Lmon Arab Biol Cairo
ISSN 1110-5372
Http :w~vwarabiolo~istsor~
I4~ International Conference
Erna~l ~
15-19 April, 2007
Vol (27A) Zoology225—244 ,April,2007Faculty of Education of Suez,
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Rec. 15/3/2007
Suez Canal University, Egypt
APPLICABILITY OF BIOSORPTION TECHNOLOGY
REMEDIATE METAL-CONTAINING TANNING PROCESS
INDUSTRIAL EFFLUENT
*
**
TO
El-Fadaly, H.; El-Gammal, M.I.S.; El-Sayed
and Dma Makia***
Dept. of Microbiology, Fac. of Agric., Mansoura Univ., Mansoura, Egypt.
Dept. of Environmental Sciences, Fac. of Sci. of Damietta, Mansoura Univ..
Damietta, Egypt.
Dept. Microbiology. Soil, Water & Environment Res. Institute, ARC, Cairo,
Egypt.
Dept. of Environmental Res., Soil, Water & Environment Res. Institute,
ARC, Cairo, Egypt.
Key Words : Biosorption mineral bacterial chromium microorganisms.
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ABSTRACT
Applicability of biosorption technology to remediate metal-containing
tanning process (mineral department) industrial effluent was carried out. The
microbial treatment was conducted using two bacterial and two yeast strains.
These microbes namely Bacillus megaterlurn, 6SB, Serratia niarcescens, 3STA
and Saccharomyces cerevisiae, SC64 and Saccharomyces cerevisiae, SC66.
Biosorption of chromium by immobilized cells of S.ccrevisiae, SC64 and
immobilized cells of 13. Inegateriurn, 6SB, finally by Biosorption of chromium in
continuous system using bioreactor:
Batch systems using shake flask: was used for biosorption of som~ trace
metal ions using free microbial cells. The observed results showed that B.
megaterium, 6SB and S. cerevisiae, SC66 were the most active strains for Cd
removal since they gave 100% removal. The best Cr~’~ removal was achieved by
S. cerevisiae, SC64 and B.megatcriuni, 6SB being 95.078 and 94.47 %,
respectively. At the same time, the results showed that S. inarcescens, 3STA and
S. cerevisiae, SC64 exhibited maximum effect upon Mn removal (100%). The
four examined microorganisms exhibited maximum effect upon Pb removal
(100%). Selenium removal showed a good result since its remOval by 13.
megaterium, 6SB reached 91 .978 S. cerevisiae, SC64 showed the highest
removal of Zn (43.906%). The results indicated that the major portion of the
chromium metal was directly removed at the first 24 hr. Colour removal
efficiencies were 47.162, 42.258, 46.129 and 45.484 % for
%.
225
226
B. megateriurn, 6SB, S. marcescens, 3STA, S. cerevisiae, SC64 and S.
cercvisiae, SC66 after 24 hr, respectively.
Batch systems using bioreactors was applied to examine the biosorption
of chromium by immobilized S.cerevisiae, SC64 and B. megaterium, 6SB. The
results showed that the chromium metal content of the tested effluent was highly
reduced to 75.83 % after 4 h. The percentage of colour removal reached to the
maximum value to be 78.06 after 114 hour. The results obtained showed clearly
that the TN was completely assimilated during the first 18 hours of incubation.
TOC gradually decreased where they were reduced from 352.79 to 20.70 mg/I,
after 54 hr. Highly successful removal of chromium metal was reached by using
immobilized Bacillus megaterlum, 6SB (98.11%). The maximum colour removal
(66.43 %) was reached at the end of fermentation time (135 h). The TN was highly
consumed (82.89 %) after 6 hr of fermentation period.
Biosorption of chromium in continuous systems using bioreactor was
also examined. The column (52 cm high and 5.2 cm internal diameter) was filled
with 0.69 g of living immobilized cells of B. megaterium, 6SB in the form of
calcium alginate beads and working volume was 600 ml of tanning effluent (95 %)
in continuous fermentation usmg a feed with TA effluent at a dilution rate (D) of
0.0 17 hf’ and the biomass level in the reactor started with of 0.69 gIl. The higher
efficiency in chromium and colour removal was reached at the first three hours
being 69.98 %, and 39.93 %, respectively. The biomass was reached to its
maximum value of 19 mg/l. after 24 hr. The chromium metal uptake value reached
38.69 mg/g biosorbent from TA industrial effluent, after the first three hours.
Key words: Metal bioremediation, tanning process industnal effluent,
Bacillus megaterium, SerratEa marcescens and Saccharomyces cerevisiae,
Biosorption of chromium, immobilized cells, batch and continuous system.
INTRODUCTION
The problem of water pollution
by toxic heavy metal contamination
resulting from humans technological
activities has for long presented a
challenge Biosorption can be a part of
the solution. Some types of biosorbents
such as seaweeds, molds, yeasts,
bacteria or crab shells are examples of
biomass tested for metal biosorption
with very encouraging
results. The uptake of heavy jnetals
by biomass can in some cases reach
up to 50% of the biomass dry weight.
New biosorbents can be manipulated
for better efficiency and multiple
re-use to increase their economic
attractiveness (Vi~irtt tinti V~iI~}~y,
~I~)).
Biosorptton
has
been
intensively studied in recent years as
an economical treatment for metal
recovery from dilute industrial
effluents. Biosorption
‫‪‬‬
‫‪Applicability of biosorption technology to‬‬
‫‪remediate metal-containing tanning process‬‬
‫‪industrial efflueut‬‬
‫مجلة إتحاد البيولوجين العرب القاهرة‪ ,‬الصفحات‬
‫‪2007/4/1 ,224-225‬‬
‫أ‪.‬د‪ .‬حسين عبدهللا محمد الفضالى‬
‫أ‪.‬د‪ .‬مى ابراهيم عبد العزيز الجمال‬
‫دينا عبد الحليم مكية ‪ -‬محطة مياه شرب‬
‫سمير على السيد ‪ -‬مركز البحوث الزراعية‬