Growing Plants Technical & Teaching Notes Related sheets Plant nutrients; Making and testing nutrients; Homemade fertilisers Introduction and context This guidance is for the three student sheets: Soil culture • Water culture • Floating culture Each of these sheets provides a protocol that may be used to investigate the growth of seedlings and describes an investigation that students could carry out. The protocols can be used in other investigations. The protocols could be used to help plan and carry related investigations for example in the sheets Making and testing nutrients and Homemade fertilisers. Safety Notes In the quantities used the substances used are low hazards. Equipment and materials Growing solutions Standard Sachs solutions are available, but a ‘complete-nutrient’ growing solution can be prepared by dissolving the following compounds in 1 dm 3 of deionised water. 0.70 g KNO3 0.25 g CaH4(PO4)2.2H2O 0.25 g CaSO4.2H2O 0.25 g MgSO4.7H2O 0.08 g NaCl Nutrient-deficient growing solutions can be prepared similarly, but with these changes: Nutrient solution deficient in Replace With Nitrogen KNO3 0.52 g KCl Phosphorus CaH4(PO4)2.2H2O 0.16 g Ca(NO3)2.4H2O Potassium KNO3 0.59 g NaNO3 Calcium CaSO4.2H2O 0.2 g K2SO4 CaH4(PO4)2.2H2O 0.71 g NaH2PO4.2H2O CaSO4.2H2O 0.16 g CaCl2 MgSO4.7H2O 0.21 g MgCl2.6H2O MgSO4.7H2O 0.17 g K2SO4 Sulfur Magnesium Soil culture Each student or pair of students will require: Seeds. Radish seeds may be preferred, but others could be tried. For example, rapid growing brassicas could be used: http://www.philipharris.co.uk/secondary/biology/plants-asorganisms/growing-mix/ Science & Plants for Schools: www.saps.org.uk Growing Plants in Different Media: p. 1 This document may be photocopied for educational use in any institution taking part in the SAPS programme. It may not be photocopied for any other purpose. Soil. A range of soils might be used and compared, including washed sand, vermiculite, vermiculite/peat mixture and any commercial seed compost low in nutrients. Nutrient solutions according to the nature of the investigation. Small container, e.g. film canister, with a hole cut in bottom and a diamond shaped piece of capillary matting for the wick (see SAPS The effects of different levels of minerals on plant growth). Water container with lid, e.g. plastic take-away food container, with a slot cut in the lid for the wick to pass through and dip into the nutrient solution. Water culture Each student or pair of students will require: Some seedlings, e.g. mung beans, barley or some other plant. A boiling tube and rack to stand it in. A piece of aluminium foil. Nutrient solutions according to the nature of the investigation. Floating culture Each student or pair of students will require: Ten healthy duckweed plants (Lemna) of similar size. Beaker, with plastic film to cover it Graph paper Nutrient solutions according to the nature of the investigation. Suppliers Parafilm can be purchased, for example, from Fisher Scientific (http://www.fisher.co.uk/product/index.php/12378039/Parafilm%20M/Sealing%20film/) Standard Sachs solutions available from Philip Harris. Teaching Notes Students may not be familiar with the types of compounds essential for healthy plant growth. Explain that they are compounds made from one or more atoms of certain elements. The three elements whose compounds are need in the largest quantities are: Nitrogen, N; Phosphorous, P; Potassium, K. The next three needed, but in small quantities are Sulfur, S; Magnesium, Mg; Calcium, Ca. Plants also need much smaller quantities of compounds of several other elements. In some places you may find the expression ‘essential elements’. This can be misleading unless students know that this is ‘shorthand’ for compounds made from one or more atoms of certain elements. It’s not the elements that are needed, it is certain ions made from atoms of these elements. For example, Nitrate, NO3-; Phosphate, PO43-; Potassium, K+; Sulfate, SO42-; Magnesium, Mg2+; Calcium, Ca2+ The nature of these ions is explored in the sheet Plant Nutrients. Science & Plants for Schools: www.saps.org.uk Growing Plants in Different Media: p. 2 This document may be photocopied for educational use in any institution taking part in the SAPS programme. It may not be photocopied for any other purpose.