Biosensor Technology for Smart Diagnostic Applications
Min-Gon Kim
Bionanotechnology Research Center, KRIBB, Daejeon 305-806, Korea
TEL: +82-42-860-4445, E-mail: mgkim@kribb.re.kr
Abstract
Biosensor is an integrative system of bioreceptor, physicochemical sensor and nano-materials or
-devices. It enables to provide highly miniaturized device with specific detection of various
materials such as proteins, DNA, injurious materials, viruses, and pathogens. In this
presentation, a recent trend of biosensor technology will be overviewed, and the biosensors
developed by our team introduced. They include nanoparticle, SPR (surface plasmon resonance),
and lateral flow assay (LFA) biosensors. As an example, for signal amplification without an
additional operation step in a gold nanoparticle (AuNP)-LFA, a new and simple method
utilizing two AuNP-antibody conjugates was developed. The 1st conjugate was the AuNP
immobilized with anti-troponin I antibody and blocked with bovine serum albumin (BSA), and
the 2nd conjugate was the AuNP immobilized with anti-BSA antibody and blocked with human
serum albumin. Two conjugates were encapsulated in different pads, respectively. The size of
two conjugates was very critical in the detection sensitivitys of troponin I. When 10 nm for the
1st and 40 nm for the 2nd were used, the detection sensitivity was increased about 1000 fold than
a conventional LFA. We could detect as low as 0.01 ng/mL troponin I in 10 min.