Appendix A2 Genetic structure of E. globulus samples
The genetic structure among the sample of E. globulus collected from the discovery
population was investigated using the computer program STRUCTURE and a data set
derived from 18 microsatellite loci. Of these microsatellites, six (CRC2, CRC5, CRC7,
CRC8, CRC10 and CRC 11) were designed by Steane et al. (2001), two (EMBRA 10 and
EMBRA 11) by Brondani et al. (1998), four (EMBRA23, EMBRA30, EMBRA38 and
EMBRA63) by Brondani et al. (2002), two (EMBRA 210 and EMBRA 213) by Brondani et
al. (2006), one (Eg131) by Thamarus et al. (2002) and three (EMBRA362, EMBRA712 and
EMBRA747) by D. Grattapaglia (pers. comm.). Multiplex PCRs were carried out using a
PCR cycle comprising: 15 minutes at 95°C, 30 cycles of 30 seconds at 94°C, 90 seconds at
57°C, 1 minute at 72°C; followed by a final 10 minutes at 60°C. The PCR products were
separated on an ABI 377 DNA sequencer at the Australian Genomic Research Facility
(AGRF) in Adelaide and analysed using GeneMapper® software (Version 3.7). The SSR
loci were highly polymorphic and informative with on average 21.7 alleles per loci.
Figure 1. Graph of ΔK calculated from 18 microsatellites for E. globulus microsatellite
following the method of Evanno et al. (2005). Evanno et al. (2005) provides a method for
finding the most likely number of clusters of genetically homogeneous groups of individuals
(K). The secondary peak at K=4 was deemed the best solution as it made the most biological
sense.
Figure 2 Proportion of membership at K=4 for all sampled individuals of E. globulus from
the discovery population based on microsatellite data. Each individual is represented by a thin
vertical line that can be partitioned into up to four coloured segments representing the
individual’s estimated membership (Q) into the K genetic clusters. Individuals are grouped
by region (above the figure) and race (below the figure).
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