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Supplementary Figure Legends
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Fig. S1 Rarefaction curves for the amplicon pyrosequencing profiles of bulk soil samples from
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Japanese barberry using genomic DNA that was either sequenced individually or pooled. Panels
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(a) and (b) present the number of observed phylotypes as a function of the total number of
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sequences for bacterial 16S rRNA fungal ITS assays, respectively. These data demonstrate
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similar OTU richness in the individual (B2.1, B2.2, B2.3) and pooled (B2) genomic DNA
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samples from the bulk soil.
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Fig. S2 The bacterial community profiles obtained with 16S rRNA amplicon pyrosequencing of
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the bulk soil genomic DNA samples when sequenced as a pooled sample (B2) versus
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individually (B2.1, B2.2, B2.3). A weighted UniFrac significance test indicates there is no
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significant difference between the bacterial communities observed in these samples (p > 0.05).
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Colored bars represent the proportion of each taxa in the total bacterial community of the sample.
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Fig. S3 The fungal community profiles obtained with ITS amplicon pyrosequencing of the bulk
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soil genomic DNA samples when sequenced as a pooled sample (B2) versus individually (B2.1,
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B2.2, B2.3). A weighted UniFrac significance test indicates there is no significant difference
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between the bacterial communities observed in these samples (p > 0.05). Colored bars represent
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the proportion of each taxa in the total bacterial community of the sample.
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Fig. S4 Rarefaction curves charting the number of observed phylotypes as a result of the total
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number of sequences obtained per sample. The bacterial (16S rRNA) and fungal (ITS)
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rarefaction curves are shown in (a) and (b), respectively. These data indicate the current depth of
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sequencing has not revealed the total microbial communities, although more of the fungal
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community members were detected than the bacterial community members (as determined by the
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slope of the curve).
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