1
Oxidation (Pro)
1
Oxidation (Lys)
1
Oxidation (Trp)
1
Oxidation (Phe)
1
Oxidation (Phe)
1
Oxidation (Pro)
1
Trioxidation
(Cys)
3
Trioxidation
(Cys)
3
Trioxidation
(Cys)
3
Oxidation (Arg)
1
Oxidation (Phe)
1
Oxidation (Met)
1
Oxidation (Met)
1
Trioxidation
(Cys)
3
Oxidation (Pro)
1
Oxidation (Met)
1
QRTNFGSSKFEFET
Triple
functional
domain protein
Zinc
finger
protein
Cytoskeletal [11]
Zinc
protein
finger
Hair follicle stem cell [9]
Zinc
protein
finger
Hair follicle stem cell [9]
Zinc
protein
finger
Hair follicle stem cell [9]
Hair follicle stem cell [9]
APELPKPKKN
APELPKPKKN
APELPKPKKN
APELPKPKKN
A-kinase anchor
protein 9
Cytoskeletal [3]
Keratin 15
Follicle stem cells [6-8]
Keratin 15
Follicle stem cells [6-8]
Keratin 15
Follicle stem cells [6-8]
Titin
Erector pili
Keratin 35
Intermediate
filament,
cortex and cuticle [1]
Keratin 35
Intermediate
filament,
cortex and cuticle [1]
Keratin 33b
Intermediate
cortex [1,2]
Triple
functional
domain protein
Triple
functional
domain protein
Keratin 83
Cytoskeletal [11]
Intermediate
cortex [1]
filament,
Keratin 81
Intermediate
cortex [1]
filament,
Keratin 31
Intermediate
cortex [1,2]
filament,
Intermediate
cortex [1]
filament,
AQEEKIKELQK
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
IAEIEPVER
SDLEAQVESLKEELLC
LK
ARLECEINTYR
filament,
NQYEALVETNR
QRTNFGSSKFEFET
QRTNFGSSKFEFET
Cytoskeletal [11]
DLNMDCIVAEIK
DLNMDCIIAEIK
ARLECEINTYR
LAPVSLPR
QDMACLIR
StAR
related
lipid
transfer
protein 9
Keratin 86
base b
DB 40
hour
base b, c
DB 40
hour
base b
DB 40
hour
base c
DB 40
hour
base c
DB 40
hour
base c
DB 40
hour low
a
DB 40
hour low
b
DB 40
hour low
b
DB 40
hour low
b
DB 40
hour low
b, c
DB 40
hour low
b
DB 40
hour low
b
DB 40
hour low
b
DB 40
hour low
b
DB 40
hour
high a
DB 40
hour
high a
DB 40
hour
high a
DB 40
hour
high a, b
DB 40
hour
high b
DB 40
Oxidation (Met)
1
Oxidation (Met)
1
Oxidation (Trp)
1
Oxidation (Phe)
1
Oxidation (Phe)
1
Dioxidation (Trp)
2
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Met)
1
Oxidation (Met)
1
Oxidation (Trp)
1
Oxidation (Phe)
1
Oxidation (Phe)
1
Hydroxyquinone
(Tyr)
3
Dioxidation
(Cys)
2
Oxidation (Pro)
1
Oxidation (Pro)
1
Trioxidation
(Cys)
3
Dioxidation
(Cys)
2
Oxidation (Pro)
1
Keratin 83
Intermediate
cortex [1]
filament,
Keratin 83
Intermediate
cortex [1]
filament,
Keratin 15
Cuticle
Keratin 15
Cuticle
Keratin 15
Cuticle
DLNMDCIVAEIK
DLNMDCIIAEIK
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
Multiple
protein
C2
GILDIEVWGK
Glutamine-rich
protein
PRARDEAGVPR
Glutamine-rich
protein
PRARDEAGVPR
Keratin 81
Intermediate
cortex [1]
filament,
Keratin 86
Intermediate
cortex [1]
filament,
Keratin 15
Cuticle
Keratin 15
Cuticle
Keratin 15
Cuticle
Keratin 33
Intermediate
cortex [1,2]
KAP 4.7
Matrix
A-kinase anchor
protein
Cytoskeletal [3]
A-kinase anchor
protein
Cytoskeletal [3]
Keratin 35
Intermediate
filament,
cortex and cuticle [1]
Kinesin-like
protein
Follicles [2, 5]
QDMACLIR
DLNMDCIIAEIK
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
filament,
NQYEALVETNR
CQTTCCR
FIRESEPEPDVR
FIRESEPEPDVR
SDLEAQVESLKEELLC
LK
WLCAVEGER
QGDGQGEVQPRG
Proline-rich
hour
high b
DB 40
hour
high b
DB 40
hour
high b
DB 40
hour
high b
DB 40
hour
high b
DB 40
hour
high b
DB 40
hour
high c
LB
0
hour
base b
LB
0
hour
base c
LB
0
hour low
a
LB
0
hour low
a
LB
0
hour low
a
LB
0
hour low
a
LB
0
hour low
a
LB
0
hour low
a
LB
0
hour low
a
LB
0
hour low
b
LB
0
hour low
b
LB
0
hour low
b
LB
0
hour low
c
Oxidation (Met)
transmembrane
protein
Keratin 86
1
Intermediate
cortex [1]
filament,
Keratin 86
Intermediate
cortex [1]
filament,
A-kinase anchor
protein
Cytoskeletal [3]
Keratin 33b
Intermediate
cortex [1,2]
filament,
Keratin 33a
Intermediate
cortex [1,2]
filament,
Keratin 83
Intermediate
cortex [1,2]
filament,
Keratin 33b
Intermediate
cortex [1,2]
filament,
Keratin 33b
Intermediate
cortex [1,2]
filament,
A-kinase anchor
protein
Cytoskeletal [3]
A-kinase anchor
protein
Cytoskeletal [3]
Keratin 15
Follicle stem cells [6-8]
Keratin 15
Follicle stem cells [6-8]
Keratin 15
Follicle stem cells [6-8]
QDMACLIR
Oxidation (Met)
1
Oxidation (His)
1
Oxidation (Pro)
1
DLNMDCIIAEIK
IEKMQELHAA
LNVEVDAAPAVDLN
QV
Trioxidation
(Cys)
3
ARLECEINTYR
Trioxidation
(Cys)
3
CKLAELEGALQK
Hydroxyquinone
(Tyr)
3
Trioxidation
(Cys)
3
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Trp)
1
NQYEALVETNR
SDLEAQVESLKEELLC
LK
FIRESEPEPDVR
FIRESEPEPDVR
DVEAWFFSKTEELNK
Oxidation (Phe)
1
Oxidation (Phe)
1
Oxidation (Pro)
1
Oxidation (Lys)
1
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
Glutamine-rich
protein 2
PPELQEQLK
Keratin 35
Intermediate
filament,
cortex and cuticle [1]
Keratin 83
Intermediate
cortex [1,2]
filament,
Keratin 81
Intermediate
cortex [1]
filament,
Keratin 33b
Intermediate
cortex [1,2]
filament,
Keratin 33a
Intermediate
cortex [1,2]
filament,
ILDDLTLCKSDLEAQV
ESLKEELLCLK
Oxidation (Met)
1
Oxidation (Met)
1
Oxidation (Pro)
1
QDMACLIR
DLNMDCIIAEIK
LNVEVDAAPAVDLN
QV
Trioxidation
(Cys)
3
ARLECEINTYR
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high a
LB
0
hour
high b
LB
0
hour
high b
LB
0
hour
high b
LB
0
hour
high c
LB 20
hour
base a
LB 20
hour
base a
LB 20
hour
base a
LB 20
hour
base a
LB 20
hour
base b
LB 20
hour
Trioxidation
(Cys)
1
Oxidation (Cys)
2
KAP 9.3
Matrix
KAP 9.3
Matrix
KAP 9.3
Matrix
KAP 9.3
Matrix
G-protein
interactor
Cell adhesion [12]
G-protein
interactor
Cell adhesion [12]
G-protein
interactor
Cell adhesion [12]
G-protein
interactor
Cell adhesion [12]
G-protein
interactor
Cell adhesion [12]
Keratin 35
Intermediate
filament,
cortex and cuticle [1]
Titin
Erector pili
Keratin 86
Intermediate
cortex [1]
KAP 4.7
Matrix
Keratin 33a
Intermediate
cortex [1,2]
ATP-binding
cassette
Follicle [13]
ATP-binding
cassette
Follicle [13]
Titin
Erector pili
Keratin 33b
Intermediate
cortex [1,2]
A-kinase anchor
protein
Cytoskeletal [3]
CCQPTCCGSSCGQSS
SCAPVYCR
CCQPTCCGSSCGQSS
SCAPVYCR
Trioxidation
(Cys)
3
Dioxidation
(Cys)
3
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Glu)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Lys)
1
CCQPTCCGSSCGQSS
SCAPVYCR
CCQPTCCGSSCGQSS
SCAPVYCR
SSPPPRLR
SSPPPRLR
PESGGPCGKALPER
PESGGPCGKALPER
PESGGPCGKALPER
ILDDLTLCKSDLEAQV
ESLKEELLCLK
Oxidation (Pro)
1
Oxidation (Met)
1
Trioxidation
(Cys)
3
Oxidation (Lys)
1
IAEIEPVER
filament,
DLNMDCIIAEIK
CCLRPVCGR
SDLEAQVESLKEELLC
LK
Oxidation (Lys)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Trioxidation
(Cys)
3
Oxidation (His)
1
Hydroxykynureni
ne (Trp)
3
filament,
KDVLPGAE
KDVLPGAE
IAEIEPVER
NQYEALVETNR
IEKMQELHAA
ALEAHWSTLRHWLS
DVK
Cross-linking
factor
filament,
base b
LB 20
hour
base b
LB 20
hour low
a
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
b
LB 20
hour low
c
LB 20
hour low
c
LB 20
hour
high a
LB 20
hour
high a
LB 20
hour
high a
LB 20
hour
high a
LB 20
hour
high a
LB 20
Oxidation (Met)
1
Trioxidation
(Cys)
3
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Hydroxyquinone
(Tyr)
3
Oxidation (Pro)
1
Oxidation (Pro)
1
MVGMQPPQAPR
CTRPICEPCR
Seizure
threshold
protein
Keratinassociated
protein
Serotonin
receptor
Matrix
Cell signalling [14]
PKEPGELAGKK
Serotonin
receptor
Cell signalling [14]
Serotonin
receptor
Cell signalling [14]
Serotonin
receptor
Cell signalling [14]
Keratin 33b
Intermediate
cortex [1,2]
Bromodomain
protein
Epithelial [15]
Bromodomain
protein
Epithelial [15]
PKEPGELAGKK
GPKEPGELAGKK
GPKEPGELAGKK
filament,
NQYEALVETNR
PPDPDSEGSTK
PPDPDSEGSTK
1
Keratin 7
ELMSVKLALDIEIATY
R
Oxidation (Met)
1
Epithelial [15]
Bromodomain
protein 1
Epithelial [15]
Keratin 86
Intermediate
cortex [1]
filament,
Keratin 86
Intermediate
cortex [1]
filament,
Kinesin-like
protein
Follicles [1,2]
Keratin 86
Intermediate
cortex [1]
Keratin 15
Follicle stem cells [6-8]
Keratin 15
Follicle stem cells [6-8]
Keratin 15
Follicle stem cells [6-8]
KAP 4.5
Matrix [16]
AKMVPEKVPR
Oxidation (Pro)
1
AKMVPEKVPR
Oxidation (Pro)
1
Oxidation (Met)
Oxidation (Met)
Bromodomain
protein 1
DLNMDCIIAEIK
1
QDMACLIR
Dioxidation
(Cys)
2
Oxidation (Cys)
1
WLCAVEGER
RLCEGIGAVNVCVSS
SR
Oxidation (Trp)
1
Oxidation (Phe)
1
Oxidation (Phe)
1
Trioxidation
3
filament,
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
DVEAWFFSKTEELNK
SCCRPCCCQTTCCR
hour
high a
LB 20
hour
high a
LB 20
hour
high a
LB 20
hour
high a
LB 20
hour
high b
LB 20
hour
high b
LB 20
hour
high b
LB 20
hour
high c
LB 40
hour
base a
LB 40
hour
base b
LB 40
hour
base b
LB 40
hour
base b
LB 40
hour
base c
LB 40
hour low
a
LB 40
hour low
a
LB 40
hour low
a
LB 40
hour low
a
LB 40
hour low
b
LB 40
hour low
b
LB 40
hour low
b
(Cys)
Trioxidation
(Cys)
3
KAP 4.5
Matrix [16]
Nuclear mitotic
apparatus
Follicles [17]
KAP 4.5
Matrix [17]
SCCRPCCCQTTCCR
Hydroxykynureni
ne (Trp)
3
Hydroxyquinone
(Tyr)
3
Oxidation (Pro)
1
Oxidation (Pro)
1
AWQEKFFQKEQALS
TLQ
QYEPIMETNR
Glutamine-rich
protein
PPELQEQLK
Keratin 33b
Intermediate
cortex [1,2]
filament,
Keratin 81
Intermediate
cortex [1]
filament,
Keratin 34
Intermediate
cortex [1]
filament,
Keratin 33a
Intermediate
cortex [1,2]
filament,
Intermediate
cortex [1]
filament,
Intermediate
cortex [1]
filament,
LNVEVDAAPAVDLN
QV
Oxidation (Met)
1
Trioxidation
(Cys)
3
Trioxidation
(Cys)
3
Oxidation (Pro)
1
Oxidation (Met)
1
DLNMDCIIAEIK
ARLECEINTYR
SDLEAQVESLKEELLC
LK
CMYA5
AESEEEPDSR
Keratin 86
DLNMDCIIAEIK
Trioxidation
(Cys)
3
Oxidation (Met)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Lys)
1
Keratin 35
SDLEAQVESLKEELLC
LK
Keratin 86
DLNMDCIIAEIK
Zinc
protein
finger
Follicle stem cells [9]
Zinc
protein
finger
Follicle stem cells [9]
Zinc
protein
finger
Follicle stem cells [9]
Zinc
protein
finger
Follicle stem cells [9]
APELPKPKKN
APELPKPKKN
APELPKPKKN
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
APELPKPKKN
Bromodomain
protein
Epithelial [15]
Bromodomain
protein
Epithelial [15]
Serotonin
receptor
Cell signalling [14]
PPDPDSEGSTK
PPDPDSEGSTK
PKEPGELAGKK
LB 40
hour low
b
LB 40
hour low
b
LB 40
hour low
b
LB 40
hour low
c
LB 40
hour low
c
LB 40
hour
high a
LB 40
hour
high a
LB 40
hour
high a
LB 40
hour
high a
LB 40
hour
high a
LB 40
hour
high a
LB 40
hour
high a
LB 40
hour
high a
LB 40
hour
high b
LB 40
hour
high c
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Trioxidation
(Cys)
3
Trioxidation
(Cys)
3
Dioxidation
(Cys)
2
Oxidation (Pro)
1
Oxidation (Lys)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Oxidation (Pro)
1
Hydroxykynureni
ne (Trp)
3
Hydroxyquinone
(Tyr)
3
Hydroxyquinone
(Tyr)
3
Trioxidation
(Cys)
3
Serotonin
receptor
Cell signalling [14]
Serotonin
receptor
Cell signalling [14]
Serotonin
receptor
Cell signalling [14]
Keratin 86
Intermediate
cortex [1]
Keratin 35
Intermediate
filament,
cortex and cuticle [1]
Keratin 31
Intermediate
cortex [1,2]
PKEPGELAGKK
GPKEPGELAGKK
GPKEPGELAGKK
filament,
CKLAELEGALQK
SDLEAQVESLKEELLC
LK
MYCCALRSCSVPTGP
ATTFCSFDK
filament,
Ribosyltransfera
se
NPTTLEARLR
Proteoglycan 4
Follicles [18]
Proteoglycan 4
Follicles [18]
Proteoglycan 4
Follicles [18]
Proteoglycan 4
Follicles [18]
Nuclear mitotic
apparatus
Follicles [18]
Keratin 85
Intermediate
filament,
cortex and cuticle [1]
Keratin 33b
Intermediate
cortex [1,2]
filament,
Keratin 31
Intermediate
cortex [1,2]
filament,
PKEPAPTTPK
PKEPAPTTPK
PKEPAPTTPK
PKEPAPTTPK
AWQEKFFQKEQALS
TLQ
WQFYQNQRCCESN
LEPLFSGYIETLR
NQYEALVETNR
SDLEAQVESLKEELLC
LK
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