Recombination
All of the following are applications of recombinant DNA technology EXCEPT:
A. production of human proteins in bacterial cells.
B. using bacteria to detect the presence of carcinogens.
C. altering plants to make them resistant to pests.
D. treatment of human genetic disorders.
Reverse transcriptase produces which of the following?
A. cDNA
B. tRNA
C. mRNA
D. codons
Synthetic antisense nucleic acid molecules may one day be used for
A. genetic fingerprinting.
B. the control of genetic diseases.
C. the separation of different DNA molecules.
D. the creation of restriction enzymes.
All of the following are true statements concerning restriction enzymes EXCEPT:
A. They cut DNA molecules at specific sequences.
B. They are found in all cells.
C. There are two basic types of restriction enzymes.
D. They are an important tool of recombinant DNA technology.
All of the following can serve as vectors EXCEPT:
A. primers.
B. plasmids.
C. transposons.
D. viruses.
All of the following can carry large genes EXCEPT:
A. plasmids.
B. transposons.
C. viruses.
D. All of these can carry large genes.
A gene library contains which of the following?
A. Mutant strains of a particular microorganism.
B. Clones that contain fragments of an organism's entire genome.
C. Clones that contain all the genes from a single chromosome.
D. Both B and C are correct.
E. A, B, and C are correct.
Why is PCR such a useful technique?
A. It can be used in a variety of applications, such as the identification of unknown pathogens.
B. A large number of copies of a DNA molecule can be produced in a short amount of time.
C. A large number of copies can be made of a single DNA molecule.
D. All of the above are correct.
Which of the following is a correct sequence of events in the PCR process?
A. Priming, Extension, Denaturation
B. Extension, Denaturation, Priming
C. Denaturation, Extension, Priming
D. Denaturation, Priming, Extension
The DNA polymerase from which of the following microbes is used in PCR?
A. Haemophilus influenzae
B. Bacillus anthracis
C. Thermus aquaticus
D. Deinococcus radiodurans
In electrophoresis, all of the following are criteria by which DNA molecules can be separated EXCEPT:
A. shape.
B. sequence.
C. electrical charge.
D. size.
All of the following are true statements concerning the gel electrophoresis of DNA molecules EXCEPT:
A. The sizes of DNA molecules can be determined by the position to which they migrate in a gel.
B. Smaller DNA molecules move faster and farther than larger ones.
C. Gels used for electrophoresis of DNA are made out of agarose.
D. DNA molecules move through the gel towards the negative electrode.
Which of the following is thought to contribute to the infectious nature of the yeast Candida albicans?
A. It has extra genes not found in other yeasts.
B. It does not require nitrogen for growth.
C. It can change its growth patterns.
D. Both A and B are correct.
E. Both A and C are correct.
Which of the following involves molecules of single stranded DNA bound to glass slides or silicon
chips?
A. electroporation
B. PCR
C. DNA microarrays
D. Southern blots
Which of the following methods of introducing DNA into cells can be used on intact tissues?
A. protoplast fusion
B. injection
C. electroporation
D. All of these can be used on intact tissues.
Fluorescent nucleotides are associated with which of the following?
A. electroporation
B. DNA fingerprinting
C. Southern blotting
D. nucleotide sequencing
An oral vaccine against which of the following is currently being tested?
A. HIV
B. Mannheimia haemolytica
C. the SARS virus
D. Plasmodium falciparum
The shelf life of certain genetically-engineered tomatoes has been lengthened due to the use of which
of the following technologies?
A. DNA fingerprinting
B. protoplast fusion
C. PCR
D. antisense RNA
Which of the following is associated with recombinant DNA applications of herbicide resistance?
A. BGH-producing bacteria
B. beta-carotene-containing rice
C. MacGregor tomatoes
D. "Roundup-ready" soybeans
Which of the following is an example of xenotransplantation?
A. Transferring bacterial genes into a plant
B. Implanting pig heart valves into a human heart.
C. Transferring human genes into bacterial cells.
D. All of the above are examples of xenotransplantation.