Name __________________________________ Test Date_________________ CELL STRUCTURE AND FUNCTION I. DISCOVERY OF CELLS A. History of Microscopes The invention and development of the microscope in the 1600’s enabled scientists to discover and study cells - basic unit of structure and function in in all living things 1. Anton von Leeuwenhoek – was the first to try stacking several lenses_ together to view tiny objects. He looked at pond water_ through his lenses and became known as the first scientist to describe living cells as seen through a microscope. 2. Robert Hooke - In 1665, he used a _microscope_ to examine thin slices of cork and then described what he saw and called them _cells__. He chose the name “cells” because the chambers he saw reminded him of the rooms in a monastery which were called cells. B. Cell Theory Nearly a century after Hooke’s findings, several other scientists discoveries led to the formation of the _cell theory_. 1. Matthias Schleiden (botanist) & Theodor Schwann (zoologist) – together they reached the conclusion that ____all____ living things were composed of cells_. 2. Rudolf Virchow – elaborated on Schleiden & Schwann’s proposal and added “omnia cellula e cellula” : all cells come from cells_ These discoveries, confirmed by other biologists, are summarized in the cell theory, a fundamental concept in Biology….. The cell theory states that: a. All organisms are ____composed of cells______ b. Cells are the smallest working units of life. c. All cells come from ____pre-existing cells________ II. TYPES OF CELLS Living organisms are made of either _prokaryotic__ or _eukaryotic_cells – the two major kinds of cells which can be distinguished by _structural organization__ PROKARYOTIC _Bacteria_____; Divided into two kingdoms, _Archaebacteria____ and _Eubacteria________ _No_ true nucleus; Lack nuclear envelope Genetic material present in_nucleoid___________ region EUKARYOTIC All organisms other than _bacteria______; Found in kingdoms _Protista____, _Fungi__, _Plantae___, and _Animalia______ _True____ nucleus surrounded membrane known as nuclear envelope Genetic material contained within_nucleus______________ __Lack_ membrane-bound organelles _Contain____ membrane-bound organelles A. Prokaryotic Cells – All Unicellular 1. Eubacteria – “True” Bacteria; Ex. include E.coli,Streptococcus. 2. Archaebacteria – “Ancient” bacteria; live in extreme environments (salty, hot, acidic); Ex. methanogens B. Eukaryotic Cells – Found in Unicellular and Multicellular organisms 1. Protista – ex. Amoeba, Euglena, Paramecium 2. Fungi - ex. Penicillium, yeasts, molds, mushrooms. 3. Plants – ex. Mosses, ferns, flowering plants. 4. Animals – ex. Sponges, worms, snails, insects, mammals. III. C. Microscopes - There are several types of modern microscopes: 1. Compound light microscope – More than one _lens (ocular & objective)________ uses light bent through lenses to magnify objects. 2. Electron Microscope – Uses _beams of electrons_________ . This enhances the resolution and magnification to allow researchers to study _cell structures____. There are two types of electron microscopes: a. Transmission Electron Microscope or _TEM______________- electrons are Transmitted _through_____the specimen; used to study _internal___ cell structures. b. Scanning Electron Microscope or _SEM_____ - uses electrons to trace the _surface_____ of the specimen; provides _depth___ of field or 3-D view. III. CELL BOUNDARIES A. Cell Wall Cell Walls are the outer most boundary in _bacteria_, _plants_, and _fungi_. They are not found in __animal cells_____. The primary function of the cell wall is to _provide support and structure__. Plants have cell walls made of _cellulose___ Fungi have cell walls made of _chitin____. Eubacteria have cell walls made of _peptidoglycan_____. The cell walls of _Archaebacteria___ are composed of other _polysaccharides_____. B. Cell Membrane Every cell is surrounded by the cell membrane. Its function is to maintain homeostasis__ in the cell by separating and protecting the cell from its environment. It also regulates exchange with the environment. The cell membrane is also called the __plasma membrane_. It is _selectively permeable_ which means that it allows some substances to pass through; acts a barrier to others. IV. INSIDE A EUKARYOTIC CELL Within the cell membrane, the cell is composed of the nucleus with its corresponding structures, the _nucleolus_ and _nuclear envelope__. The cytoplasm includes all the rest of the material inside the cell membrane. The cytoplasm includes two components: Cytosol – a semi-gelatinous substance that contains dissolved nutrients and wastes Organelles – means “little organs”. Each has a specific role in the overall function of the cell. Illustration Structure Type of Cell Characteristics & Function _Control center____ of the cell. Contains Nucleus Euk most of cell’s DNA; stored as _chromatin___ (_DNA_______ wrapped in _protein_____) Small, dense region in the nucleus. Nucleolus Euk Involved in the synthesis of _ribosomes_____ which are required for _protein______ synthesis. Nuclear Double membrane, each consisting of a Envelope Euk _phospholipid bilayer__. Perforated by nuclear _pores______ which allow _RNA______ to leave the nucleus Tiny, non-membrane bound organelles Ribosomes Pro + Euk located on _endoplasmic reticulum_____ (bound) or suspended in _cytosol_______ (free). Free ribosomes help manufacture _proteins_____ that will stay in the cell; bound ribosomes help make proteins that will transported out of cell Extensive network continuous with _nuclear envelope______. Called “rough” Rough Euk due to presence of _ribosomes____ all Endoplasmic along the membrane. Function of the rough Reticulum ER is to _modify____ and transport _proteins_____. Most of these proteins are packaged into _vesicles_____ (bubbles or sacs made from membrane) and shuttled to the _Golgi apparatus________ Euk Similar to rough ER in structure, except Smooth that it lacks _ribosomes______. Smooth Endoplasmic ER functions in the synthesis of Reticulum _lipids_____, metabolizes _glycogen___, detoxifies _poisons_____ and _toxins___. Also stores _calcium__needed for muscle contractions. Golgi apparatus Euk Lysosome Euk – Animal Cells Vacuole Euk Mitochondria Chloroplast Centrioles Euk Flattened, round sacs that look like a sack of _pancakes______. Receives, modifies, and ships products by way of _vesicles_______ into the _cytosol_________ Found in _animal ___ cells only?? Membrane-bound sacs containing _hydrolytic enzymes___ that break down _biomolecules_______ and _recycle____ used cell components. Also used as defense against _bacteria________ and _viruses______ Sacs that may be used as _storage_____ for water, ions, wastes. Plants have a large central vacuole. Double-membrane organelle with inner folds called _cristae_____. Uses _glucose_______ to manufacture energy in the form of _ATP_____. Mitochondria have their own _DNA____. Euk – Found in _plant____ cells. Contain green Plant Cells pigment called _chlorophyll_________ and their own _DNA__________. Chloroplasts harvest energy from the _sun____ to produce _glucose____ through _photosynthesis______. Euk – Animal Cells Found only in _animal__ cells. Bundles of _microtubules________ that play a role in _cell division_____. Cytoskeleton Euk VI. THE PROKARYOTIC CELL Cell wall - 6 Cell membrane - 4 DNA - 3 Cytosol - 8 Ribosomes - 5 Capsule - 7 Pili – 2 Flagella - 1 8 Network of _protein____ fibers known as _microtubules______ and _microfilaments_________. Anchor _organelles______ and provide _support______. Also provide motility for some cells in the form of _cilia______ or _flagella________. More extensive cytoskeleton found in _animal____ cells. VII. THE EUKARYOTIC CELL KEY TO EUKARYOTIC CELL LABELING A. nucleus B. nuclear envelope C. chromatin D. nucleolus E. mitochondria F. Golgi apparatus G. rough endoplasmic reticulum H. ribosome I. vacuole J. cytosol K. cytoskeleton L. cell membrane M. smooth endoplasmic reticulum N. centrioles O. lysosome P. cell wall Q. chloroplast