Ampli1™ WGA Kits
How much input material can be amplified using Ampli1™ WGA Kits?
Ampli1™ WGA Kits have been optimized for amplification of the total DNA content of a single
cell; however, the kit works equally well to amplify DNA from multiple cells.
Can DNA from fixed or preserved cells be amplified with Ampli1™ WGA
Kits?
Silicon Biosystems has validated that preservatives, such as that used in the Veridex CellSave tube
and in the CellSearch ® System, or fixatives, such as 1–2% PFA, have little effect on the efficiency
of amplification with Ampli1™ WGA Kits. Stronger fixatives may lead to lower yields.
For questions regarding compatibility of Ampli1™ WGA Kits with other fixatives, please contact
us.
How much material is generated from a single cell amplified with the
Ampli1™ WGA Kits?
Ampli1™ WGA amplification generally produces 2-4 micrograms DNA.
What is the size of the DNA fragments that are generated by Ampli1™
WGA Kits?
Amplified DNA fragments range in size from 0.2–2kb.
Is the genomic amplification linear?
Yes. The Ampli1™ WGA Kit uses single primer-mediated PCR to provide linear amplification of
fragmented genomic DNA.
What downstream analyses can be performed with Ampli1™ WGAamplified DNA?
Amplified DNA may be used in a variety of downstream applications, including:
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Next generation and whole genome sequencing
Single nucleotide polymorphism (SNP) and mutation detection
Microsatellite analysis
Comparative genomic hybridization
Copy number variation (CNV) analysis
The Ampli1™ WGA Kit is for Research Use Only. Not for use in diagnostic procedures.
Please contact us if you have questions about the compatibility of Ampli1™ WGA-amplified DNA
with your downstream application.