Lab ____: The Reproductive Structures in a Flower Lab skills: 1, 3, 6, 8, 13, 18, 19, 26, 28 Background: Flowers are the reproductive organs of angiosperms, or flowering plants. Flowers vary in size, color and arrangement. All flowers, regardless of variety, have the function of seed formation and are responsible for the production of more plants. Flowers contain both non-reproductive and reproductive structures. In this investigation you will examine a flower and identify its non-reproductive parts and its reproductive parts. You will also observe the male and female sex cells produced by a flower underneath a microscope. Hypothesis: If flowers are reproductive structures then there must be male parts and / or female parts within them. Procedure: Part A – Observing the External Anatomy of a Flower 1. Refer to Figure 1 as you examine the flower. Identify the receptacle, or structure to which all other flower parts are attached. Note the sepals, which are small, leaf-like structures above the receptacle. All of the sepals together are called the calyx. Observe the brightly colored petals inside the calyx. All of the petals together are called the corolla. These structures make up the non-reproductive structures of a flower. 2. Record the number and color of both the sepals and the petals in the first table in your results section. Part B – Reproductive Structures of a Flower 3. Using Figure 2 identify the male reproductive structures, or stamens. Each stamen consists of an anther and a filament. 4. Identify the female reproductive structure, or pistil. Each pistil consists of an ovary, a stigma, and a style. 5. Sketch the flower you observed on your answer sheet. Label the stamen, anther, filament, pistil, ovary, stigma and style. 6. With scissors or forceps carefully remove the petals from the flower. Make sure you do not damage any reproductive structures. 7. With forceps, remove the stamens and place them on a sheet of unlined white paper. Flower Reproductive Structures Lab Page 1 8. Use a hand lens to get a close look at the male reproductive structures of the flower. Sketch what you see and label the pollen, anther, and filament on your stamen drawing on your answer sheet. Set the stamens aside for use in Part C. 9. Carefully remove the pistil using a razor. Cut the ovary in half as its widest part as shown in Figure 3. 10. Observe the inside of the ovary. Note the number and arrangement of the ovary chambers. The white, oval objects inside the chambers are the ovules (or eggs). 11. Sketch a cross section of the ovary showing the number and arrangement of the chambers on your answer sheet. Label the ovules, ovary walls and chambers. Set the pistil aside for Part C. Part C – The Gametes of a Flower 12. With forceps, remove an ovule from the dissected ovary. Make a wet-mount slide of the ovule. 13. Observe the slide at low power and calculate the magnification. Sketch what the ovule looks like in your results section and record the magnification at which you viewed it. 14. Gently brush an anther from one of the stamens across the stigma of the pistil. With a hand lens look for pollen grains that may have stuck to the stigma. 15. Make a wet-mount slide of pollen and observe it at high power. Sketch what the pollen looks like in your results section and record the magnification at which you viewed it. Analysis: A. List the function of each of the following parts and note whether the parts comprise the pistil or the stamen. 1 – anther 2 – filament 3 – ovary 4 – ovule 5 – pollen 6 – stigma 7 – style B. Why does a flower have more pollen grains than ovules? C. Explain why heavy rainfall on a fruit orchard in the spring might result in a poor fruit yield in the fall. D. For what biological reason might farmers object to people picking apple blossoms during the apple blossom season? E. Flowers that are pollinated by the wind have smaller petals and sepals than flowers that are pollinated by insects or animals. Why are small petals and sepals an advantage to these flowers? F. Why do flowers that are pollinated by insects have large, brightly colored petals? Flower Reproductive Structures Lab Page 2 Name(s) __________________________________________________________________________ Period ________ Reproductive Structures in a Flower Lab Results Flower common name Flower classification name Labeled sketch of a flower Number of sepals Color of sepals Number of petals Color of petals Labeled sketch of the male reproductive parts of a flower Labeled sketch of the female reproductive parts of a flower Ovules @ low power Pollen @ high power Magnification: ___________________ Magnification: ___________________ Flower Reproductive Structures Lab Page 3 Part A… Observe sepals and petals here! Part B… Observe the flower here! Flower Reproductive Structures Lab Page 4 Part B… Observe the male reproductive flower parts here! Part B… Observe the female flower parts here! Flower Reproductive Structures Lab Page 5 Part C… Observe the ovules here! Part C… Observe the pollen grains here! Flower Reproductive Structures Lab Page 6