1 2 The saprolite layer had been subterranean and previously unexposed, but had 3 been exposed several weeks before by a new road cutting. It had a coarse sand texture 4 with the original quartz mineral grains and micas/feldspar crystals in situ, closely 5 resembling the original rock, but crumbling in the hand due to past weathering. 6 Localized areas of haematite staining were present. No roots were present at the level 7 where we sampled (although some had penetrated the upper 50cm or so of saprolite), 8 and the saprolite overall had a white/pale yellow color. We sampled the saprolite at 9 points spaced 10m apart along the wall of the road cutting, 1 m vertical depth below 10 soil level and 30 cm horizontally into the exposed wall of the cutting, using a sterile 11 soil corer. In this photograph multiple coring sites are shown, of which only one was 12 used for a DNA extraction and PCR for archaea. 1/1