Chemical speciation of trace metals in sediment and their bioaccumulation in fish species of three urban rivers around Dhaka City, Bangladesh Md Saiful Islam, Md Kawser Ahmed, Mohammad Raknuzzaman, Md Habibullah-Al-Mamun and Shigeki Masunaga Table S1 Basic information of sampling sites Site ID Name of the site Possible pollution source T1 Tongi bazar Cotton mill, brick field, hatcheries, dumping sites for garments waste, pharmaceutical industries observed. T2 Ashulia Textiles, cotton mills, garbage dumping, pharmaceutical industries located to this site. T3 Gabtoli bridze This site is traffic junction, chemical incineration, burning and dumping of industrial effluents, brick fields located to this site. B1 Basila The main leather tanning zone, about 270 tanneries on an area of 4 km2, industrial waste dumping, dyeing industries observed B2 Kamrangirchar Urban waste dumping and burning, lead smelting factory, electroplating and city waste sewerage line also connected near this site. B3 Buriganga Bridge It is one of the major industrial location of Dhaka city with units of metals processing, global heavy chemicals Ltd., distillery, paper, textile, glass, electroplating, chemical, plastic, engineering, battery making etc. S1 Rupgang Agriculture field, oil factory, jute mills, cement factory and ship making factory also located to this sampling site. S2 Demra bridze Waste dispose channel, jute mill, cement factory, glass factory, oil mill observed at this site. S3 Shidhirgang It is downstream site of Kachpur industrial area and located near Shidhirgang power station. Waste burning, cement, textiles, spinning, paper and jute mills located to this site. Table S2 Measured and certified values of heavy metal concentration, as mg/kg dry weight, in standard reference material of NMIJ CRM 7303-a lake sediment and DORM-2 – dogfish muscle NMIJ CRM 7303-a lake sediment DORM-2 – dogfish muscle Certified value Measured value (n=3) Recovery (%) Certified value Measured value (n=3) Cr 39.1±2.8 39.2±0.2 100 34.7±5.5 33.9±2.2 Ni 21.8±2.5 21.6±0.4 99 19.4±3.1 19.1±1.1 Cu 23.1±3.1 23.3±0.6 101 2.34±0.16 2.52±0.10 As 8.6±1.0 8.5±0.1 99 18±1.1 17.7±1.6 Cd 0.342±0.017 0.341±0.010 100 0.043 ± 0.008 0.044±0.004 Pb 31.3±1.1 31.7±0.3 101 0.065 ± 0.007 0.068±0.005 Metal Reco very (%) 98 98 108 98 102 105