Teaching Genetic Linkage and Recombination through Mapping with Molecular Markers
Lisa McDonnell and Jennifer Klenz
In-class HANDOUT A1: Predict the F2 data if there is genetic linkage
On your flip chart, draw a punnet square to
predict the F2 genotypes and phenotypes if
there is genetic linkage between the G
molecular marker and the locus involved in
drought tolerance. You should label each
offspring with the expected phenotype,
which includes if the plant is drought tolerant
or sensitive. Phenotype also includes the
banding pattern of the molecular marker.
Hint, you may want to assume a hypothetical
linkage distance, such as 20 map units, to
help you predict the frequency at which you’ll
see each banding pattern.
Which bands are parental? Recombinant?
Summarize your predictions: Which banding patterns will be observed if you are examining
the drought tolerant F2 only or the not-tolerant (wilting) F2 only. Approximately what
frequency will each banding pattern be observed in the populations?
A. Which bands are parental? Label them on the gel above.
B. Which bands are recombinant? Label them on the gel above.
Teaching Genetic Linkage and Recombination through Mapping with Molecular Markers
Lisa McDonnell and Jennifer Klenz
C. If there was genetic linkage, which plants would you use to determine the linkage
between the marker and the drought tolerance locus? Why?