NIH Filariasis Research Reagent Resource Center (FR3)
Experimental Techniques in Filariasis Research
13-16 May 2013
University of Georgia, College of Veterinary Medicine
Class Schedule
Instructors:
Andy Moorhead, DVM, MS, PhD
Steven Williams, PhD
Shelly Michalski, MS, PhD
Mike Dzimianski, MS, DVM
Prasit Supakorndej, BS(Pharm), MS, PhD
Susan Haynes, BS
John McCall, MS, PhD
Caroline Decker, AB
Erica Burkman, MS
Leanne Alworth, DVM, MS
Leah Mann, MS
University of Georgia
Smith College
University of Wisconsin-Oshkosh
University of Georgia
University of Georgia
Smith College
University of Georgia
Smith College
University of Georgia
University of Georgia
University of Wisconsin-Oshkosh
Support Personnel:
LaDonna Allen
Molly Riggs, BS
Christopher Evans, MS
University of Georgia
University of Georgia
University of Georgia
Locations:
Lectures: Room 312 in Driftmier Engineering Building
Labs: Room G5 in the Wildlife Health Building
Bioinformatics : Room 203 in Veterinary Building
Breakroom: Room G2 in the Wildlife Health Building
Day
Time
Event
Location
Monday
8:30-8:45
Welcome and Introductions
236
8:45-9:30
Lecture (Moorhead): FR3 parasite resources available
236
9:30-10:00
Lab (Dzimianski/Supakorndej): Use blood from cat
with B. malayi microfilariae (mf) to set up
membrane-feeders and start infecting mosquitoes
Insectary
10:00-10:15
Break
10:15-11:00
Lab (Dzimianski/Supakorndej): Collect L3 from
mosquitoes, examine L3 under microscope, inject
L3 into jirds
G5
11:00-11:45
Lecture (Moorhead): Overview of nematode
biology and introduction to filarioid nematodes
312
11:45-12:15
Lecture (Williams): Introduction to molecular labs
312
12:15-1:30
Lunch
1:30-2:30
Lab (Dzimianski/Supakorndej): Necropsy B.
malayi infected jirds; Collect L4 and microfilariae
from peritoneal cavity and examine under microscopy
G5
2:30-3:30
Lab (Haynes/Decker): Set up PCR Reaction
G5
3:30-4:30
Lecture (Williams): PCR and Sequencing to Identify
Filarioid Nematodes
312
4:30-4:45
Break
4:45-5:45
Lab (Haynes/Decker): Load PCR Gel
G5
(Meet in G5)
Tuesday
8:30-9:30
Lab (Dzimianski/Supakorndej): Collect adult
worms and microfilariae from peritoneal cavity
and examine under microscopy. Identify and
sort male and female worms.
G5
9:30-10:30
Lecture (Mann): A. viteae lecture
312
10:30-10:45
10:45-11:15
Break
Lab (Haynes/Decker): Set up DNA sequencing reactions
G5
11:15-12:15
Lecture (Moorhead): L3 Culturing
312
12:15-1:30
Lunch
1:30-2:00
Lab (Haynes): Techniques for washing & culturing
filarial worms
G5
2:00-2:30
Lab (Burkman): microfilariae isolation techniques
G5
2:30-5:45
Lab (Mann): Anti-Wolbachia surface protein
Immunohistochemistry.
G5
(Meet in 312)
Wednesday
8:30-9:30
Lecture (Alworth): Using gerbils in biomedical
research.
312
9:30-10:30
Lecture (Michalski): Transcriptome analysisApplications and Methods.
312
10:30-10:45
Break
10:45-11:15
Lab Demonstration (Dzimianski/Supakorndej):
Pick mosquito pupae; put mosquito eggs in water
for hatching into larvae.
Insectary
11:15-12:15
Lab (Haynes/Decker): Set up qRT-PCR
reactions.
G5
12:15-1:15
Lunch
1:15-2:15
Lab (Haynes): Worm Transfection techniques
G5
2:15-5:15
Lab (Michalski): Dissect freshly fed mosquitoes
and quantify midgut penetration of L1. Dissect day
8 and day 12 mosquitoes – L2 and L3 stages.
G5
5:15-5:45
Lab (Haynes): Worm Transfection slides
G5
.
(Meet in Insectary)
Thursday
8:30-9:00
Lab (Dzimianski/Supakorndej): Set up mosquito
larval cultures
Insectary
9:00-10:00
Lecture (Williams): Gene Expression Analysis
312
10:00-10:15
Break
10:15-12:15
Lecture (Lammie): Immunology and the Global
Effort to Eliminate Lymphatic Filariasis
12:15-1:30
Lunch
1:30-2:30
Lab (Dzimianski/Supakorndej): Necropsy of
Brugia sp. infected jirds with lymphatic infection
G5
2:30-3:30
Lecture (McCall): B. malayi / B. pahangi
vertebrate host(s) phase
312
3:30-3:45
Break
3:45-4:45
Lecture (McCall): D. immitis mosquito vector
phase, D. immitis vertebrate host(s) phase
312
4:45-5:45
Lecture (Williams): Bioinformatics and clone data
312
312
NIH Filariasis Research Reagent Resource Center (FR3)
Experimental Techniques in Filariasis Research
17-18 May 2013
University of Georgia, College of Veterinary Medicine
Class Schedule
Instructors:
Matt Rogers, PhD
Kevin Howe, PhD
Michael Paulini, PhD
John Martin, PhD
Chris Desjardins , PhD
University of Pittsburgh
WormBase
WormBase
Nematode.net
Broad Institute
Support Personnel:
LaDonna Allen
Molly Riggs, BS
Christopher Evans, MS
Erica Burkman, MS
University of Georgia
University of Georgia
University of Georgia
University of Georgia
Location: Room H203 in Veterinary Building
Day
Time
Event
Location
Friday
8:30-10:00
Lecture (Rogers): Basic B. malayi genome analysis
203
10:00-10:30
Break
10:30-12:30
Lecture (Rogers): Basic B. malayi genome analysis
12:30-1:30
Lunch
1:30-5:30
Lecture (Howe & Paulini): Mechanics of Wormbase
203
8:30-10:30
Lecture (Martin): Mechanics of Nematode.net
203
10:00-10:30
Break
10:30-1:00
Lecture (Desjardins): Use of Broad Institute filarial
genome data and software
203
1:00-1:15
Closing remarks and evaluations
203
Saturday
203